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. 1976 Sep 1;70(3):494–505. doi: 10.1083/jcb.70.3.494

Lipid vesicle-cell interactions. I. Hemagglutination and hemolysis

PMCID: PMC2109851  PMID: 956268

Abstract

The interaction of lipid vesicles (liposomes) of several different compositions with erythrocytes has been investigated. Lecithin liposomes, rendered positively charged with stearylamine, exhibit potent hemagglutination activity in media containing low concentrations of electrolytes. The hemagglutination titer is found to be a linear function of the zeta potential of the lipid vesicles. Hemagglutination is reduced when the surface potential of the cells is made more positive by pH adjustment or enzyme treatment. Similarly, hemagglutination is reduced by increasing concentrations of electrolytes. Hemagglutination is examined theoretically and is shown to be consistent with vesicle-cell interactions that are due to only electrostatic forces. Vesicles containing lysolecithin in addition to lecithin and stearylamine cause lysis of erythrocytes, provided the lipids of the vesicles are above the crystal-liquid crystal phase transition temperature. In addition, hemolysis requires close juxtaposition of the vesicle to the cell membrane; vesicles precoated with antibodies exhibit severely diminished hemolytic activities, only a small fraction of which can be attributed to a reduction in hemagglutination titer. Evidence is presented indicating that a single vesicle is sufficient to lyse one cell. With regard to hemagglutination and hemolysis, lipid vesicles of simple composition mimic paramyxoviruses such as Sendai virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
  2. BANGHAM A. D., HORNE R. W. NEGATIVE STAINING OF PHOSPHOLIPIDS AND THEIR STRUCTURAL MODIFICATION BY SURFACE-ACTIVE AGENTS AS OBSERVED IN THE ELECTRON MICROSCOPE. J Mol Biol. 1964 May;8:660–668. doi: 10.1016/s0022-2836(64)80115-7. [DOI] [PubMed] [Google Scholar]
  3. Barton P. G. The influence of surface charge density of phosphatides on the binding of some cations. J Biol Chem. 1968 Jul 25;243(14):3884–3890. [PubMed] [Google Scholar]
  4. Batzri S., Korn E. D. Interaction of phospholipid vesicles with cells. Endocytosis and fusion as alternate mechanisms for the uptake of lipid-soluble and water-soluble molecules. J Cell Biol. 1975 Sep;66(3):621–634. doi: 10.1083/jcb.66.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grant C. W., McConnell H. M. Fusion of phospholipid vesicles with viable Acholeplasma laidlawii. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1238–1240. doi: 10.1073/pnas.70.4.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gregoriadis G., Neerunjun E. D. Homing of liposomes to target cells. Biochem Biophys Res Commun. 1975 Jul 22;65(2):537–544. doi: 10.1016/s0006-291x(75)80180-x. [DOI] [PubMed] [Google Scholar]
  7. Haydon D. A., Taylor J. The stability and properties of bimolecular lipid leaflets in aqueous solutions. J Theor Biol. 1963 May;4(3):281–296. doi: 10.1016/0022-5193(63)90007-9. [DOI] [PubMed] [Google Scholar]
  8. Howe C., Morgan C. Interactions between Sendai virus and human erythrocytes. J Virol. 1969 Jan;3(1):70–81. doi: 10.1128/jvi.3.1.70-81.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huang L., Pagano R. E. Interaction of phospholipid vesicles with cultured mammalial cells. I. Characteristics of uptake. J Cell Biol. 1975 Oct;67(1):38–48. doi: 10.1083/jcb.67.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inbar M., Shinitzky M. Increase of cholesterol level in the surface membrane of lymphoma cells and its inhibitory effect on ascites tumor development. Proc Natl Acad Sci U S A. 1974 May;71(5):2128–2130. doi: 10.1073/pnas.71.5.2128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inoue K., Kitagawa T. Effect of exogenous lysolecithin on liposomal membranes. Its relation to membrane fluidity. Biochim Biophys Acta. 1974 Sep 23;363(3):361–372. doi: 10.1016/0005-2736(74)90075-3. [DOI] [PubMed] [Google Scholar]
  12. Johnson M. E., Simon S., Kauffman J. W., MacDonald R. C. A synthetic lecithin containing branched-chain fatty acids: physical properties and membrane studies. Biochim Biophys Acta. 1973 Feb 16;291(3):587–591. doi: 10.1016/0005-2736(73)90463-x. [DOI] [PubMed] [Google Scholar]
