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. 1975 Feb 1;64(2):331–339. doi: 10.1083/jcb.64.2.331

Two improved methods for preparing ferritin-protein conjugates for electron microscopy

PMCID: PMC2109494  PMID: 803973

Abstract

Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin- antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.

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Selected References

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