Skip to main content
PMC home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1974 Aug 1;62(2):566–574. doi: 10.1083/jcb.62.2.566

ALTERATIONS IN MORPHOLOGY OF DEVELOPING MICROVILLI ELICITED BY CYTOCHALASIN B

Studies of Embryonic Chick Intestine in Organ Culture

David R Burgess 1, Robert D Grey 1
PMCID: PMC2109406  PMID: 4426920

Full Text

The Full Text of this article is available as a PDF (672.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonneville M. A., Weinstock M. Brush border development in the intestinal absorptive cells of Xenopus during metamorphosis. J Cell Biol. 1970 Jan;44(1):151–171. doi: 10.1083/jcb.44.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brunser O., Luft H. J. Fine structure of the apex of absorptive cell from rat small intestine. J Ultrastruct Res. 1970 May;31(3):291–311. doi: 10.1016/s0022-5320(70)90133-4. [DOI] [PubMed] [Google Scholar]
  3. Carter S. B. Effects of cytochalasins on mammalian cells. Nature. 1967 Jan 21;213(5073):261–264. doi: 10.1038/213261a0. [DOI] [PubMed] [Google Scholar]
  4. Cloney R. A. Cytoplasmic filaments and morphogenesis: effects of cytochalasin B on contractile epidermal cells. Z Zellforsch Mikrosk Anat. 1972;132(2):167–192. doi: 10.1007/BF00307009. [DOI] [PubMed] [Google Scholar]
  5. Cloney R. A. Cytoplasmic filaments and morphogenesis: the role of the notochord in ascidian metamorphosis. Z Zellforsch Mikrosk Anat. 1969;100(1):31–53. doi: 10.1007/BF00343819. [DOI] [PubMed] [Google Scholar]
  6. Cohn R. H., Banerjee S. D., Shelton E. R., Bernfield M. R. Cytochalasin B: lack of effect on mucopolysaccharide synthesis and selective alterations in precursor uptake. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2865–2869. doi: 10.1073/pnas.69.10.2865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ebstensen R. D., Plagemann P. G. Cytochalasin B: inhibition of glucose and glucosamine transport. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1430–1434. doi: 10.1073/pnas.69.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fine R. E., Bray D. Actin in growing nerve cells. Nat New Biol. 1971 Nov 24;234(47):115–118. doi: 10.1038/newbio234115a0. [DOI] [PubMed] [Google Scholar]
  9. Grey R. D. Morphogenesis of intestinal villi. I. Scanning electron microscopy of the duodenal epithelium of the developing chick embryo. J Morphol. 1972 Jun;137(2):193–213. doi: 10.1002/jmor.1051370206. [DOI] [PubMed] [Google Scholar]
  10. Ishikawa H., Bischoff R., Holtzer H. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol. 1969 Nov;43(2):312–328. [PMC free article] [PubMed] [Google Scholar]
  11. Ito S. The enteric surface coat on cat intestinal microvilli. J Cell Biol. 1965 Dec;27(3):475–491. doi: 10.1083/jcb.27.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jessen H., Behnke O., Wingstrand K. G., Rostgaard J. Actin-like filaments in the acrosomal apparatus of spermatozoa of a sea urchin. Exp Cell Res. 1973 Jul;80(1):47–54. doi: 10.1016/0014-4827(73)90273-5. [DOI] [PubMed] [Google Scholar]
  13. Kato Y. A modified method for chorioallantoic membrane grafting. Transplantation. 1970 Oct;10(4):354–358. [PubMed] [Google Scholar]
  14. Kletzien R. F., Perdue J. F. The inhibition of sugar transport in chick embryo fibroblasts by cytochalasin B. Evidence for a membrane-specific effect. J Biol Chem. 1973 Jan 25;248(2):711–719. [PubMed] [Google Scholar]
  15. Lecount T. S., Grey R. D. Transient shortening of microvilli induced by cycloheximide in the duodenal epithelium of the chicken. J Cell Biol. 1972 May;53(2):601–605. doi: 10.1083/jcb.53.2.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lieberman M., Manasek F. J., Sawanobori T., Johnson E. A. Cytochalasin B: its morphological and electrophysiological actions on synthetic strands of cardiac muscle. Dev Biol. 1973 Apr;31(2):380–403. doi: 10.1016/0012-1606(73)90273-x. [DOI] [PubMed] [Google Scholar]
  17. Mizel S. B., Wilson L. Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B. J Biol Chem. 1972 Jun 25;247(12):4102–4105. [PubMed] [Google Scholar]
