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. 1974 Dec 1;63(3):759–772. doi: 10.1083/jcb.63.3.759

SYNTHESIS OF CHLOROPLAST MEMBRANE POLYPEPTIDES DURING SYNCHRONOUS GROWTH OF CHLAMYDOMONAS REINHARDTII

David P Beck 1, R P Levine 1
PMCID: PMC2109360  PMID: 4436383

Abstract

The synthesis of the major chloroplast membrane polypeptides has been studied during synchronous growth of Chlamydomonas reinhardtii. Under these conditions, chlorophyll is synthesized during the latter part of the light period and cell division takes place during the dark period. The profile of the chloroplast membrane polypeptides of C. reinhardtii has been well characterized and shown to contain two major classes by size (Hoober, J. 1970. J. Biol. Chem. 245:4327). Polypeptides of group I have a mol wt range of 50,000–55,000 daltons. The second region consists of at least three polypeptide groups, IIa, IIb, and IIc, having mol wt of 40,000, 31,000, and 27,000 daltons, respectively. The synthesis of these polypeptides has been measured using a double-labeling technique and a computer-aided statistical analysis. The rate of labeling of group I polypeptides is highest during the early light period and decreases after 6 h of growth. Group IIa is labeled from the beginning of the light period, but little synthesis of IIb occurs before 3 h, and significant amounts of label are not found in IIc before 5 h of growth. After approximately 8 h of light, groups IIb and IIc are synthesized at rates significantly greater than those of the other membrane polypeptides. The synthesis of the major polypeptide groups ceases in the dark. We conclude that the biosynthesis of the chloroplast membranes is a sequential or stepwise process.

