Abstract
The induction and localization of tyrocidine-synthesizing enzymes is shown to be parallel, during growth of Bacillus brevis (ATCC 8185, American Type Culture Collection, Rockville, Md.), with the induction of uptake of constitutive amino acids and of components of pantetheine, a coenzyme of tyrocidine synthesis. Antibiotic synthesis appears at the end of logarithmic growth when the first soluble enzymes may be obtained from homogenates. During this period, binding proteins for metabolite uptake were isolated by intensive sonication which, when studied by chromatography, were identified by the appearance of low molecular weight fractions binding the radioactively marked metabolites; their induction was prevented by addition of rifampicin. The major purpose of this study was a comparison of antibiotic production and sporulation, the progress of which was followed by electron microscopy. The onset of tyrocidine synthesis and metabolite uptake coincided with the appearance of septum formation indicating that sporulation had progressed to stage II. With the progress of spore encapsulation, the tyrocidine production migrated from the soluble fraction into the forespore, terminating with the separation of forespores from the sporangium membrane. The resulting concentration of antibiotic in the forespore may indicate its function in sporulation, the nature of which, however, was not explored.
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- Ames G. F., Lever J. Components of histidine transport: histidine-binding proteins and hisP protein. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1096–1103. doi: 10.1073/pnas.66.4.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anraku Y. The reduction and restoration of galactose transport in osmotically shocked cells of Escherichia coli. J Biol Chem. 1967 Mar 10;242(5):793–800. [PubMed] [Google Scholar]
- BERNLOHR R. W., NOVELLI G. D. BACITRACIN BIOSYNTHESIS AND SPORE FORMATION: THE PHYSIOLOGICAL ROLE OF AN ANTIBIOTIC. Arch Biochem Biophys. 1963 Oct;103:94–104. doi: 10.1016/0003-9861(63)90014-6. [DOI] [PubMed] [Google Scholar]
- Bauer K., Roskoski R., Jr, Kleinkauf H., Lipmann F. Synthesis of a linear gramicidin by a combination of biosynthetic and organic methods. Biochemistry. 1972 Aug 15;11(17):3266–3271. doi: 10.1021/bi00767a022. [DOI] [PubMed] [Google Scholar]
- Berger E. A., Heppel L. A. Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli. J Biol Chem. 1974 Dec 25;249(24):7747–7755. [PubMed] [Google Scholar]
- Boos W., Sarvas M. O. Close linkage between a galactose binding protein and the beta-methylgalactoside permease in Escherichia coli. Eur J Biochem. 1970 Apr;13(3):526–533. doi: 10.1111/j.1432-1033.1970.tb00956.x. [DOI] [PubMed] [Google Scholar]
- Fujikawa K., Suzuki T., Kurahashi K. Biosynthesis of tyrocidine by a cell-free enzyme system of Bacillus brevis ATCC 8185. I. Preparation of partially purified enzyme system and its properties. Biochim Biophys Acta. 1968 Jun 18;161(1):232–246. doi: 10.1016/0005-2787(68)90313-4. [DOI] [PubMed] [Google Scholar]
- Kambe M., Imae Y., Kurahashi K. Biochemical studies on gramicidin S non-producing mutants of Bacillus brevis ATCC 9999. J Biochem. 1974 Mar;75(3):481–493. doi: 10.1093/oxfordjournals.jbchem.a130417. [DOI] [PubMed] [Google Scholar]
- Lee S. G., Lipmann F. Tyrocidine synthetase system. Methods Enzymol. 1975;43:585–602. doi: 10.1016/0076-6879(75)43121-4. [DOI] [PubMed] [Google Scholar]
- Lee S. G., Roskoski R., Jr, Bauer K., Lipmann F. Purification of the polyenzymes responsible for tyrocidine synthesis and their dissociation into subunits. Biochemistry. 1973 Jan 30;12(3):398–405. doi: 10.1021/bi00727a006. [DOI] [PubMed] [Google Scholar]
- Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
- Pardee A. B., Prestidge L. S., Whipple M. B., Dreyfuss J. A binding site for sulfate and its relation to sulfate transport into Salmonella typhimurium. J Biol Chem. 1966 Sep 10;241(17):3962–3969. [PubMed] [Google Scholar]
- Piperno J. R., Oxender D. L. Amino-acid-binding protein released from Escherichia coli by osmotic shock. J Biol Chem. 1966 Dec 10;241(23):5732–5734. [PubMed] [Google Scholar]
- Roskoski R., Jr, Gevers W., Kleinkauf H., Lipmann F. Tyrocidine biosynthesis by three complementary fractions from Bacillus brevis (ATCC 8185). Biochemistry. 1970 Dec 8;9(25):4839–4845. doi: 10.1021/bi00827a002. [DOI] [PubMed] [Google Scholar]
- Roskoski R., Jr, Kleinkauf H., Gevers W., Lipmann F. Isolation of enzyme-bound peptide intermediates in tyrocidine biosynthesis. Biochemistry. 1970 Dec 8;9(25):4846–4851. doi: 10.1021/bi00827a003. [DOI] [PubMed] [Google Scholar]
- Sarkar N., Paulus H. Function of peptide antibiotics in sporulation. Nat New Biol. 1972 Oct 25;239(95):228–230. doi: 10.1038/newbio239228a0. [DOI] [PubMed] [Google Scholar]
- Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]