  18. Mukherjee T. M., Staehelin L. A. The fine-structural organization of the brush border of intestinal epithelial cells. J Cell Sci. 1971 May;8(3):573–599. doi: 10.1242/jcs.8.3.573. [DOI] [PubMed] [Google Scholar]
  19. OVERTON J., SHOUP J. FINE STRUCTURE OF CELL SURFACE SPECIALIZATIONS IN THE MATURING DUODENAL MUCOSA OF THE CHICK. J Cell Biol. 1964 Apr;21:75–85. doi: 10.1083/jcb.21.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Overton J. Fine structure of the free cell surface in developing mouse intestinal mucosa. J Exp Zool. 1965 Jul;159(2):195–201. doi: 10.1002/jez.1401590205. [DOI] [PubMed] [Google Scholar]
  21. Plagemann P. G., Estensen R. D. Cytochalasin B. VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells. J Cell Biol. 1972 Oct;55(1):179–185. doi: 10.1083/jcb.55.1.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schroeder T. E. Actin in dividing cells: contractile ring filaments bind heavy meromyosin. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1688–1692. doi: 10.1073/pnas.70.6.1688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schroeder T. E. The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B. Z Zellforsch Mikrosk Anat. 1970;109(4):431–449. [PubMed] [Google Scholar]
  24. Spooner B. S., Ash J. F., Wrenn J. T., Frater R. B., Wessells N. K. Heavy meromyosin binding to microfilaments involved in cell and morphogenetic movements. Tissue Cell. 1973;5(1):37–46. doi: 10.1016/s0040-8166(73)80004-7. [DOI] [PubMed] [Google Scholar]
  25. Spooner B. S., Wessells N. K. An analysis of salivary gland morphogenesis: role of cytoplasmic microfilaments and microtubules. Dev Biol. 1972 Jan;27(1):38–54. doi: 10.1016/0012-1606(72)90111-x. [DOI] [PubMed] [Google Scholar]
  26. Spooner B. S., Wessells N. K. Effects of cytochalasin B upon microfilaments involved in morphogenesis of salivary epithelium. Proc Natl Acad Sci U S A. 1970 Jun;66(2):360–361. doi: 10.1073/pnas.66.2.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Starre-van der Molen L. G., de Priester W. Brush-border formation in the midgut of an insect, Calliphora erythrocephala Meigen. The formation of microvilli in the midgut during embryonic development. Z Zellforsch Mikrosk Anat. 1972;125(3):295–305. doi: 10.1007/BF00306628. [DOI] [PubMed] [Google Scholar]
  28. Tilney L. G., Hatano S., Ishikawa H., Mooseker M. S. The polymerization of actin: its role in the generation of the acrosomal process of certain echinoderm sperm. J Cell Biol. 1973 Oct;59(1):109–126. doi: 10.1083/jcb.59.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tilney L. G., Mooseker M. Actin in the brush-border of epithelial cells of the chicken intestine. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2611–2615. doi: 10.1073/pnas.68.10.2611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. doi: 10.1126/science.171.3967.135. [DOI] [PubMed] [Google Scholar]
  31. Wrenn J. T. An analysis of tubular gland morphogenesis in chick oviduct. Dev Biol. 1971 Nov;26(3):400–415. doi: 10.1016/0012-1606(71)90072-8. [DOI] [PubMed] [Google Scholar]
  32. Wrenn J. T., Wessells N. K. Cytochalasin B: effects upon microfilaments involved in morphogenesis of estrogen-induced glands of oviduct. Proc Natl Acad Sci U S A. 1970 Jul;66(3):904–908. doi: 10.1073/pnas.66.3.904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yamada K. M., Spooner B. S., Wessells N. K. Ultrastructure and function of growth cones and axons of cultured nerve cells. J Cell Biol. 1971 Jun;49(3):614–635. doi: 10.1083/jcb.49.3.614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yang Y. Z., Perdue J. F. Contractile proteins of cultured cells. I. The isolation and characterization of an actin-like protein from cultured chick embryo fibroblasts. J Biol Chem. 1972 Jul 25;247(14):4503–4509. [PubMed] [Google Scholar]
  35. de Laat S. W., Luchtel D., Bluemink J. G. The action of cytochalasin B during egg cleavage in Xenopus laevis: dependence on cell membrane permeability. Dev Biol. 1973 Mar;31(1):163–177. doi: 10.1016/0012-1606(73)90327-8. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES