Abstract
Cultured callus tissue of hazel (Corylus avellana L.), which has the potency of somatic embryogenesis, was used for the study of cell ultrastructure in the course of callus growth and embryoid formation. The meristematic cells of this tissue exhibit a specific organization of rough endoplasmic reticulum (RER), stacked into extensive parallel sheets. The membranes of the aggregated RER are associated with orderly arrays of bound ribosomes. The high regularity of the alignment of the attached ribosomes seems to be influenced by the distance between the two neighbouring membranes in the RER aggregate. The RER aggregates with orderly attached ribosomes are more frequently found in callus cells and in early embryogenesis than in the advanced stages of embryo development.
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Selected References
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- Byers B. Structure and formation of ribosome crystals in hypothermic chick embryo cells. J Mol Biol. 1967 Jun 14;26(2):155–167. doi: 10.1016/0022-2836(67)90288-4. [DOI] [PubMed] [Google Scholar]
- Duckett J. G. Pentagonal arrays of ribosomes in fertilized eggs of Pteridium aquilinum (L.) Kuhn. J Ultrastruct Res. 1972 Feb;38(3):390–397. doi: 10.1016/s0022-5320(72)90013-5. [DOI] [PubMed] [Google Scholar]
- ECHLIN P. AN APPARENT HELICAL ARRANGEMENT OF RIBOSOMES IN DEVELOPING POLLEN MOTHER CELLS OF IPOMOEA PURPUREA (L.) ROTH. J Cell Biol. 1965 Jan;24:150–153. doi: 10.1083/jcb.24.1.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ITO S., WINCHESTER R. J. The fine structure of the gastric mucosa in the bat. J Cell Biol. 1963 Mar;16:541–577. doi: 10.1083/jcb.16.3.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jensen W. A. Cotton embryogenesis. Polysome formation in the zygote. J Cell Biol. 1968 Feb;36(2):403–406. doi: 10.1083/jcb.36.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kingsbury E. W., Voelz H. Induction of helical arrays of ribosomes by vinblastine sulfate in Escherichia coli. Science. 1969 Nov 7;166(3906):768–769. doi: 10.1126/science.166.3906.768. [DOI] [PubMed] [Google Scholar]
- Morimoto T., Blobel G., Sabatini D. D. Ribosome crystallization in chicken embryos. I. Isolation, characterization, and in vitro activity of ribosome tetramers. J Cell Biol. 1972 Feb;52(2):338–354. doi: 10.1083/jcb.52.2.338. [DOI] [PMC free article] [PubMed] [Google Scholar]