Abstract
Several and various types of cells contain fine cytoplasmic filaments closely resembling the myofilaments of muscle cells (2, 18, 23, 24). In many of these cells and especially when cultured, it has been demonstrated that some of these filaments react with heavy meromyosin (HMM) in the same way as do the actin filaments of muscle cells (3, 6 7). This suggests that these filaments may be actinoid and form part of a contractile system. As fine intracytoplasmic filaments do occur in lymphatic endothelial cells (2, 14), we undertook an electron microscope investigation of their fine structure and their reaction on incubation with HMM and EDTA. We postulated that lymphatic endothelial cells possess a contractile filamentous system to which these filaments belong.
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Selected References
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- Allison A. C., Davies P., De Petris S. Role of contractile microfilaments in macrophage movement and endocytosis. Nat New Biol. 1971 Aug 4;232(31):153–155. doi: 10.1038/newbio232153a0. [DOI] [PubMed] [Google Scholar]
- Burton P. R., Kirkland W. L. Actin detected in mouse neuroblastoma cells by binding of heavy meromyosin. Nat New Biol. 1972 Oct 25;239(95):244–246. doi: 10.1038/newbio239244a0. [DOI] [PubMed] [Google Scholar]
- Chang C. M., Goldman R. D. The localization of actin-like fibers in cultured neuroblastoma cells as revealed by heavy meromyosin binding. J Cell Biol. 1973 Jun;57(3):867–874. doi: 10.1083/jcb.57.3.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooke P. H., Fay F. S. Correlation between fiber length, ultrastructure, and the length-tension relationship of mammalian smooth muscle. J Cell Biol. 1972 Jan;52(1):105–116. doi: 10.1083/jcb.52.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRAENICHER D., PORTZEHL H. THE INFLUENCE OF MAGNESIUM AND CALCIUM PYROPHOSPHATE CHELATES, OF FREE MAGNESIUM IONS, FREE CALCIUM IONS, AND FREE PYROPHOSPHATE IONS ON THE DISSOCIATION OF ACTYMYOSIN IN SOLUTION. Biochim Biophys Acta. 1964 Jun 8;86:567–578. doi: 10.1016/0304-4165(64)90096-0. [DOI] [PubMed] [Google Scholar]
- HUXLEY H. E. ELECTRON MICROSCOPE STUDIES ON THE STRUCTURE OF NATURAL AND SYNTHETIC PROTEIN FILAMENTS FROM STRIATED MUSCLE. J Mol Biol. 1963 Sep;7:281–308. doi: 10.1016/s0022-2836(63)80008-x. [DOI] [PubMed] [Google Scholar]
- Ishikawa H., Bischoff R., Holtzer H. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol. 1969 Nov;43(2):312–328. [PMC free article] [PubMed] [Google Scholar]
- Kristensen B. I., Nielsen L. E. A two-filament system and interaction of heavy meromyosin (HMM) with thin filaments in smooth muscle. Z Zellforsch Mikrosk Anat. 1971;122(3):350–356. doi: 10.1007/BF00935994. [DOI] [PubMed] [Google Scholar]
- Kristensen B. I., Simonsen L. O., Pape L. Actin-like filaments in Ehrlich ascites tumor cells and their reaction with heavy meromyosin. Virchows Arch B Cell Pathol. 1973 Jun 25;13(2):103–112. doi: 10.1007/BF02889301. [DOI] [PubMed] [Google Scholar]
- LOWEY S., COHEN C. Studies on the structure of myosin. J Mol Biol. 1962 Apr;4:293–308. doi: 10.1016/s0022-2836(62)80007-2. [DOI] [PubMed] [Google Scholar]
- Lauweryns J. M., Baert J. H. The role of the pulmonary lymphatics in the defenses of the distal lung: morphological and experimental studies of the transport mechanisms of intratracheally instillated particles. Ann N Y Acad Sci. 1974;221:244–275. doi: 10.1111/j.1749-6632.1974.tb28225.x. [DOI] [PubMed] [Google Scholar]
- Lauweryns J. M., Boussauw L. The ultrastructure of lymphatic valves in the adult rabbit lung. Z Zellforsch Mikrosk Anat. 1973;143(2):149–168. doi: 10.1007/BF00307476. [DOI] [PubMed] [Google Scholar]
- Lauweryns J. M., Boussauw L. The ultrastructure of pulmonary lymphatic capillaries of newborn rabbits and of human infants. Lymphology. 1969 Sep;2(3):108–129. [PubMed] [Google Scholar]
- Leak L. V., Burke J. F. Fine structure of the lymphatic capillary and the adjoining connective tissue area. Am J Anat. 1966 May;118(3):785–809. doi: 10.1002/aja.1001180308. [DOI] [PubMed] [Google Scholar]
- Leak L. V. Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue-lymph interface. Microvasc Res. 1970 Oct;2(4):361–391. doi: 10.1016/0026-2862(70)90031-2. [DOI] [PubMed] [Google Scholar]
- Orci L., Gabbay K. H., Malaisse W. J. Pancreatic beta-cell web: its possible role in insulin secretion. Science. 1972 Mar 10;175(4026):1128–1130. doi: 10.1126/science.175.4026.1128. [DOI] [PubMed] [Google Scholar]
- Panner B. J., Honig C. R. Filament ultrastructure and organization in vertebrate smooth muscle. Contraction hypothesis based on localization of actin and myosin. J Cell Biol. 1967 Nov;35(2):303–321. doi: 10.1083/jcb.35.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phelps P. C., Luft J. H. Electron microscopical study of relaxation and constriction in frog arterioles. Am J Anat. 1969 Aug;125(4):399–427. doi: 10.1002/aja.1001250404. [DOI] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Röhlich P., Oláh I. Cross-striated fibrils in the endothelium of the rat myometral arterioles. J Ultrastruct Res. 1967 Jun;18(5):667–676. doi: 10.1016/s0022-5320(67)80212-0. [DOI] [PubMed] [Google Scholar]
- Vajda J., Tomcsik M. The structure of the valves of the lymphatic vessels. Acta Anat (Basel) 1971;78(4):521–531. doi: 10.1159/000143611. [DOI] [PubMed] [Google Scholar]