  13. Korn E. D., Bowers B., Batzri S., Simmons S. R., Victoria E. J. Endycytosis and exocytosis: role of microfilaments and involvement of phospholipids in membrane fusion. J Supramol Struct. 1974;2(5-6):517–528. doi: 10.1002/jss.400020502. [DOI] [PubMed] [Google Scholar]
  14. Magee W. E., Goff C. W., Schoknecht J., Smith M. D., Cherian K. The interaction of cationic liposomes containing entrapped horseradish peroxidase with cells in culture. J Cell Biol. 1974 Nov;63(2 Pt 1):492–504. doi: 10.1083/jcb.63.2.492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mandersloot J. G., Reman F. C., Van Deenen L. L., De Gier J. Barrier properties of lecithin/lysolecithin mixtures. Biochim Biophys Acta. 1975 Feb 28;382(1):22–26. doi: 10.1016/0005-2736(75)90368-5. [DOI] [PubMed] [Google Scholar]
  16. Martin F. J., MacDonald R. C. Lipid vesicle-cell interactions. II. Induction of cell fusion. J Cell Biol. 1976 Sep;70(3):506–514. doi: 10.1083/jcb.70.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Martin F. J., MacDonald R. C. Lipid vesicle-cell interactions. III. Introduction of a new antigenic determinant into erythrocyte membranes. J Cell Biol. 1976 Sep;70(3):515–526. doi: 10.1083/jcb.70.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Martin F. J., MacDonald R. C. Phospholipid exchange between bilayer membrane vesicles. Biochemistry. 1976 Jan 27;15(2):321–327. doi: 10.1021/bi00647a013. [DOI] [PubMed] [Google Scholar]
  19. Martin F., MacDonald R. Liposomes can mimic virus membranes. Nature. 1974 Nov 8;252(5479):161–163. doi: 10.1038/252161a0. [DOI] [PubMed] [Google Scholar]
  20. Neurath A. R., Vernon S. K., Hartzell R. W., Rubin B. A. Haemolysis by Sendai virus: lack of requirement for neuraminidase. J Gen Virol. 1972 Aug;16(2):245–249. doi: 10.1099/0022-1317-16-2-245. [DOI] [PubMed] [Google Scholar]
  21. Pagano R. E., Huang L. Interaction of phospholipid vesicles with cultured mammalian cells. II. Studies of mechanism. J Cell Biol. 1975 Oct;67(1):49–60. doi: 10.1083/jcb.67.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pagano R. E., Huang L., Wey C. Interaction of phospholipid vesicles with cultured mammalian cells. Nature. 1974 Nov 8;252(5479):166–167. doi: 10.1038/252166a0. [DOI] [PubMed] [Google Scholar]
  23. Papahadjopoulos D., Mayhew E., Poste G., Smith S., Vail W. J. Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour. Nature. 1974 Nov 8;252(5479):163–166. doi: 10.1038/252163a0. [DOI] [PubMed] [Google Scholar]
  24. Papahadjopoulos D., Poste G., Mayhew E. Cellular uptake of cyclic AMP captured within phospholipid vesicles and effect on cell-growth behaviour. Biochim Biophys Acta. 1974 Sep 23;363(3):404–418. doi: 10.1016/0005-2736(74)90079-0. [DOI] [PubMed] [Google Scholar]
  25. Papahadjopoulos D., Poste G., Schaeffer B. E. Fusion of mammalian cells by unilamellar lipid vesicles: inflluence of lipid surface charge, fluidity and cholesterol. Biochim Biophys Acta. 1973 Sep 27;323(1):23–42. doi: 10.1016/0005-2736(73)90429-x. [DOI] [PubMed] [Google Scholar]
  26. Parsegian V. A., Gingell D. On the electrostatic interaction across a salt solution between two bodies bearing unequal charges. Biophys J. 1972 Sep;12(9):1192–1204. doi: 10.1016/S0006-3495(72)86155-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tenforde T. Microelectrophoretic studies on the surface chemistry of erythrocytes. Adv Biol Med Phys. 1970;13:43–105. doi: 10.1016/b978-0-12-005213-4.50006-5. [DOI] [PubMed] [Google Scholar]
  28. Weissmann G., Bloomgarden D., Kaplan R., Cohen C., Hoffstein S., Collins T., Gotlieb A., Nagle D. A general method for the introduction of enzymes, by means of immunoglobulin-coated liposomes, into lysosomes of deficient cells. Proc Natl Acad Sci U S A. 1975 Jan;72(1):88–92. doi: 10.1073/pnas.72.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wirtz K. W. Transfer of phospholipids between membranes. Biochim Biophys Acta. 1974 Sep 16;344(2):95–117. doi: 10.1016/0304-4157(74)90001-x. [DOI] [PubMed] [Google Scholar]

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