Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1976 Nov 1;71(2):670–674. doi: 10.1083/jcb.71.2.670

A correlation between membrane fluidity and the critical temperature for cell adhesion

PMCID: PMC2109761  PMID: 993265

Abstract

BHK 21 cells can adhere to a protein-coated plastic dish in the presence of Ca2+ at temperatures above 12 degrees C. However, they cannot adhere below 8 degrees C. The ESR spectrum of cells spin-labeled with a stearic acid label indicated that the membrane fluidity changed characteristically at 10 degrees C, 20 degrees C, and 30 degrees C. The critical temperature for cell adhesion coincided well with one of the characteristic temperatures for the membrane fluidity change. In the case of adhesion in the presence of Mg2+, no such correlation was observed.

Full Text

The Full Text of this article is available as a PDF (296.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen J. E., Rasmussen H. Human red blood cells: prostaglandin E2, epinephrine, and isoproterenol alter deformability. Science. 1971 Oct 29;174(4008):512–514. doi: 10.1126/science.174.4008.512. [DOI] [PubMed] [Google Scholar]
  2. Good R. J. Theory of the adhesion of cells and the spontaneous sorting-out of mixed cell aggregates. J Theor Biol. 1972 Dec;37(3):413–434. doi: 10.1016/0022-5193(72)90083-5. [DOI] [PubMed] [Google Scholar]
  3. Grant C. W., McConnell H. M. Glycophorin in lipid bilayers. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4653–4657. doi: 10.1073/pnas.71.12.4653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Horwitz A. F., Hatten M. E., Burger M. M. Membrane fatty acid replacements and their effect on growth and lectin-induced agglutinability. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3115–3119. doi: 10.1073/pnas.71.8.3115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hubbell W. L., McConnell H. M. Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc. 1971 Jan 27;93(2):314–326. doi: 10.1021/ja00731a005. [DOI] [PubMed] [Google Scholar]
  6. Iot T., Ohnish S., Ishinaga M., Kito M. Synthesis of a new phosphatidylserine spin-label and calcium-induced lateral phase separation in phosphatidylserine-phosphatidylcholine membranes. Biochemistry. 1975 Jul 15;14(14):3064–3069. doi: 10.1021/bi00685a004. [DOI] [PubMed] [Google Scholar]
  7. Jost P., Libertini L. J., Hebert V. C., Griffith O. H. Lipid spin labels in lecithin multilayers. A study of motion along fatty acid chains. J Mol Biol. 1971 Jul 14;59(1):77–98. doi: 10.1016/0022-2836(71)90414-1. [DOI] [PubMed] [Google Scholar]
  8. Kleemann W., McConnell H. M. Lateral phase separations in Escherichia coli membranes. Biochim Biophys Acta. 1974 Apr 29;345(2):220–230. doi: 10.1016/0005-2736(74)90260-0. [DOI] [PubMed] [Google Scholar]
  9. Kornberg R. D., McConnell H. M. Lateral diffusion of phospholipids in a vesicle membrane. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2564–2568. doi: 10.1073/pnas.68.10.2564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kury P. G., Ramwell P. W., McConnell H. M. The effect of prostaglandins E1 and E2 on the human erythrocyte as monitored by spin labels. Biochem Biophys Res Commun. 1974 Jan 23;56(2):478–483. doi: 10.1016/0006-291x(74)90867-5. [DOI] [PubMed] [Google Scholar]
  11. Lelievre L. Plasma membranes from fibroblastic cells in culture. Isolation, morphological and enzymatic identification. Biochim Biophys Acta. 1973 Feb 16;291(3):662–670. doi: 10.1016/0005-2736(73)90471-9. [DOI] [PubMed] [Google Scholar]
  12. Lilien J. E. Specific enhancement of cell aggregation in vitro. Dev Biol. 1968 Jun;17(6):657–678. doi: 10.1016/0012-1606(68)90012-2. [DOI] [PubMed] [Google Scholar]
