Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1976 Jul 1;70(1):9–19. doi: 10.1083/jcb.70.1.9

Dependence of centriole formation on protein synthesis

PMCID: PMC2109815  PMID: 932104

Abstract

Centriole formation was studied after inhibition of protein synthesis for various portions of the cell cycle. Synchronous populations of mitotic L929 (mouse) cells were plated into petri dishes and the course of procentriole formation was monitored by electron microscope analysis. The frequency with which procentrioles were seen in association with mature centrioles normally increased steadily in the interval from 4 to 12 h after mitosis. The formation of procentrioles was abruptly inhibited by the addition of cycloheximide at any time from mitosis until 12 h postmitosis (S phase). This suggested that the formation of procentrioles was dependent upon protein synthesis immediately before their appearance. Prophase-accociated elongation of procentrioles appeared to occur normally in cells treated with cycloheximide for up to 4 h before prophase, though the mitotic index in treated cultures decreased somewhat. Thus, protein synthesis did not appear to be essential for procentriolar elongation to the mature length.

Full Text

The Full Text of this article is available as a PDF (2.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bacchetti S., Sinclair W. K. The relation of protein synthesis to radiation-induced division delay in Chinese hamster cells. Radiat Res. 1970 Dec;44(3):788–806. [PubMed] [Google Scholar]
  2. Brinkley B. R., Stubblefield E., Hsu T. C. The effects of colcemid inhibition and reversal on the fine structure of the mitotic apparatus of Chinese hamster cells in vitro. J Ultrastruct Res. 1967 Jul;19(1):1–18. doi: 10.1016/s0022-5320(67)80057-1. [DOI] [PubMed] [Google Scholar]
  3. DeFoor P. H., Stubblefield E. Effects of actinomycin D, amethopterin, and 5-fluro-2'-deoxyuridine on procentriole formation in Chinese hamster fibroblasts in culture. Exp Cell Res. 1974 Mar 30;85(1):136–142. doi: 10.1016/0014-4827(74)90223-7. [DOI] [PubMed] [Google Scholar]
  4. Dirksen E. R., Staprans I. Tubulin synthesis during ciliogenesis in the mouse oviduct. Dev Biol. 1975 Sep;46(1):1–13. doi: 10.1016/0012-1606(75)90082-2. [DOI] [PubMed] [Google Scholar]
  5. Doida Y., Okada S. Radiation-induced mitotic delay in cultured mammalian cells (L5178Y). Radiat Res. 1969 Jun;38(3):513–529. [PubMed] [Google Scholar]
  6. Forrest G. L., Klevecz R. R. Synthesis and degradation of microtubule protein in synchronized Chinese hamster cells. J Biol Chem. 1972 May 25;247(10):3147–3152. [PubMed] [Google Scholar]
  7. GALL J. G. Centriole replication. A study of spermatogenesis in the snail Viviparus. J Biophys Biochem Cytol. 1961 Jun;10:163–193. doi: 10.1083/jcb.10.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hodge L. D., Robbins E., Scharff M. D. Persistence of messenger RNA through mitosis in HeLa cells. J Cell Biol. 1969 Feb;40(2):497–507. doi: 10.1083/jcb.40.2.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kowit J. D., Fulton C. Programmed synthesis of tubulin for the flagella that develop during cell differentiation in Naegleria gruberi. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2877–2881. doi: 10.1073/pnas.71.7.2877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Randall J., Disbrey C. Evidence for the presence of DNA at basal body sites in Tetrahymena pyriformis. Proc R Soc Lond B Biol Sci. 1965 Jul 27;162(989):473–491. doi: 10.1098/rspb.1965.0051. [DOI] [PubMed] [Google Scholar]
