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. 1975 Sep 1;66(3):676–680. doi: 10.1083/jcb.66.3.676

Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique

PMCID: PMC2109466  PMID: 51024

Abstract

The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.

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Selected References

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