Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1975 Mar 1;64(3):668–681. doi: 10.1083/jcb.64.3.668

Endogenous peroxidase: specific marker enzyme for tissues displaying growth dependency on estrogen

PMCID: PMC2109545  PMID: 1171106

Abstract

Data derived from a correlated morphological and biochemical study suggest the following: (a) estradiol-17beta, diethylstilbestrol, the estrogen antagonists nafoxidine (Upjohn 11,000), and Parke Davis C1628 induce synthesis of an endogenous peroxidase in the epithelium of target tissues like the vagina, the cervix, the uterus, and in the acinar cells of the estrogen-dependent rat mammary tumor; (b) peroxidase is a "specific" secretory protein of the estrogen-sensitized uterine endometrium; (c) peroxidase synthesis is not a nonspecific response to steroid hormone action, since progesterone and testosterone do not induce its synthesis; (d) endogenous peroxidase is a possible diagnositc protein for the detection of estrogen-dependent growing tissues, including breast cancer; (e) movement of exogenous horseradish peroxidase from the interstitium to the uterine lumina is restricted by tight junctions located at the apices of epithelial cells. Estrogen and antagonists do not appear to influence the transepithelial movement of exogenous peroxidase into the lumen.

Full Text

The Full Text of this article is available as a PDF (5.7 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnea A., Gorski J. Estrogen-induced protein. Time course of synthesis. Biochemistry. 1970 Apr 28;9(9):1899–1904. doi: 10.1021/bi00811a006. [DOI] [PubMed] [Google Scholar]
  2. Beier H. M. Uteroglobin: a hormone-sensitive endometrial protein involved in blastocyst development. Biochim Biophys Acta. 1968 Jun 26;160(2):289–291. doi: 10.1016/0005-2795(68)90108-6. [DOI] [PubMed] [Google Scholar]
  3. Belding M. E., Klebanoff S. J., Ray C. G. Peroxidase-mediated virucidal systems. Science. 1970 Jan 9;167(3915):195–196. doi: 10.1126/science.167.3915.195. [DOI] [PubMed] [Google Scholar]
  4. Callantine M. R., Humphrey R. R., Lee S. L., Windsor B. L., Schottin N. H., O'Brien O. P. Action of an estrogen antagonist on reproductive mechanisms in the rat. Endocrinology. 1966 Jul;79(1):153–167. doi: 10.1210/endo-79-1-153. [DOI] [PubMed] [Google Scholar]
  5. Churg A., Anderson W. A. Induction of endometrial peroxidase synthesis and secretion by estrogen and estrogen antagonist. J Cell Biol. 1974 Aug;62(2):449–459. doi: 10.1083/jcb.62.2.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark J. H., Anderson J. N., Peck E. J., Jr Estrogen receptor-anti-estrogen complex: atypical binding by uterine nuclei and effects on uterine growth. Steroids. 1973 Nov;22(5):707–718. doi: 10.1016/0039-128x(73)90118-9. [DOI] [PubMed] [Google Scholar]
  7. Coval M. L., Taurog A. Purification and iodinating activity of hog thyroid peroxidase. J Biol Chem. 1967 Dec 10;242(23):5510–5523. [PubMed] [Google Scholar]
  8. DAO T. L. CARCINOGENESIS OF MAMMARY GLAND IN RAT. Prog Exp Tumor Res. 1964;5:157–216. doi: 10.1159/000385999. [DOI] [PubMed] [Google Scholar]
  9. DeAngelo A. B., Gorski J. Role of RNA synthesis in the estrogen induction of a specific uterine protein. Proc Natl Acad Sci U S A. 1970 Jul;66(3):693–700. doi: 10.1073/pnas.66.3.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HUGGINS C., BRIZIARELLI G., SUTTON H., Jr Rapid induction of mammary carcinoma in the rat and the influence of hormones on the tumors. J Exp Med. 1959 Jan 1;109(1):25–42. doi: 10.1084/jem.109.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hamilton T. H. Control by estrogen of genetic transcription and translation. Binding to chromatin and stimulation of nucleolar RNA synthesis are primary events in the early estrogen action. Science. 1968 Aug 16;161(3842):649–661. doi: 10.1126/science.161.3842.649. [DOI] [PubMed] [Google Scholar]
  12. Herzog V., Fahimi H. D. A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor. Anal Biochem. 1973 Oct;55(2):554–562. doi: 10.1016/0003-2697(73)90144-9. [DOI] [PubMed] [Google Scholar]
