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. 1975 Nov 1;67(2):469–476. doi: 10.1083/jcb.67.2.469

Intercellular communication in the rat anterior pituitary gland: an in vivo and in vitro study

WH Fletcher, NC Anderson, JW Everett
PMCID: PMC2109609  PMID: 1194357

Abstract

The concept of "stimulus-secretion coupling" suggested by Douglas and co-workers to explain the events related to monamine discharge by the adrenal medulla (5, 7) may be applied to other endocrine tissues, such as adrenal cortex (36), pancreatic islets (4), and magnocellular hypothalamic neurons (6), which exhibit a similar ion-dependent process of hormone elaboration. In addition, they share another feature, that of joining neighbor cells via membrane junctions (12, 26, and Fletcher, unpublished observation). Given this, and the reports that hormone secretion by the pars distalis also involves a secretagogue-induced decrease in membrane bioelectric potential accompanied by a rise in cellular [Ca++] (27, 34, 41), it was appropriate to test the possibility that cells of the anterior pituitary gland are united by junctions.

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Selected References

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  1. Bennett M. V. Function of electrotonic junctions in embryonic and adult tissues. Fed Proc. 1973 Jan;32(1):65–75. [PubMed] [Google Scholar]
  2. Berry M. N., Friend D. S. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969 Dec;43(3):506–520. doi: 10.1083/jcb.43.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Branton D. Fracture faces of frozen membranes. Proc Natl Acad Sci U S A. 1966 May;55(5):1048–1056. doi: 10.1073/pnas.55.5.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DOUGLAS W. W., POISNER A. M. On the mode of action of acetylcholine in evoking adrenal medullary secretion: increased uptake of calcium during the secretory response. J Physiol. 1962 Aug;162:385–392. doi: 10.1113/jphysiol.1962.sp006940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DOUGLAS W. W., POISNER A. M. STIMULUS-SECRETION COUPLING IN A NEUROSECRETORY ORGAN: THE ROLE OF CALCIUM IN THE RELEASE OF VASOPRESSIN FROM THE NEUROHYPOPHYSIS. J Physiol. 1964 Jul;172:1–18. doi: 10.1113/jphysiol.1964.sp007399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DOUGLAS W. W., RUBIN R. P. The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J Physiol. 1961 Nov;159:40–57. doi: 10.1113/jphysiol.1961.sp006791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dean P. M., Matthews E. K. Electrical activity in pancreatic islet cells. Nature. 1968 Jul 27;219(5152):389–390. doi: 10.1038/219389a0. [DOI] [PubMed] [Google Scholar]
  8. FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FAWCETT D. W., ITO S., SLAUTTERBACK D. The occurrence of intercellular bridges in groups of cells exhibiting synchronous differentiation. J Biophys Biochem Cytol. 1959 May 25;5(3):453–460. doi: 10.1083/jcb.5.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Friend D. S., Gilula N. B. A distinctive cell contact in the rat adrenal cortex. J Cell Biol. 1972 Apr;53(1):148–163. doi: 10.1083/jcb.53.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gilula N. B., Reeves O. R., Steinbach A. Metabolic coupling, ionic coupling and cell contacts. Nature. 1972 Feb 4;235(5336):262–265. doi: 10.1038/235262a0. [DOI] [PubMed] [Google Scholar]
  12. Herlant M. The cells of the adenohypophysis and their functional significance. Int Rev Cytol. 1964;17:299–382. doi: 10.1016/s0074-7696(08)60409-x. [DOI] [PubMed] [Google Scholar]
  13. Hopkins C. R., Farquhar M. G. Hormone secretion by cells dissociated from rat anterior pituitaries. J Cell Biol. 1973 Nov;59(2 Pt 1):276–303. doi: 10.1083/jcb.59.2.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hymer W. C., Kraicer J., Bencosme S. A., Haskill J. S. Separation of somatotrophs from the rat adenohypophysis by velocity and density gradient centrifugation. Proc Soc Exp Biol Med. 1972 Dec;141(3):966–973. doi: 10.3181/00379727-141-36912. [DOI] [PubMed] [Google Scholar]
  15. Livingston R. B., Pfenniger K., Moor H., Akert K. Specialized paranodal and interparanodal glial-axonal junctions in the peripheral and central nervous system: a freeze-etching study. Brain Res. 1973 Aug 17;58(1):1–24. doi: 10.1016/0006-8993(73)90820-2. [DOI] [PubMed] [Google Scholar]
  16. Luft J. H. Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat Rec. 1971 Nov;171(3):369–415. doi: 10.1002/ar.1091710303. [DOI] [PubMed] [Google Scholar]
  17. Orci L., Like A. A., Amherdt M., Blondel B., Kanazawa Y., Marliss E. B., Lambert A. E., Wollheim C. B., Renold A. E. Monolayer cell culture of neonatal rat pancreas: an ultrastructural and biochemical study of functioning endocrine cells. J Ultrastruct Res. 1973 May;43(3):270–297. doi: 10.1016/s0022-5320(73)80039-5. [DOI] [PubMed] [Google Scholar]
  18. Peracchia C. Low resistance junctions in crayfish. I. Two arrays of globules in junctional membranes. J Cell Biol. 1973 Apr;57(1):66–76. doi: 10.1083/jcb.57.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pinto da Silva P., Branton D. Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker. J Cell Biol. 1970 Jun;45(3):598–605. doi: 10.1083/jcb.45.3.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. ROBERTSON J. D. THE OCCURRENCE OF A SUBUNIT PATTERN IN THE UNIT MEMBRANES OF CLUB ENDINGS IN MAUTHNER CELL SYNAPSES IN GOLDFISH BRAINS. J Cell Biol. 1963 Oct;19:201–221. doi: 10.1083/jcb.19.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rubin R. P., Jaanus S. D., Carchman R. A., Puig M. Reversible inhibition of ACTH-induced corticosteroid release by cycloheximide: evidence for an unidentified cellular messenger. Endocrinology. 1973 Sep;93(3):575–580. doi: 10.1210/endo-93-3-575. [DOI] [PubMed] [Google Scholar]
  22. Samli M. H., Geschwind I. I. Some effects of energy-transfer inhibitors and of Ca++-free or K+-enhanced media on the release of luteinizing hormone (LH) from the rat pituitary gland in vitro. Endocrinology. 1968 Feb;82(2):225–231. doi: 10.1210/endo-82-2-225. [DOI] [PubMed] [Google Scholar]
  23. Satir P., Gilula N. B. The fine structure of membranes and intercellular communication in insects. Annu Rev Entomol. 1973;18:143–166. doi: 10.1146/annurev.en.18.010173.001043. [DOI] [PubMed] [Google Scholar]
  24. Sheridan J. D. Electrical coupling between fat cells in newt fat body and mouse brown fat. J Cell Biol. 1971 Sep;50(3):795–803. doi: 10.1083/jcb.50.3.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Subak-Sharpe H., Bürk R. R., Pitts J. D. Metabolic co-operation between biochemically marked mammalian cells in tissue culture. J Cell Sci. 1969 Mar;4(2):353–367. doi: 10.1242/jcs.4.2.353. [DOI] [PubMed] [Google Scholar]
  26. York D. H., Baker F. L., Kraicer J. Electrical changes induced in rat adenohypophysial cells, in vivo, with hypothalamic extract. Neuroendocrinology. 1973;11(4):212–228. doi: 10.1159/000122134. [DOI] [PubMed] [Google Scholar]

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