  11. Rannestad J. The regeneration of cilia in partially deciliated Tetrahymena. J Cell Biol. 1974 Dec;63(3):1009–1017. doi: 10.1083/jcb.63.3.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rattner J. B., Phillips S. G. Independence of centriole formation and DNA synthesis. J Cell Biol. 1973 May;57(2):359–372. doi: 10.1083/jcb.57.2.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robbins E., Jentzsch G., Micali A. The centriole cycle in synchronized HeLa cells. J Cell Biol. 1968 Feb;36(2):329–339. doi: 10.1083/jcb.36.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roos U. P. Light and electron microscopy of rat kangaroo cells in mitosis. I. Formation and breakdown of the mitotic apparatus. Chromosoma. 1973;40(1):43–82. doi: 10.1007/BF00319836. [DOI] [PubMed] [Google Scholar]
  15. Rosenbaum J. L., Child F. M. Flagellar regeneration in protozoan flagellates. J Cell Biol. 1967 Jul;34(1):345–364. doi: 10.1083/jcb.34.1.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rosenbaum J. L., Moulder J. E., Ringo D. L. Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins. J Cell Biol. 1969 May;41(2):600–619. doi: 10.1083/jcb.41.2.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SOROKIN S. Centrioles and the formation of rudimentary cilia by fibroblasts and smooth muscle cells. J Cell Biol. 1962 Nov;15:363–377. doi: 10.1083/jcb.15.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SOTELO J. R., TRUJILLO-CENOZ O. Electron microscope study on the development of ciliary components of the neural epithelium of the chick embryo. Z Zellforsch Mikrosk Anat. 1958;49(1):1–12. doi: 10.1007/BF00335059. [DOI] [PubMed] [Google Scholar]
  19. Schneiderman M. H., Dewey W. C., Leeper D. B., Nagasawa H. Use of the mitotic selection procedure for cell cycle analysis. Comparison between the X-ray and cycloheximide G2 markers. Exp Cell Res. 1972 Oct;74(2):430–438. doi: 10.1016/0014-4827(72)90398-9. [DOI] [PubMed] [Google Scholar]
  20. Sisken J. E., Wilkes E. The time of synthesis and the conservation of mitosis-related proteins in cultured human amnion cells. J Cell Biol. 1967 Jul;34(1):97–110. doi: 10.1083/jcb.34.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith-Sonneborn J., Plaut W. Evidence for the presence of DNA in the pellicle of Paramecium. J Cell Sci. 1967 Jun;2(2):225–234. doi: 10.1242/jcs.2.2.225. [DOI] [PubMed] [Google Scholar]
  22. Staprans I., Dirksen E. R. Microtubule protein during ciliogenesis in the mouse oviduct. J Cell Biol. 1974 Jul;62(1):164–174. doi: 10.1083/jcb.62.1.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TERASIMA T., TOLMACH L. J. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res. 1963 Apr;30:344–362. doi: 10.1016/0014-4827(63)90306-9. [DOI] [PubMed] [Google Scholar]
  24. Tobey R. A., Anderson E. C., Petersen D. F. RNA stability and protein synthesis in relation to the division of mammalian cells. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1520–1527. doi: 10.1073/pnas.56.5.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tobey R. A., Petersen D. F., Anderson E. C., Puck T. T. Life cycle analysis of mammalian cells. 3. The inhibition of division in Chinese hamster cells by puromycin and actinomycin. Biophys J. 1966 Sep;6(5):567–581. doi: 10.1016/s0006-3495(66)86678-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Walters R. A., Petersen D. F. Radiosensitivity of mammalian cells. II. Radiation effects on macromolecular synthesis. Biophys J. 1968 Dec;8(12):1487–1504. doi: 10.1016/S0006-3495(68)86568-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Williams N. E., Nelsen E. M. Regulation of microtubules in Tetrahymena. II. Relation between turnover of microtubule proteins and microtubule dissociation and assembly during oral replacement. J Cell Biol. 1973 Feb;56(2):458–465. doi: 10.1083/jcb.56.2.458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Younger K. B., Banerjee S., Kelleher J. K., Winston M., Margulis L. Evidence that the synchronized production of new basal bodies is not associated with DNA synthesis in Stentor coeruleus. J Cell Sci. 1972 Sep;11(2):621–637. doi: 10.1242/jcs.11.2.621. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES