Abstract
RNA synthesis, previously shown to take place during oocyte growth, has been demonstrated throughout the growth-quiescent period preceding ovulation of the mouse oocyte. In the final 7-day preovulatory period, the level of incorporation of (5,6-3H)uridine into ovulated oocytes decreased as the interval between exposure to precursor and ovulation decreased; significant incorporation was detectable within 2 days before ovulation. Analysis of the frequency and density of label in ovarian oocytes at successive stages of meiosis in relation to the interval between adminstration of labeled precursor and collection of oocytes revealed that RNA synthesis continues up to within 2 h before GVBD.
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- Bachvarova R. Incorporation of tritiated adenosine into mouse ovum RNA. Dev Biol. 1974 Sep;40(1):52–58. doi: 10.1016/0012-1606(74)90106-7. [DOI] [PubMed] [Google Scholar]
- Bloom A. M., Mukherjee B. B. RNA synthesis in maturing mouse oocytes. Exp Cell Res. 1972 Oct;74(2):577–582. doi: 10.1016/0014-4827(72)90421-1. [DOI] [PubMed] [Google Scholar]
- Borum K. Oogenesis in the mouse. A study of the origin of the mature ova. Exp Cell Res. 1967 Jan;45(1):39–47. doi: 10.1016/0014-4827(67)90110-3. [DOI] [PubMed] [Google Scholar]
- Brown D. D., Littna E. Synthesis and accumulation of DNA-like RNA during embryogenesis of Xenopus laevis. J Mol Biol. 1966 Sep;20(1):81–94. doi: 10.1016/0022-2836(66)90119-7. [DOI] [PubMed] [Google Scholar]
- Cohen B. B., Deane D. L. Template activity of unfixed metaphase chromosomes. J Cell Sci. 1976 Jan;20(1):215–219. doi: 10.1242/jcs.20.1.215. [DOI] [PubMed] [Google Scholar]
- EDWARDS R. G., GATES A. H. Timing of the stages of the maturation divisions, ovulation, fertilization and the first cleavage of eggs of adult mice treated with gonadotrophins. J Endocrinol. 1959 May;18(3):292–304. doi: 10.1677/joe.0.0180292. [DOI] [PubMed] [Google Scholar]
- Moore G. P., Lintern-Moore S., Peters H., Faber M. RNA synthesis in the mouse oocyte. J Cell Biol. 1974 Feb;60(2):416–422. doi: 10.1083/jcb.60.2.416. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oakberg E. F. Relationship between stage of follicular development and RNA synthesis in the mouse oocyte. Mutat Res. 1968 Jul-Aug;6(1):155–165. doi: 10.1016/0027-5107(68)90111-5. [DOI] [PubMed] [Google Scholar]
- Pedersen T. Follicle kinetics in the ovary of the cyclic mouse. Acta Endocrinol (Copenh) 1970 Jun;64(2):304–323. doi: 10.1530/acta.0.0640304. [DOI] [PubMed] [Google Scholar]
- Rodman T. C., Biedler J. L. Specificity of compaction in meiotic chromosomes of the female Chinese hamster. Chromosoma. 1973 Jun 28;42(3):229–246. doi: 10.1007/BF00284773. [DOI] [PubMed] [Google Scholar]
- Rodman T. C. Chromosomes of the first polar body in mammalian meiosis. Exp Cell Res. 1971 Sep;68(1):205–210. doi: 10.1016/0014-4827(71)90604-5. [DOI] [PubMed] [Google Scholar]
- Schuetz A. W. Role of hormones in oocyte maturation. Biol Reprod. 1974 Mar;10(2):150–178. doi: 10.1095/biolreprod10.2.150. [DOI] [PubMed] [Google Scholar]
- Stern S., Rayyis A., Kennedy J. F. Incoporation of amino acids during maturation in vitro by the mouse oocyte: effect of puromycin on protein synthesis. Biol Reprod. 1972 Dec;7(3):341–346. doi: 10.1093/biolreprod/7.3.341. [DOI] [PubMed] [Google Scholar]