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Selected References

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  1. Alberte R. S., Thornber J. P., Naylor A. W. Biosynthesis of the photosystem I chlorophyll-protein complex in greening leaves of higher plants. Proc Natl Acad Sci U S A. 1973 Jan;70(1):134–137. doi: 10.1073/pnas.70.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. M., Levine R. P. The relationship between chlorophyll-protein complexes and chloroplast membrane polypeptides. Biochim Biophys Acta. 1974 Jul 25;357(1):118–126. doi: 10.1016/0005-2728(74)90117-0. [DOI] [PubMed] [Google Scholar]
  3. Arias I. M., Doyle D., Schimke R. T. Studies on the synthesis and degradation of proteins of the endoplasmic reticulum of rat liver. J Biol Chem. 1969 Jun 25;244(12):3303–3315. [PubMed] [Google Scholar]
  4. Armstrong J. J., Moll B., Surzycki S. J., Levine R. P. Genetic transcription and translation specifying chloroplast components in Chlamydomonas reinhardi. Biochemistry. 1971 Feb 16;10(4):692–701. doi: 10.1021/bi00780a022. [DOI] [PubMed] [Google Scholar]
  5. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. De Petrocellis B., Siekevitz P., Palade G. E. Changes in chemical composition of thylakoid membranes during greening of the y-1 mutant of Chlamydomonas reinhardi. J Cell Biol. 1970 Mar;44(3):618–634. doi: 10.1083/jcb.44.3.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dehlinger P. J., Schimke R. T. Size distribution of membrane proteins of rat liver and their relative rates of degradation. J Biol Chem. 1971 Apr 25;246(8):2574–2583. [PubMed] [Google Scholar]
  8. Dietrich W. E., Jr, Thornber J. P. The P700-chlorophyll -protein of a blue-green alga. Biochim Biophys Acta. 1971 Sep 7;245(2):482–493. doi: 10.1016/0005-2728(71)90164-2. [DOI] [PubMed] [Google Scholar]
  9. Goldberg I., Ohad I. Biogenesis of chloroplast membranes. IV. Lipid and pigment changes during synthesis of chloroplast membranes in a mutant of Chlamydomonas reinhardi y-1. J Cell Biol. 1970 Mar;44(3):563–571. doi: 10.1083/jcb.44.3.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goldberg I., Ohad I. Biogenesis of chloroplast membranes. V. A radioautographic study of membrane growth in a mutant of Chlamydomonas reinhardi y-1. J Cell Biol. 1970 Mar;44(3):572–591. doi: 10.1083/jcb.44.3.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoober J. K. A major polypeptide of chloroplast membranes of Chlamydomonas reinhardi. Evidence for synthesis in the cytoplasm as a soluble component. J Cell Biol. 1972 Jan;52(1):84–96. doi: 10.1083/jcb.52.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hoober J. K., Siekevitz P., Palade G. E. Formation of chloroplast membranes in Chlamydomonas reinhardi y-1. Effects of inhibitors of protein synthesis. J Biol Chem. 1969 May 25;244(10):2621–2631. [PubMed] [Google Scholar]
  14. Hoober J. K. Sites of synthesis of chloroplast membrane polypeptides in Chlamydomonas reinhardi y-1. J Biol Chem. 1970 Sep 10;245(17):4327–4334. [PubMed] [Google Scholar]
  15. Howell S. H., Moudrianakis E. N. Function of the "quantasome" in photosynthesis: structure and properties of membrane-bound particle active in the dark reactions of photophosphorylation. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1261–1268. doi: 10.1073/pnas.58.3.1261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Levine R. P., Burton W. G., Duram H. A. Membrane polypeptides associated with photochemical systems. Nat New Biol. 1972 Jun 7;237(75):176–177. doi: 10.1038/newbio237176a0. [DOI] [PubMed] [Google Scholar]
  18. Levine R. P., Duram H. A. The polypeptides of stacked and unstacked Chlamydomonas reinhardi chloroplast membranes and their relation to photsystem II activity. Biochim Biophys Acta. 1973 Dec 14;325(3):565–572. doi: 10.1016/0005-2728(73)90216-8. [DOI] [PubMed] [Google Scholar]
  19. Levine R. P., Togasaki R. K. A mutant strain of Chlamydomonas reinhardi lacking ribulose diphosphate carboxylase activity. Proc Natl Acad Sci U S A. 1965 May;53(5):987–990. doi: 10.1073/pnas.53.5.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mindich L. Control of fatty acid synthesis in bacteria. J Bacteriol. 1972 Apr;110(1):96–102. doi: 10.1128/jb.110.1.96-102.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mindich L. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol. 1970 Apr 28;49(2):415–432. doi: 10.1016/0022-2836(70)90254-8. [DOI] [PubMed] [Google Scholar]
  22. Mindich L. Membrane synthesis in Bacillus subtilis. II. Integration of membrane proteins in the absence of lipid synthesis. J Mol Biol. 1970 Apr 28;49(2):433–439. doi: 10.1016/0022-2836(70)90255-x. [DOI] [PubMed] [Google Scholar]
  23. Ogawa T., Obata F., Shibata K. Two pigment proteins in spinach chloroplasts. Biochim Biophys Acta. 1966 Feb 7;112(2):223–234. doi: 10.1016/0926-6585(66)90323-2. [DOI] [PubMed] [Google Scholar]
  24. Ohad I., Siekevitz P., Palade G. E. Biogenesis of chloroplast membranes. I. Plastid dedifferentiation in a dark-grown algal mutant (Chlamydomonas reinhardi). J Cell Biol. 1967 Dec;35(3):521–552. doi: 10.1083/jcb.35.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ohad I., Siekevitz P., Palade G. E. Biogenesis of chloroplast membranes. II. Plastid differentiation during greening of a dark-grown algal mutant (Chlamydomonas reinhardi). J Cell Biol. 1967 Dec;35(3):553–584. doi: 10.1083/jcb.35.3.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Omura T., Siekevitz P., Palade G. E. Turnover of constituents of the endoplasmic reticulum membranes of rat hepatocytes. J Biol Chem. 1967 May 25;242(10):2389–2396. [PubMed] [Google Scholar]
  27. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  28. SAGER R., PALADE G. E. Chloroplast structure in green and yellow strains of Chlamydomonas. Exp Cell Res. 1954 Nov;7(2):584–588. doi: 10.1016/s0014-4827(54)80107-8. [DOI] [PubMed] [Google Scholar]
  29. Schor S., Siekevitz P., Palade G. E. Cyclic Changes in Thylakoid Membranes of Synchronized Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1970 May;66(1):174–180. doi: 10.1073/pnas.66.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sueoka N. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):83–91. doi: 10.1073/pnas.46.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. TROWN P. W. AN IMPROVED METHOD FOR THE ISOLATION OF CARBOXYDISMUTASE. PROBABLE IDENTITY WITH FRACTION I PROTEIN AND THE PROTEIN MOIETY OF PROTOCHLOROPHYLL HOLOCHROME. Biochemistry. 1965 May;4:908–918. doi: 10.1021/bi00881a018. [DOI] [PubMed] [Google Scholar]
  32. Thornber J. P., Gregory R. P., Smith C. A., Bailey J. L. Studies on the nature of the chloroplast lamella. I. Preparation and some properties of two chlorophyll-protein complexes. Biochemistry. 1967 Feb;6(2):391–396. doi: 10.1021/bi00854a004. [DOI] [PubMed] [Google Scholar]
  33. Thornber J. P., Stewart J. C., Hatton M. W., Bailey J. L. Studies on the nature of chloroplast lamellae. II. Chemical composition and further physical properties of two chlorophyll-protein complexes. Biochemistry. 1967 Jul;6(7):2006–2014. doi: 10.1021/bi00859a019. [DOI] [PubMed] [Google Scholar]
  34. Wilson G., Fox C. F. Biogenesis of microbial transport systems: evidnce for coupled incorporation of newly synthesized lipids and proteins into membrane. J Mol Biol. 1971 Jan 14;55(1):49–60. doi: 10.1016/0022-2836(71)90280-4. [DOI] [PubMed] [Google Scholar]

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