Abstract
Spread chromatin fibers and isolated chromatin fragments prepared from chicken erythrocyte nuclei were stained with dilute aqueous uranyl acetate. High-resolution electron micrographs reveal two new morphological features exhibited by many of the chromatin nu bodies: (a) lateral association of the nu body with the connecting strand, and (b) a centrally stained spot approximately 15 A wide, possibly corresponding to a hole or crevice within the nu body.
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- Baldwin J. P., Boseley P. G., Bradbury E. M., Ibel K. The subunit structure of the eukaryotic chromosome. Nature. 1975 Jan 24;253(5489):245–249. doi: 10.1038/253245a0. [DOI] [PubMed] [Google Scholar]
- Bram S., Ris H. On the structure of nucleohistone. J Mol Biol. 1971 Feb 14;55(3):325–336. doi: 10.1016/0022-2836(71)90321-4. [DOI] [PubMed] [Google Scholar]
- D'Anna J. A., Jr, Isenberg I. A complex of histones IIb2 and IV. Biochemistry. 1973 Mar 13;12(6):1035–1043. doi: 10.1021/bi00730a003. [DOI] [PubMed] [Google Scholar]
- D'Anna J. A., Jr, Isenberg I. A histone cross-complexing pattern. Biochemistry. 1974 Nov 19;13(24):4992–4997. doi: 10.1021/bi00721a019. [DOI] [PubMed] [Google Scholar]
- D'Anna J. A., Jr, Isenberg I. Interactions of histone LAK (f2a2) with histones KAS (f2b) and GRK (f2a1). Biochemistry. 1974 May 7;13(10):2098–2104. doi: 10.1021/bi00707a016. [DOI] [PubMed] [Google Scholar]
- Hewish D. R., Burgoyne L. A. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973 May 15;52(2):504–510. doi: 10.1016/0006-291x(73)90740-7. [DOI] [PubMed] [Google Scholar]
- Kelley R. I. Isolation of a histone IIb1-IIb2 complex. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1588–1594. doi: 10.1016/0006-291x(73)91168-6. [DOI] [PubMed] [Google Scholar]
- Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
- Kornberg R. D., Thomas J. O. Chromatin structure; oligomers of the histones. Science. 1974 May 24;184(4139):865–868. doi: 10.1126/science.184.4139.865. [DOI] [PubMed] [Google Scholar]
- Martinson H. G., McCarthy B. J. Histone-histone associations within chromatin. Cross-linking studies using tetranitromethane. Biochemistry. 1975 Mar 11;14(5):1073–1078. doi: 10.1021/bi00676a030. [DOI] [PubMed] [Google Scholar]
- Noll M. Subunit structure of chromatin. Nature. 1974 Sep 20;251(5472):249–251. doi: 10.1038/251249a0. [DOI] [PubMed] [Google Scholar]
- Olins A. L., Carlson R. D., Olins D. E. Visualization of chromatin substructure: upsilon bodies. J Cell Biol. 1975 Mar;64(3):528–537. doi: 10.1083/jcb.64.3.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olins A. L., Olins D. E. Spheroid chromatin units (v bodies). Science. 1974 Jan 25;183(4122):330–332. doi: 10.1126/science.183.4122.330. [DOI] [PubMed] [Google Scholar]
- Olins D. E., Wright E. B. Glutaraldehyde fixation of isolated eucaryotic nuclei. Evidence for histone-histone proximity. J Cell Biol. 1973 Nov;59(2 Pt 1):304–317. doi: 10.1083/jcb.59.2.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oosterhof D. K., Hozier J. C., Rill R. L. Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils. Proc Natl Acad Sci U S A. 1975 Feb;72(2):633–637. doi: 10.1073/pnas.72.2.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oudet P., Gross-Bellard M., Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. doi: 10.1016/0092-8674(75)90149-x. [DOI] [PubMed] [Google Scholar]
- Pardon J. F., Wilkins M. H. A super-coil model for nucleohistone. J Mol Biol. 1972 Jul 14;68(1):115–124. doi: 10.1016/0022-2836(72)90267-7. [DOI] [PubMed] [Google Scholar]
- Pardon J. F., Wilkins M. H., Richards B. M. Super-helical model for nucleohistone. Nature. 1967 Jul 29;215(5100):508–509. doi: 10.1038/215508a0. [DOI] [PubMed] [Google Scholar]
- Rill R., Van Holde K. E. Properties of nuclease-resistant fragments of calf thymus chromatin. J Biol Chem. 1973 Feb 10;248(3):1080–1083. [PubMed] [Google Scholar]
- Senior M. B., Olins A. L., Olins D. E. Chromatin fragments resembling v bodies. Science. 1975 Jan 17;187(4172):173–175. doi: 10.1126/science.1111096. [DOI] [PubMed] [Google Scholar]
- Skandrani E., Mizon J., Sautière P., Biserte G. Etude de la fraction F2b des histones de thymus de veau. Biochimie. 1972;54(10):1267–1272. doi: 10.1016/s0300-9084(72)80067-1. [DOI] [PubMed] [Google Scholar]
- Van Lente F., Jackson J. F., Weintraub H. Identification of specific crosslinked histones after treatment of chromatin with formaldehyde. Cell. 1975 May;5(1):45–50. doi: 10.1016/0092-8674(75)90090-2. [DOI] [PubMed] [Google Scholar]
- Weintraub H. Release of discrete subunits after nuclease and trypsin digestion of chromatin. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1212–1216. doi: 10.1073/pnas.72.3.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weintraub H., Van Lente F. Dissection of chromosome structure with trypsin and nucleases. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4249–4253. doi: 10.1073/pnas.71.10.4249. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZOBEL C. R., BEER M. Electron stains. I. Chemical studies on the interaction of DNA with uranyl salts. J Biophys Biochem Cytol. 1961 Jul;10:335–346. doi: 10.1083/jcb.10.3.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Bruggen E. F., Arnberg A. C., van Holde K. E., Sahasrabuddhe C. G., Shaw B. R. Electron microscopy of chromatin subunit particles. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1365–1370. doi: 10.1016/0006-291x(74)90348-9. [DOI] [PubMed] [Google Scholar]