  13. Linden C. D., Wright K. L., McConnell H. M., Fox C. F. Lateral phase separations in membrane lipids and the mechanism of sugar transport in Escherichia coli. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2271–2275. doi: 10.1073/pnas.70.8.2271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MOSCONA A. Rotation-mediated histogenetic aggregation of dissociated cells. A quantifiable approach to cell interactions in vitro. Exp Cell Res. 1961 Jan;22:455–475. doi: 10.1016/0014-4827(61)90122-7. [DOI] [PubMed] [Google Scholar]
  15. Moscona A. A., Moscona M. H. Aggregation of embryonic cells in a serum-free medium and its inhibition at suboptimal temperatures. Exp Cell Res. 1966 Mar;41(3):697–702. doi: 10.1016/s0014-4827(66)80126-x. [DOI] [PubMed] [Google Scholar]
  16. Onishi S., Ito T. Calcium-induced phase separations in phosphatidylserine--phosphatidylcholine membranes. Biochemistry. 1974 Feb 26;13(5):881–887. doi: 10.1021/bi00702a008. [DOI] [PubMed] [Google Scholar]
  17. PUCK T. T., CIECIURA S. J., ROBINSON A. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 1958 Dec 1;108(6):945–956. doi: 10.1084/jem.108.6.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Petit V. A., Edidin M. Lateral phase separation of lipids in plasma membranes: effect of temperature on the mobility of membrane antigens. Science. 1974 Jun 14;184(4142):1183–1185. doi: 10.1126/science.184.4142.1183. [DOI] [PubMed] [Google Scholar]
  19. Rabinovitch M., Destefano M. J. Macrophage spreading in vitro. II. Manganese and other metals as inducers or as co-factors for induced spreading. Exp Cell Res. 1973 Jun;79(2):423–430. doi: 10.1016/0014-4827(73)90462-x. [DOI] [PubMed] [Google Scholar]
  20. Rittenhouse H. G., Williams R. E., Wisnieski B., Fox C. F. Alterations of characteristic temperatures for lectin interactions in LM cells with altered lipid composition. Biochem Biophys Res Commun. 1974 May 7;58(1):222–228. doi: 10.1016/0006-291x(74)90915-2. [DOI] [PubMed] [Google Scholar]
  21. Roth S., McGuire E. J., Roseman S. An assay for intercellular adhesive specificity. J Cell Biol. 1971 Nov;51(21):525–535. doi: 10.1083/jcb.51.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scandella C. J., Devaux P., McConnell H. M. Rapid lateral diffusion of phospholipids in rabbit sarcoplasmic reticulum. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2056–2060. doi: 10.1073/pnas.69.8.2056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shimshick E. J., McConnell H. M. Lateral phase separation in phospholipid membranes. Biochemistry. 1973 Jun 5;12(12):2351–2360. doi: 10.1021/bi00736a026. [DOI] [PubMed] [Google Scholar]
  24. Steinberg M. S., Armstrong P. B., Granger R. E. On the recovery of adhesiveness by trypsin-dissociated cells. J Membr Biol. 1973;13(2):97–128. doi: 10.1007/BF01868223. [DOI] [PubMed] [Google Scholar]
  25. Takeichi M., Okada T. S. Roles of magnesium and calcium ions in cell-to-substrate adhesion. Exp Cell Res. 1972 Sep;74(1):51–60. doi: 10.1016/0014-4827(72)90480-6. [DOI] [PubMed] [Google Scholar]
  26. Weinbaum G., Burger M. M. Two component system for surface guided reassociation of animal cells. Nature. 1973 Aug 24;244(5417):510–512. doi: 10.1038/244510a0. [DOI] [PubMed] [Google Scholar]
  27. Wisnieski B. J., Parkes J. G., Huang Y. O., Fox C. F. Physical and physiological evidence for two phase transitions in cytoplasmic membranes of animal cells. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4381–4385. doi: 10.1073/pnas.71.11.4381. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES