Abstract
Nuclear contents or cytoplasm from Xenopus oocytes labeled with (35- S)methionine or (3-H)proline (donor oocytes) were reinjected into unlabeled oocytes (recipient oocytes). The radioactivity injected as nuclear contents was found to enter and accumulate in the recipient oocyte nucleus. In contrast, the radioactivity injected as cytoplasm was found to enter but not to accumulate in the recipient oocyte nucleus. Sodium dodecyl sulfate (SDS) gel electrophoresis of the nucleus and cytoplasm of donor oocytes revealed the existence of three classes of labeled proteins in these oocytes: those proteins found predominantly in the nucleus (N proteins), those found predominantly in the cytoplasm (C proteins), and those found in both the nucleus and cytoplasm at similar concentrations (B proteins). SDS gel electrophoresis of the nucleus and cytoplasm of recipient oocytes showed that N proteins entered and accumulated in the nucleus but that B proteins partitioned about equally between the nucleus and cytoplasm. A similar analysis of oocytes injected with labeled cytoplasm showed that C proteins did not enter the nucleus but again B proteins partitioned about equally between the nucleus and cytoplasm.
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Selected References
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- Arms K. Cytonucleoproteins in cleaving eggs of Xenopus laevis. J Embryol Exp Morphol. 1968 Nov;20(3):367–374. [PubMed] [Google Scholar]
- Bonner W. M. Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins. J Cell Biol. 1975 Feb;64(2):421–430. doi: 10.1083/jcb.64.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borun T. W., Scharff M. D., Robbins E. Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977–1983. doi: 10.1073/pnas.58.5.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dumont J. N. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J Morphol. 1972 Feb;136(2):153–179. doi: 10.1002/jmor.1051360203. [DOI] [PubMed] [Google Scholar]
- Gallwitz D., Mueller G. C. Histone synthesis in vitro by cytoplasmic microsomes from HeLa cells. Science. 1969 Mar 21;163(3873):1351–1353. doi: 10.1126/science.163.3873.1351. [DOI] [PubMed] [Google Scholar]
- Gurdon J. B. Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes. J Embryol Exp Morphol. 1968 Nov;20(3):401–414. [PubMed] [Google Scholar]
- Gurdon J. B. Nuclear transplantation and the control of gene activity in animal development. Proc R Soc Lond B Biol Sci. 1970 Dec 1;176(1044):303–314. doi: 10.1098/rspb.1970.0050. [DOI] [PubMed] [Google Scholar]
- Jelinek W., Goldstein L. Isolation and characterization of some of the proteins that shuttle between cytoplasm and nucleus in Amoeba proteus. J Cell Physiol. 1973 Apr;81(2):181–197. doi: 10.1002/jcp.1040810207. [DOI] [PubMed] [Google Scholar]
- KROEGER H., JACOB J., SIRLIN J. L. THE MOVEMENT OF NUCLEAR PROTEIN FROM THE CYTOPLASM TO THE NUCLEUS OF SALIVARY CELLS. Exp Cell Res. 1963 Aug;31:416–423. doi: 10.1016/0014-4827(63)90018-1. [DOI] [PubMed] [Google Scholar]
- Legname C., Goldstein L. Proteins in nucleocytoplasmic interactions. VI. Is there an artefact responsible for the observed shuttling of proteins between cytoplasm and nucleus in Amoeba proteus? Exp Cell Res. 1972 Nov;75(1):111–121. doi: 10.1016/0014-4827(72)90526-5. [DOI] [PubMed] [Google Scholar]
- Merriam R. W. Movement of cytoplasmic proteins into nuclei induced to enlarge and initiate DNA or RNA synthesis. J Cell Sci. 1969 Sep;5(2):333–349. doi: 10.1242/jcs.5.2.333. [DOI] [PubMed] [Google Scholar]
- Nemer M., Lindsay D. T. Evidence that the s-polysomes of early sea urchin embryos may be responsible for the synthesis of chromosomal histones. Biochem Biophys Res Commun. 1969 Apr 10;35(1):156–160. doi: 10.1016/0006-291x(69)90498-7. [DOI] [PubMed] [Google Scholar]