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. 1976 Oct 1;71(1):314–322. doi: 10.1083/jcb.71.1.314

Concanavalin a and wheat germ agglutinin receptors on dictyostelium: Their visualization by scanning electron microscopy with microspheres

R Molday, R Jaffe, D McMahon
PMCID: PMC2109733  PMID: 988030

Abstract

The cellular slime mold, Dictyostelium discoideum, is a convenient model for studying cellular interactions during development. Evidence that specific cell surface components are involved in cellular interactions during its development has been obtained by Gerisch and co-workers (1, 2) using immunological techniques. Smart and Hynes (3) have shown that a cell surface protein can be iodinated on cells in aggregation phase, but not in vegetative phase, by the lactoperoxidase procedure. Recently, McMahon et al. (4), and Hoffman and McMahon have demonstrated, by SDS gel electrophoresis, considerable differences in cell surface proteins and glycoproteins of plasma membranes isolated from cells at different stages of development. Plant lectins have also been used to monitor changes in cell surface properties of D. discoideum cells during development. Weeks and co-workers (5, 6) have detected differences in the binding and agglutination of cells by concanavalin A (Con A). Gillette and Filosa (7) have shown that Con A inhibits cell aggregation and prematurely induces cyclic AMP phosphodiesterase. Capping of Con A receptors has also been reported (8). Reitherman et al. (9) have recently reported that agglutination of cells by several plant lectins and the slime mold agglutination, discoidin, changes during development. Such studies indicate that differences in surface properties exist for cells at various stages of development. However, owing to the uncertainties in the factors which contribute to lectin-induced cell agglutination (10), the molecular basis for these observations remain to be determined. In this study, we have used microspheres (11-14) coupled to either Con A or wheat germ agglutinin (WGA) as visual markers to study by scanning electron microscopy the topographical distribution of lectin receptors on D. discoideum cells fixed at different stages of development. We also describe the effect of labeling on the distribution of lectin receptors and on the morphology of the cell surface.

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Selected References

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  1. Beug H., Gerisch G., Kempff S., Riedel V., Cremer G. Specific inhibition of cell contact formation in Dictyostelium by univalent antibodies. Exp Cell Res. 1970 Nov;63(1):147–158. doi: 10.1016/0014-4827(70)90343-5. [DOI] [PubMed] [Google Scholar]
  2. Beug H., Katz F. E., Gerisch G. Dynamics of antigenic membrane sites relating to cell aggregation in Dictyostelium discoideum. J Cell Biol. 1973 Mar;56(3):647–658. doi: 10.1083/jcb.56.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clarke M., Schatten G., Mazia D., Spudich J. A. Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 May;72(5):1758–1762. doi: 10.1073/pnas.72.5.1758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clarke M., Spudich J. A. Biochemical and structural studies of actomyosin-like proteins from non-muscle cells. Isolation and characterization of myosin from amoebae of Dictyostelium discoideum. J Mol Biol. 1974 Jun 25;86(2):209–222. doi: 10.1016/0022-2836(74)90013-8. [DOI] [PubMed] [Google Scholar]
  5. Edelman G. M., Yahara I., Wang J. L. Receptor mobility and receptor-cytoplasmic interactions in lymphocytes. Proc Natl Acad Sci U S A. 1973 May;70(5):1442–1446. doi: 10.1073/pnas.70.5.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gillette M. U., Dengler R. E., Filosa M. F. The localization and fate of concanavalin A in amoebae of the cellular slime mold, Dictyostelium discoideum. J Exp Zool. 1974 Nov;190(2):243–248. doi: 10.1002/jez.1401900213. [DOI] [PubMed] [Google Scholar]
  7. Gillette M. U., Filosa M. F. Effect of concanavalin A on cellular slime mold development: premature appearance of membrane-bound cyclic AMP phosphodiesterase. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1159–1166. doi: 10.1016/0006-291x(73)90586-x. [DOI] [PubMed] [Google Scholar]
  8. Loomis W. F., Jr Sensitivity of Dictyostelium discoideum to nucleic acid analogues. Exp Cell Res. 1971 Feb;64(2):484–486. doi: 10.1016/0014-4827(71)90107-8. [DOI] [PubMed] [Google Scholar]
  9. McMahon D. A cell-contact model for cellular position determination in development. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2396–2400. doi: 10.1073/pnas.70.8.2396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Molday R. S. A scanning electron microscope study of concanavalin A receptors on retinal rod cells labeled with latex microspheres. J Supramol Struct. 1976;4(4):549–557. doi: 10.1002/jss.400040414. [DOI] [PubMed] [Google Scholar]
  11. Molday R. S., Dreyer W. J., Rembaum A., Yen S. P. New immunolatex spheres: visual markers of antigens on lymphocytes for scanning electron microscopy. J Cell Biol. 1975 Jan;64(1):75–88. doi: 10.1083/jcb.64.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Newell P. C., Telser A., Sussman M. Alternative developmental pathways determined by environmental conditions in the cellular slime mold Dictyostelium discoideum. J Bacteriol. 1969 Nov;100(2):763–768. doi: 10.1128/jb.100.2.763-768.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nicolson G. L. The interactions of lectins with animal cell surfaces. Int Rev Cytol. 1974;39:89–190. doi: 10.1016/s0074-7696(08)60939-0. [DOI] [PubMed] [Google Scholar]
  14. Oliver J. M., Zurier R. B., Berlin R. D. Concanavalin a cap formation on polymorphonuclear leukocytes of normal and beige (chediak-higashi) mice. Nature. 1975 Feb 6;253(5491):471–473. doi: 10.1038/253471a0. [DOI] [PubMed] [Google Scholar]
  15. Reitherman R. W., Rosen S. D., Frasier W. A., Barondes S. H. Cell surface species-specific high affinity receptors for discoidin: developmental regulation in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3541–3545. doi: 10.1073/pnas.72.9.3541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rosenblith J. Z., Ukena T. E., Yin H. H., Berlin R. D., Karnovsky M. J. A comparative evaluation of the distribution of concanavalin A-binding sites on the surfaces of normal, virally-transformed, and protease-treated fibroblasts. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1625–1629. doi: 10.1073/pnas.70.6.1625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smart J. E., Hynes R. O. Developmentally regulated cell surface alterations in Dictyostelium discoideum. Nature. 1974 Sep 27;251(5473):319–321. doi: 10.1038/251319a0. [DOI] [PubMed] [Google Scholar]
  18. Weeks C., Weeks G. Cell surface changes during the differentiation of Dictyostelium discoideum. Interaction of cells with Concanavalin A. Exp Cell Res. 1975 May;92(2):372–382. doi: 10.1016/0014-4827(75)90391-2. [DOI] [PubMed] [Google Scholar]
  19. Weeks G. Studies of the cell surface of Dictyostelium discoideum during differentiation. The binding of 125I-concanavalin A to the cell surface. J Biol Chem. 1975 Sep 10;250(17):6706–6710. [PubMed] [Google Scholar]

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