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. 1976 May 1;69(2):415–428. doi: 10.1083/jcb.69.2.415

Asymmetry of spermiation and sperm surface charge patterns over the giant acrosome in the musk shrew Suncus murinus

PMCID: PMC2109686  PMID: 1262397

Abstract

Spermatozoa of the shrew Suncus murinus, a mammal with abdominal testes, exhibit four unusual features: a giant acrosome; a dorsoventral asymmetry of their spermiation; a dorsoventral asymmetry of their head surface character; and also apparent surface maturity as they enter the epididymis. A Sertoli cell-periacrosomal cisternal complex envelops the giant acrosome during spermatid maturation. Spermiation is heraled by asymmetrical disorganization of the subplasmalemmal components of this complex and is completed by retraction of the Sertoli cell from the ventral and then the dorsal face of the spermatid head. This sequence or release is correlated with an asynchronous acquisition of negative surface charges on the spermatid head-demonstrable on glutaraldehyde- stabilized cells by the binding at pH 1.8 of positively charged colloidal particles of ferric oxide. Mature epididymal spermatozoa exhibit an asymmetry in the patterns of distribution of bound colloid over the dorsal vs. ventral surfaces of the sperm head, as well as regional differences between the tail midpiece and principal piece. Surface distributions of anionic residues and lectin (Con A)-binding sites characteristic of mature Suncus spermatozoa are demonstrable within the testis, unlike the situation in most nannals where distinct modifications of the sperm surface occur during epididymal passage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen G. J., Bishop M. W., Thompson T. E. Lysis of photographic emulsions by mammalian and chicken spermatozoa. J Reprod Fertil. 1974 Jan;36(1):249–252. doi: 10.1530/jrf.0.0360249. [DOI] [PubMed] [Google Scholar]
  2. BEDFORD J. M. CHANGES IN THE ELECTROPHORETIC PROPERTIES OF RABBIT SPERMATOZOA DURING PASSAGE THROUGH THE EPIDIDYMIS. Nature. 1963 Dec 21;200:1178–1180. doi: 10.1038/2001178a0. [DOI] [PubMed] [Google Scholar]
  3. Bedford J. M., Calvin H., Cooper G. W. The maturation of spermatozoa in the human epididymis. J Reprod Fertil Suppl. 1973 Jul;18:199–213. [PubMed] [Google Scholar]
  4. Bedford J. M. Mechanisms involved in penetration of spermatozoa through the vestments of the mammalian egg. Basic Life Sci. 1974;4(Pt B):55–68. doi: 10.1007/978-1-4684-2892-6_4. [DOI] [PubMed] [Google Scholar]
  5. Bedford J. M., Nicander L. Ultrastructural changes in the acrosome and sperm membranes during maturation of spermatozoa in the testis and epididymis of the rabbit and monkey. J Anat. 1971 Apr;108(Pt 3):527–543. [PMC free article] [PubMed] [Google Scholar]
  6. Calvin H. I., Bedford J. M. Formation of disulphide bonds in the nucleus and accessory structures of mammalian spermatozoa during maturation in the epididymis. J Reprod Fertil Suppl. 1971 May;13(Suppl):65–75. [PubMed] [Google Scholar]
  7. Cooper G. W., Bedford J. M. Charge density change in the vitelline surface following fertilization of the rabbit egg. J Reprod Fertil. 1971 Jun;25(3):431–436. doi: 10.1530/jrf.0.0250431. [DOI] [PubMed] [Google Scholar]
  8. Dryden G. L., McAllister H. Y. Sustained fertility after CdCl 2 injection by a non-scrotal mammal, Suncus murinus (Insectivora, Soricidae). Biol Reprod. 1970 Aug;3(1):23–30. doi: 10.1093/biolreprod/3.1.23. [DOI] [PubMed] [Google Scholar]
  9. Edelman G. M., Millette C. F. Molecular probes of spermatozoan structures. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2436–2440. doi: 10.1073/pnas.68.10.2436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fawcett D. W. The mammalian spermatozoon. Dev Biol. 1975 Jun;44(2):394–436. doi: 10.1016/0012-1606(75)90411-x. [DOI] [PubMed] [Google Scholar]
  11. Fellous M., Gachelin G., Buc-Caron M. H., Dubois P., Jacob F. Similar location of an early embryonic antigen on mouse and human spermatozoa. Dev Biol. 1974 Dec;41(2):331–337. doi: 10.1016/0012-1606(74)90310-8. [DOI] [PubMed] [Google Scholar]
  12. Flickinger C., Fawcett D. W. The junctional specializations of Sertoli cells in the seminiferous epithelium. Anat Rec. 1967 Jun;158(2):207–221. doi: 10.1002/ar.1091580210. [DOI] [PubMed] [Google Scholar]
  13. Gasic G. J., Berwick L., Sorrentino M. Positive and negative colloidal iron as cell surface electron stains. Lab Invest. 1968 Jan;18(1):63–71. [PubMed] [Google Scholar]
  14. Hjort T., Hansen K. B. Immunofluorescent studies on human spermatozoa. I. The detection of different spermatozoal antibodies and their occurrence in normal and infertile women. Clin Exp Immunol. 1971 Jan;8(1):9–23. [PMC free article] [PubMed] [Google Scholar]
  15. Husted S. Sperm antibodies in men from infertile couples. Analysis of sperm agglutinins and immunofluorescent antibodies in 657 men. Int J Fertil. 1975;20(2):113–121. [PubMed] [Google Scholar]
  16. KARLSSON U., SCHULTZ R. L. FIXATION OF THE CENTRAL NERVOUS SYSTEM FROM ELECTRON MICROSCOPY BY ALDEHYDE PERFUSION. I. PRESERVATION WITH ALDEHYDE PERFUSATES VERSUS DIRECT PERFUSION WITH OSMIUM TETROXIDE WITH SPECIAL REFERENCE TO MEMBRANES AND THE EXTRACELLULAR SPACE. J Ultrastruct Res. 1965 Feb;12:160–186. doi: 10.1016/s0022-5320(65)80014-4. [DOI] [PubMed] [Google Scholar]
  17. Koo G. C., Stackpole C. W., Boyse E. A., Hämmerling U., Lardis M. P. Topographical location of H-Y antigen on mouse spermatozoa by immunoelectronmicroscopy. Proc Natl Acad Sci U S A. 1973 May;70(5):1502–1505. doi: 10.1073/pnas.70.5.1502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nicolson G. L., Yanagimachi R. Terminal saccharides on sperm plasma membranes: identification by specific agglutinins. Science. 1972 Jul 21;177(4045):276–279. doi: 10.1126/science.177.4045.276. [DOI] [PubMed] [Google Scholar]
  19. Vitale-Calpe R., Burgos M. H. The mechanism of spermiation in the hamster. I. Ultrastructure of spontaneous spermiation. J Ultrastruct Res. 1970 Jun;31(5-6):381–393. doi: 10.1016/s0022-5320(70)90156-5. [DOI] [PubMed] [Google Scholar]
  20. Vitale-Calpe R., Burgos M. H. The mechanism of spermiation in the hamster. II. The ultrastructural effects of coitus and of LH administration. J Ultrastruct Res. 1970 Jun;31(5-6):394–406. doi: 10.1016/s0022-5320(70)90157-7. [DOI] [PubMed] [Google Scholar]
  21. Yanagimachi R., Noda Y. D., Fujimoto M., Nicolson G. L. The distribution of negative surface charges on mammalian spermatozoa. Am J Anat. 1972 Dec;135(4):497–519. doi: 10.1002/aja.1001350405. [DOI] [PubMed] [Google Scholar]

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