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. 1976 Sep 1;70(3):506–514. doi: 10.1083/jcb.70.3.506

Lipid vesicle-cell interactions. II. Induction of cell fusion

PMCID: PMC2109854  PMID: 986397

Abstract

The ability of lipid vesicles of simple composition (lecithin, lysolecithin, and stearylamine) to induce cells of various types to fuse has been investigated. One in every three or four cells in monolayer cultures can be induced to fuse with a vesicle dose of about 100 per cell. At such dosages and for exposures of 15 min to 1 h, vesicles have essentially no effect on cell viability. Under anaerobic conditions, these cells lyse rather than fuse. Avian erythrocytes are readily fused with lipid vesicles in the presence of dextran. Fusion indices increase linearly with the zeta potential of the vesicles (increasing stearylamine content), indicating that contact between vesicle and cell membrane is required. Fusion indices increase sublinearly with increasing lysolecithin content. Divalent cations increase fusion indices at high vesicle doses. The data presented are consistent with the hypothesis that cell fusion occurs via simultaneous fusion of a vesicle with two adhering cell membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahkong Q. F., Cramp F. C., Fisher D., Howell J. I., Tampion W., Verrinder M., Lucy J. A. Chemically-induced and thermally-induced cell fusion: lipid-lipid interactions. Nat New Biol. 1973 Apr 18;242(120):215–217. doi: 10.1038/newbio242215a0. [DOI] [PubMed] [Google Scholar]
  2. Ahkong Q. F., Fisher D., Tampion W., Lucy J. A. The fusion of erythrocytes by fatty acids, esters, retinol and alpha-tocopherol. Biochem J. 1973 Sep;136(1):147–155. doi: 10.1042/bj1360147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Apostolov K., Almeida J. D. Interaction of Sendai (HVJ) virus with human erythrocytes: a morphological study of haemolysis cell fusion. J Gen Virol. 1972 Jun;15(3):227–234. doi: 10.1099/0022-1317-15-3-227. [DOI] [PubMed] [Google Scholar]
  4. Croce C. M., Sawicki W., Kritchevsky D., Koprowski H. Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin. Exp Cell Res. 1971 Aug;67(2):427–435. doi: 10.1016/0014-4827(71)90428-9. [DOI] [PubMed] [Google Scholar]
  5. Hosaka Y., Semba T., Fukai K. Artificial assembly of envelope particles of HVJ (Sendai virus). Fusion activity of envelope particles. J Gen Virol. 1974 Dec;25(3):391–404. doi: 10.1099/0022-1317-25-3-391. [DOI] [PubMed] [Google Scholar]
  6. KOHN A. POLYKARYOCYTOSIS INDUCED BY NEWCASTLE DISEASE VIRUS IN MONOLAYERS OF ANIMAL CELLS. Virology. 1965 Jun;26:228–245. doi: 10.1016/0042-6822(65)90050-4. [DOI] [PubMed] [Google Scholar]
  7. Keay L., Weiss S. A., Cirulis N., Wildi B. S. Lysolecithin-induced fusion of fibroblasts. In Vitro. 1972 Jul-Aug;8(1):19–25. doi: 10.1007/BF02617939. [DOI] [PubMed] [Google Scholar]
  8. Lucy J. A. The fusion of biological membranes. Nature. 1970 Aug 22;227(5260):815–817. doi: 10.1038/227815a0. [DOI] [PubMed] [Google Scholar]
  9. Martin F. J., MacDonald R. C. Lipid vesicle-cell interactions. I. Hemagglutination and hemolysis. J Cell Biol. 1976 Sep;70(3):494–505. doi: 10.1083/jcb.70.3.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martin F. J., MacDonald R. C. Lipid vesicle-cell interactions. III. Introduction of a new antigenic determinant into erythrocyte membranes. J Cell Biol. 1976 Sep;70(3):515–526. doi: 10.1083/jcb.70.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin F. J., MacDonald R. C. Phospholipid exchange between bilayer membrane vesicles. Biochemistry. 1976 Jan 27;15(2):321–327. doi: 10.1021/bi00647a013. [DOI] [PubMed] [Google Scholar]
  12. Martin F., MacDonald R. Liposomes can mimic virus membranes. Nature. 1974 Nov 8;252(5479):161–163. doi: 10.1038/252161a0. [DOI] [PubMed] [Google Scholar]
  13. Okada Y., Murayama F. Requirement of calcium ions for the cell fusion reaction of animal cells by HVJ. Exp Cell Res. 1966 Nov-Dec;44(2):527–551. doi: 10.1016/0014-4827(66)90458-7. [DOI] [PubMed] [Google Scholar]
  14. Okada Y., Murayama F., Yamada K. Requirement of energy for the cell fusion reaction of Ehrlich ascites tumor cells by HVJ. Virology. 1966 Jan;28(1):115–130. doi: 10.1016/0042-6822(66)90312-6. [DOI] [PubMed] [Google Scholar]
  15. Papahadjopoulos D., Poste G., Schaeffer B. E. Fusion of mammalian cells by unilamellar lipid vesicles: inflluence of lipid surface charge, fluidity and cholesterol. Biochim Biophys Acta. 1973 Sep 27;323(1):23–42. doi: 10.1016/0005-2736(73)90429-x. [DOI] [PubMed] [Google Scholar]
  16. Poole A. R., Howell J. I., Lucy J. A. Lysolecithin and cell fusion. Nature. 1970 Aug 22;227(5260):810–814. doi: 10.1038/227810a0. [DOI] [PubMed] [Google Scholar]
  17. Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]

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