Abstract
The developing chick retina from stages 39-45 has been examined by biochemical and electron microscope techniques. The levels of rhodopsin contained in the maturing chick retina were evaluated by detergent extraction and correlated with rod outer segment formation. It was found that the appearance of rhodopsin in significant levels preceded outer segment formation by at least 2 days, thus implying that rhodopsin is synthesized in the receptor cell inner segment and translocated to the outer limb when disk membrane biogenesis occurs. The level of rhodopsin continues to rise as the rod outer segment develops. Development of both rods and cones originates and proceeds most rapidly in the fundus or central region and proceeds toward the periphery. In general, rod outer segments were noted to develop far more rapidly than cone outer segments.
Full Text
The Full Text of this article is available as a PDF (2.4 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bargoot F. G., Williams T. P., Beidler L. M. The localization of radioactive amino acid taken up into the outer segments of frog (Rana pipiens) rods. Vision Res. 1969 Mar;9(3):385–391. doi: 10.1016/0042-6989(69)90085-6. [DOI] [PubMed] [Google Scholar]
- Hall M. O., Bok D., Bacharach A. D. Biosynthesis and assembly of the rod outer segment membrane system. Formation and fate of visual pigment in the frog retina. J Mol Biol. 1969 Oct 28;45(2):397–406. doi: 10.1016/0022-2836(69)90114-4. [DOI] [PubMed] [Google Scholar]
- Hall M. O., Bok D., Bacharach A. D. Visual pigment renewal in the mature frog retina. Science. 1968 Aug 23;161(3843):787–789. doi: 10.1126/science.161.3843.787. [DOI] [PubMed] [Google Scholar]
- Matsubara T., Miyata M., Mizuno K. Radioisotopic studies on renewal of opsin. Vision Res. 1968 Sep;8(9):1139–1143. doi: 10.1016/0042-6989(68)90023-0. [DOI] [PubMed] [Google Scholar]
- Meyer D. B., Cooper T. G. The visual cells of the chicken as revealed by phase contrast microscopy. Am J Anat. 1966 May;118(3):723–734. doi: 10.1002/aja.1001180303. [DOI] [PubMed] [Google Scholar]
- Nilsson S. E., Crescitelli F. A correlation of ultrastructure and function in the developing retina oof the frog tadpole. J Ultrastruct Res. 1970 Jan;30(1):87–102. doi: 10.1016/s0022-5320(70)90066-3. [DOI] [PubMed] [Google Scholar]
- SHEN S. C., GREENFIELD P., BOELL E. J. Localization of acetylcholinesterase in chick retina during histogenesis. J Comp Neurol. 1956 Dec;106(2):433–461. doi: 10.1002/cne.901060211. [DOI] [PubMed] [Google Scholar]
- WITKOVSKY P. AN ONTOGENETIC STUDY OF RETINAL FUNCTION IN THE CHICK. Vision Res. 1963 Nov;61:341–355. doi: 10.1016/0042-6989(63)90008-7. [DOI] [PubMed] [Google Scholar]
- Young R. W., Bok D. Participation of the retinal pigment epithelium in the rod outer segment renewal process. J Cell Biol. 1969 Aug;42(2):392–403. doi: 10.1083/jcb.42.2.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young R. W., Droz B. The renewal of protein in retinal rods and cones. J Cell Biol. 1968 Oct;39(1):169–184. doi: 10.1083/jcb.39.1.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young R. W. Passage of newly formed protein through the connecting cilium of retina rods in the frog. J Ultrastruct Res. 1968 Jun;23(5):462–473. doi: 10.1016/s0022-5320(68)80111-x. [DOI] [PubMed] [Google Scholar]