Abstract
Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules containing 14 protofilaments increased with consecutive repetitions of cold- dissociation followed by reassembly in vitro.
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Selected References
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- Burton P. R., Hinkley R. E., Pierson G. B. Tannic acid-stained microtubules with 12, 13, and 15 protofilaments. J Cell Biol. 1975 Apr;65(1):227–233. doi: 10.1083/jcb.65.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dentler W. L., Granett S., Rosenbaum J. L. Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules. J Cell Biol. 1975 Apr;65(1):237–241. doi: 10.1083/jcb.65.1.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gaskin F., Cantor C. R., Shelanski M. L. Biochemical studies on the in vitro assembly and disassembly of microtubules. Ann N Y Acad Sci. 1975 Jun 30;253:133–146. doi: 10.1111/j.1749-6632.1975.tb19197.x. [DOI] [PubMed] [Google Scholar]
- Green L. H., Brandis J. W., Turner F. R., Raff R. A. Cytoplasmic microtubule proteins of the embryo of Drosophila melanogaster. Biochemistry. 1975 Oct 7;14(20):4487–4491. doi: 10.1021/bi00691a023. [DOI] [PubMed] [Google Scholar]
- Himes R. H., Burton P. R., Gaito J. M. Dimethyl sulfoxide-induced self-assembly of tubulin lacking associated proteins. J Biol Chem. 1977 Sep 10;252(17):6222–6228. [PubMed] [Google Scholar]
- Lee Y. C., Samson F. E., Jr, Houston L. L., Himes R. H. The in vitro polymerization of tubulin from beef brain. J Neurobiol. 1974;5(4):317–330. doi: 10.1002/neu.480050404. [DOI] [PubMed] [Google Scholar]
- Murphy D. B., Borisy G. G. Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2696–2700. doi: 10.1073/pnas.72.7.2696. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shelanski M. L., Gaskin F., Cantor C. R. Microtubule assembly in the absence of added nucleotides. Proc Natl Acad Sci U S A. 1973 Mar;70(3):765–768. doi: 10.1073/pnas.70.3.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tilney L. G., Bryan J., Bush D. J., Fujiwara K., Mooseker M. S., Murphy D. B., Snyder D. H. Microtubules: evidence for 13 protofilaments. J Cell Biol. 1973 Nov;59(2 Pt 1):267–275. doi: 10.1083/jcb.59.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]