Abstract
Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]alpha-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated alpha-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface A Ch receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2 h.
Full Text
The Full Text of this article is available as a PDF (1.4 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Atkinson P. H., Moyer S. A., Summers D. F. Assembly of vesicular stomatitis virus glycoprotein and matrix protein into HeLa cell plasma membranes. J Mol Biol. 1976 Apr 15;102(3):613–631. doi: 10.1016/0022-2836(76)90338-7. [DOI] [PubMed] [Google Scholar]
- Atkinson P. H. Synthesis and assembly of HeLa cell plasma membrane glycoproteins and proteins. J Biol Chem. 1975 Mar 25;250(6):2123–2134. [PubMed] [Google Scholar]
- CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daniels M. P., Vogel Z. Immunoperoxidase staining of alpha-bungarotoxin binding sites in muscle endplates shows distribution of acetylcholine receptors. Nature. 1975 Mar 27;254(5498):339–341. doi: 10.1038/254339a0. [DOI] [PubMed] [Google Scholar]
- Devreotes P. N., Fambrough D. M. Acetylcholine receptor turnover in membranes of developing muscle fibers. J Cell Biol. 1975 May;65(2):335–358. doi: 10.1083/jcb.65.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Devreotes P. N., Fambrough D. M. Turnover of acetylcholine receptors in skeletal muscle. Cold Spring Harb Symp Quant Biol. 1976;40:237–251. doi: 10.1101/sqb.1976.040.01.025. [DOI] [PubMed] [Google Scholar]
- Devreotes P. N., Gardner J. M., Fambrough D. M. Kinetics of biosynthesis of acetylcholine receptor and subsequent incorporation into plasma membrane of cultured chick skeletal muscle. Cell. 1977 Mar;10(3):365–373. doi: 10.1016/0092-8674(77)90023-x. [DOI] [PubMed] [Google Scholar]
- Hartzell H. C., Fambrough D. M. Acetycholine receptor production and incorporation into membranes of developing muscle fibers. Dev Biol. 1973 Jan;30(1):153–165. doi: 10.1016/0012-1606(73)90054-7. [DOI] [PubMed] [Google Scholar]
- Palade G. Intracellular aspects of the process of protein synthesis. Science. 1975 Aug 1;189(4200):347–358. doi: 10.1126/science.1096303. [DOI] [PubMed] [Google Scholar]
- Patrick J., McMillan J., Wolfson H., O'Brien J. C. Acetylcholine receptor metabolism in a nonfusing muscle cell line. J Biol Chem. 1977 Mar 25;252(6):2143–2153. [PubMed] [Google Scholar]
- Salpeter M. M., Szabo M. Sensitivity in electron microscope autoradiography. I. The effect of radiation dose. J Histochem Cytochem. 1972 Jun;20(6):425–434. doi: 10.1177/20.6.425. [DOI] [PubMed] [Google Scholar]
- Vitetta E. S., Uhr J. W. Immunoglobulins and alloantigens on the surface of lymphoid cells. Biochim Biophys Acta. 1975 Jun 30;415(2):253–271. doi: 10.1016/0304-4157(75)90004-0. [DOI] [PubMed] [Google Scholar]