Abstract
Glycine, an inhibitory transmitter in spinal cord, is taken up into specific nerve terminals by means of a unique high-affinity uptake system. In this study, [3H]glycine was directly microinjected into rat ventral horn in vivo and electron microscope autoradiography used to localize the label in various anatomic compartments. Quantiative analysis showed that [3H]glycine labeled a high proportion of axosomatic and axodendritic synapses which presumably act to inhibit spinal motor neurons.
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Selected References
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- Arregui A., Logan W. J., Bennett J. P., Snyder S. H. Specific glycine--accumulating synaptosomes in the spinal cord of rats. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3485–3489. doi: 10.1073/pnas.69.11.3485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burke R. E., Fedina L., Lundberg A. Spatial synaptic distribution of recurrent and group Ia inhibitory systems in cat spinal motoneurones. J Physiol. 1971 Apr;214(2):305–326. doi: 10.1113/jphysiol.1971.sp009434. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CURTIS D. R., ECCLES J. C. The time courses of excitatory and inhibitory synaptic actions. J Physiol. 1959 Mar 12;145(3):529–546. doi: 10.1113/jphysiol.1959.sp006159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curtis D. R., Hösli L., Johnston G. A., Johnston I. H. The hyperpolarization of spinal motoneurones by glycine and related amino acids. Exp Brain Res. 1968;5(3):235–258. doi: 10.1007/BF00238666. [DOI] [PubMed] [Google Scholar]
- Davidoff R. A., Shank R. P., Graham L. T., Jr, Aprison M. H., Werman R. Association of glycine with spinal interneurones. Nature. 1967 May 13;214(5089):680–681. doi: 10.1038/214680a0. [DOI] [PubMed] [Google Scholar]
- Faeder I. R., Salpeter M. M. Glutamate uptake by a stimulated insect nerve muscle preparation. J Cell Biol. 1970 Aug;46(2):300–307. doi: 10.1083/jcb.46.2.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hökfelt T., Ljungdahl A. Light and electron microscopic autoradiography on spinal cord slices after incubation with labeled glycine. Brain Res. 1971 Sep 10;32(1):189–194. doi: 10.1016/0006-8993(71)90163-6. [DOI] [PubMed] [Google Scholar]
- Iversen L. L., Bloom F. E. Studies of the uptake of 3 H-gaba and ( 3 H)glycine in slices and homogenates of rat brain and spinal cord by electron microscopic autoradiography. Brain Res. 1972 Jun 8;41(1):131–143. doi: 10.1016/0006-8993(72)90621-x. [DOI] [PubMed] [Google Scholar]
- Jordan C. C., Webster R. A. Release of acetylcholine and 14 C-glycine from the cat spinal cord in vivo. Br J Pharmacol. 1971 Oct;43(2):441P–441P. [PMC free article] [PubMed] [Google Scholar]
- Ljungdahl A., Hökfelt T. Autoradiographic uptake patterns of (3H)GABA and (3H)glycine in central nervous tissues with special reference to the cat spinal cord. Brain Res. 1973 Nov 23;62(2):587–595. doi: 10.1016/0006-8993(73)90726-9. [DOI] [PubMed] [Google Scholar]
- Matus A. I., Dennison M. E. An autoradiographic study of uptake of exogenous glycine by vertebrate spinal cord splices in vitro. J Neurocytol. 1972 Jul;1(1):27–34. doi: 10.1007/BF01098643. [DOI] [PubMed] [Google Scholar]
- Neal M. J., Pickles H. G. Uptake of 14C glycine by spinal cord. Nature. 1969 May 17;222(5194):679–680. doi: 10.1038/222679a0. [DOI] [PubMed] [Google Scholar]
- Neal M. J. The uptake of [14C]glycine by slices of mammalian spinal cord. J Physiol. 1971 May;215(1):103–117. doi: 10.1113/jphysiol.1971.sp009460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orkand P. M., Kravitz E. A. Localization of the sites of gamma-aminobutyric acid (GABA) uptake in lobster nerve-muscle preparations. J Cell Biol. 1971 Apr;49(1):75–89. doi: 10.1083/jcb.49.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peters T., Jr, Ashley C. A. An artefact in radioautography due to binding of free amino acids to tissues by fixatives. J Cell Biol. 1967 Apr;33(1):53–60. doi: 10.1083/jcb.33.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snyder S. H., Young A. B., Bennett J. P., Mulder A. H. Synaptic biochemistry of amino acids. Fed Proc. 1973 Oct;32(10):2039–2047. [PubMed] [Google Scholar]
- Werman R. Amino acids as central neurotransmitters. Res Publ Assoc Res Nerv Ment Dis. 1972;50:147–180. [PubMed] [Google Scholar]
- Werman R., Davidoff R. A., Aprison M. H. Inhibitory of glycine on spinal neurons in the cat. J Neurophysiol. 1968 Jan;31(1):81–95. doi: 10.1152/jn.1968.31.1.81. [DOI] [PubMed] [Google Scholar]