Abstract
The ability of anionic groups on the luminal surface of blood vessels to redistribute by lateral migration under the influence of multivalent ligands was analyzed by electron microscopy, using cationized ferritin (CF). In vitro interaction of blood vessel segments with CF results in rapid aggregation of most anionic sites on the luminal fromt of the endothelium, followed by internalization or detachment of the CF patches, leaving most of the luminal surface devoid of anionic sites. Further incubation of such endothelial cells without CF results in regeneration of binding capacity for the polycationic label. Transport of CF, but not of native ferritin, across the endothelium by vesicle transport, followed by exocytosis of the interiorized CF clusters on the tissue front of the endothelium, was also observed. The possibility that such activities in the blood vessels in vivo may be associated with local changes in the normal distribution of the surface anionic sites as well as in accumulation of debris in the subendothelial layers of the vessels is suggested.
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- Clementi F., Palade G. E. Intestinal capillaries. I. Permeability to peroxidase and ferritin. J Cell Biol. 1969 Apr;41(1):33–58. doi: 10.1083/jcb.41.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Danon D., Goldstein L., Marikovsky Y., Skutelsky E. Use of cationized ferritin as a label of negative charges on cell surfaces. J Ultrastruct Res. 1972 Mar;38(5):500–510. doi: 10.1016/0022-5320(72)90087-1. [DOI] [PubMed] [Google Scholar]
- Joris I., Majno G. Cellular breakdown within the arterial wall. An ultrastructural study of the coronary artery in young and aging rats. Virchows Arch A Pathol Anat Histol. 1974;364(1):111–127. doi: 10.1007/BF01230861. [DOI] [PubMed] [Google Scholar]
- KATCHALSKY A., DANON D., NEVO A. Interactions of basic polyelectrolytes with the red blood cell. II. Agglutination of red blood cells by polymeric bases. Biochim Biophys Acta. 1959 May;33(1):120–138. doi: 10.1016/0006-3002(59)90505-0. [DOI] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Majno G., Shea S. M., Leventhal M. Endothelial contraction induced by histamine-type mediators: an electron microscopic study. J Cell Biol. 1969 Sep;42(3):647–672. doi: 10.1083/jcb.42.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marikovsky Y., Inbar M., Danon D., Sachs L. Distribution of surface charge and concanavalin A-binding sites on normal and malignant transformed cells. Exp Cell Res. 1974 Dec;89(2):359–367. doi: 10.1016/0014-4827(74)90801-5. [DOI] [PubMed] [Google Scholar]
- REYNOLDS M. Measurement of bovine plasma and blood volume during pregnancy and lactation. Am J Physiol. 1953 Oct;175(1):118–122. doi: 10.1152/ajplegacy.1953.175.1.118. [DOI] [PubMed] [Google Scholar]
- Sawyer P. N., Srinivasan S. The role of electrochemical surface properties in thrombosis at vascular interfaces: cumulative experience of studies in animals and man. Bull N Y Acad Med. 1972 Feb;48(2):235–256. [PMC free article] [PubMed] [Google Scholar]
- Skutelsky E., Rudich Z., Danon D. Surface charge properties of the luminal front of blood vessel walls: an electron microscopical analysis. Thromb Res. 1975 Oct;7(4):623–634. doi: 10.1016/0049-3848(75)90108-5. [DOI] [PubMed] [Google Scholar]
- Subjeck J. R., Weiss L. The binding of cationized ferritin at the surfaces of ehrlich ascites tumor cells: the effect of pH and glutaraldehyde fixation. J Cell Physiol. 1975 Jun;85(3):529–536. doi: 10.1002/jcp.1040850304. [DOI] [PubMed] [Google Scholar]
- Yahara I., Edelman G. M. Electron microscopic analysis of the modulation of lymphocyte receptor mobility. Exp Cell Res. 1975 Mar 1;91(1):125–142. doi: 10.1016/0014-4827(75)90150-0. [DOI] [PubMed] [Google Scholar]