  13. Heuson J. C., Waelbroeck C., Legros N., Gallez G., Robyn C., L'Hermite M. Inhibition of DMBA-induced mammary carcinogenesis in the rat by 2-br- -ergocryptine (CB 154), an inhibitor of prolactin secretion, and by nafoxidine (U-11, 100 A), an estrogen antagonist. Gynecol Invest. 1971;2(1):130–137. doi: 10.1159/000301858. [DOI] [PubMed] [Google Scholar]
  14. Jellinck P. H., Fletcher R. Peroxidase-catalyzed conjugation of [4-14C]estradiol with albumin and thiols. Can J Biochem. 1970 Nov;48(11):1192–1198. doi: 10.1139/o70-186. [DOI] [PubMed] [Google Scholar]
  15. Jensen E. V., DeSombre E. R. Mechanism of action of the female sex hormones. Annu Rev Biochem. 1972;41:203–230. doi: 10.1146/annurev.bi.41.070172.001223. [DOI] [PubMed] [Google Scholar]
  16. Jensen E. V., Jacobson H. I., Smith S., Jungblut P. W., De Sombre E. R. The use of estrogen antagonists in hormone receptor studies. Gynecol Invest. 1972;3(1):108–123. doi: 10.1159/000301747. [DOI] [PubMed] [Google Scholar]
  17. KLEBANOFF S. J. INACTIVATION OF ESTROGEN BY RAT UTERINE PREPARATIONS. Endocrinology. 1965 Feb;76:301–311. doi: 10.1210/endo-76-2-301. [DOI] [PubMed] [Google Scholar]
  18. KLEBANOFF S. J., SEGAL S. J. The inactivation of estradiol by peroxidase. J Biol Chem. 1960 Jan;235:52–55. [PubMed] [Google Scholar]
  19. Klebanoff S. J., Clem W. H., Luebke R. G. The peroxidase-thiocyanate-hydrogen peroxide antimicrobial system. Biochim Biophys Acta. 1966 Mar 28;117(1):63–72. doi: 10.1016/0304-4165(66)90152-8. [DOI] [PubMed] [Google Scholar]
  20. Kohler P. O., Grimley P. M., O'Malley B. W. Estrogen-induced cytodifferentiation of the ovalbumin-secreting glands of the chick oviduct. J Cell Biol. 1969 Jan;40(1):8–27. doi: 10.1083/jcb.40.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  22. Lawton I. E., Schwartz N. B. Pituitary-ovarian function in rats exposed to constant light: a chronological study. Endocrinology. 1967 Sep;81(3):497–508. doi: 10.1210/endo-81-3-497. [DOI] [PubMed] [Google Scholar]
  23. MUELLER G. C., HERRANEN A. M., JERVELL K. F. Studies on the mechanism of action of estrogens. Recent Prog Horm Res. 1958;14:95–139. [PubMed] [Google Scholar]
  24. McGuire W. L., Julian J. A. Comparison of macromolecular binding of estradiol in hormone-dependent and hormone-independent rat mammary carcinoma. Cancer Res. 1971 Oct;31(10):1440–1445. [PubMed] [Google Scholar]
  25. O'Malley B. W., McGuire W. L., Kohler P. O., Korenman S. G. Studies on the mechanism of steroid hormone regulation of synthesis of specific proteins. Recent Prog Horm Res. 1969;25:105–160. doi: 10.1016/b978-0-12-571125-8.50006-5. [DOI] [PubMed] [Google Scholar]
  26. Peterson R. P., Spaziani E. Autoradiographic localization of albumin 131-I accumulation in the estrogen-stimulated rat uterus. Endocrinology. 1971 Nov;89(5):1280–1286. doi: 10.1210/endo-89-5-1280. [DOI] [PubMed] [Google Scholar]
  27. Rochefort H., Lignon F., Capony F. Formation of estrogen nuclear receptor in uterus: effect of androgens, estrone and nafoxidine. Biochem Biophys Res Commun. 1972 May 26;47(4):662–670. doi: 10.1016/0006-291x(72)90543-8. [DOI] [PubMed] [Google Scholar]
  28. Smith D. C., Klebanoff S. J. A uterine fluid-mediated sperm-inhibitory system. Biol Reprod. 1970 Oct;3(2):229–235. doi: 10.1093/biolreprod/3.2.229. [DOI] [PubMed] [Google Scholar]
  29. Strum J. M., Karnovsky M. J. Cytochemical localization of endogenous peroxidase in thyroid follicular cells. J Cell Biol. 1970 Mar;44(3):655–666. doi: 10.1083/jcb.44.3.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. WILLIAMS-ASHMAN H. G., JOHNSON M. K. Aromatic amines as hydrogen carriers for the peroxidase-catalyzed oxidation of reduced pyridine nucleotides. Enzymologia. 1960 Dec 31;22:327–331. [PubMed] [Google Scholar]
  31. Williams-Ashman H. G., Reddi A. H. Actions of vertebrate sex hormones. Annu Rev Physiol. 1971;33:31–82. doi: 10.1146/annurev.ph.33.030171.000335. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES