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. 1978 Feb 1;76(2):341–359. doi: 10.1083/jcb.76.2.341

Permeability of muscle capillaries to microperoxidase

S L Wissig 1, M C Williams 1
PMCID: PMC2109976  PMID: 10605442

Abstract

In this study we attempted to identify a morphologic counterpart of the small pore of muscle capillaries. The existence of such a pore has been postulated by physiologists to explain the permeability of muscle capillaries to small macromolecules. We injected mice intravenously with microperoxidase (MP) and fixed specimens of diaphragm at intervals of 0-250 s after the injection to localize the tracer by electron microscopy. The small size of MP (1,900 mol wt and 20 A molecular diameter [MD]) ensures its ready passage through the small pore since the latter is thought to be either a cylindrical channel 90 A in diameter or a slit 55 A wide. MP appears in the pericapillary interstitium within 30 s of initiation of its intravenous injection. The patterns of localization of MP observed within clefts between adjacent capillary endothelial cells indicate that some endothelial junctions are permeable to this tracer. Although small vesicles transfer MP across the endothelium, we do not believe that the vesicles transfer substantial amounts of MP into the pericapillary interstitium. We did not obtain evidence that MP crosses the endothelium of capillaries through channels formed either by a single vesicle or by a series of linked vesicles opening simultaneously at both surfaces of the endothelial cell. From our observations we conclude that some endothelial junctions of capillaries are permeable to MP, and that these permeable junctions are a plausible morphologic counterpart of the small pore.

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Selected References

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  1. Bruns R. R., Palade G. E. Studies on blood capillaries. I. General organization of blood capillaries in muscle. J Cell Biol. 1968 May;37(2):244–276. doi: 10.1083/jcb.37.2.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bruns R. R., Palade G. E. Studies on blood capillaries. II. Transport of ferritin molecules across the wall of muscle capillaries. J Cell Biol. 1968 May;37(2):277–299. doi: 10.1083/jcb.37.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Claude P., Goodenough D. A. Fracture faces of zonulae occludentes from "tight" and "leaky" epithelia. J Cell Biol. 1973 Aug;58(2):390–400. doi: 10.1083/jcb.58.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cotran K. S., Karnovsky M. J. Vascular leakage induced by horseradish peroxidase in the rat. Proc Soc Exp Biol Med. 1967 Nov;126(2):557–561. doi: 10.3181/00379727-126-32504. [DOI] [PubMed] [Google Scholar]
  5. Dickerson R. E., Takano T., Eisenberg D., Kallai O. B., Samson L., Cooper A., Margoliash E. Ferricytochrome c. I. General features of the horse and bonito proteins at 2.8 A resolution. J Biol Chem. 1971 Mar 10;246(5):1511–1535. [PubMed] [Google Scholar]
  6. Feder N. A heme-peptide as an ultrastructural tracer. J Histochem Cytochem. 1970 Dec;18(12):911–913. doi: 10.1177/18.12.911. [DOI] [PubMed] [Google Scholar]
  7. Feder N. Microperoxidase. An ultrastructural tracer of low molecular weight. J Cell Biol. 1971 Oct;51(1):339–343. doi: 10.1083/jcb.51.1.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GROTTE G. Passage of dextran molecules across the blood-lymph barrier. Acta Chir Scand Suppl. 1956;211:1–84. [PubMed] [Google Scholar]
  9. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  10. Karnovsky M. J. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol. 1967 Oct;35(1):213–236. doi: 10.1083/jcb.35.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LEVIN O. Electron microscopic observations on cytochrome c. Arch Biochem Biophys. 1962 Sep;Suppl 1:301–308. [PubMed] [Google Scholar]
  12. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Luft J. H. Fine structures of capillary and endocapillary layer as revealed by ruthenium red. Fed Proc. 1966 Nov-Dec;25(6):1773–1783. [PubMed] [Google Scholar]
  14. Luft J. H. Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat Rec. 1971 Nov;171(3):347–368. doi: 10.1002/ar.1091710302. [DOI] [PubMed] [Google Scholar]
  15. PALADE G. E. Blood capillaries of the heart and other organs. Circulation. 1961 Aug;24:368–388. doi: 10.1161/01.cir.24.2.368. [DOI] [PubMed] [Google Scholar]
  16. PAPPENHEIMER J. R. Passage of molecules through capillary wals. Physiol Rev. 1953 Jul;33(3):387–423. doi: 10.1152/physrev.1953.33.3.387. [DOI] [PubMed] [Google Scholar]
  17. Pricam C., Humbert F., Perrelet A., Orci L. A freeze-etch study of the tight junctions of the rat kidney tubules. Lab Invest. 1974 Mar;30(3):286–291. [PubMed] [Google Scholar]
  18. Schildt B. E. Mixing time and blood in normal and traumatized mice. Acta Chir Scand. 1971;137(2):107–111. [PubMed] [Google Scholar]
  19. TRUMP B. F., SMUCKLER E. A., BENDITT E. P. A method for staining epoxy sections for light microscopy. J Ultrastruct Res. 1961 Aug;5:343–348. doi: 10.1016/s0022-5320(61)80011-7. [DOI] [PubMed] [Google Scholar]
  20. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vassar P. S., Hards J. M., Brooks D. E., Hagenberger B., Seaman G. V. Physicochemical effects of aldehydes on the human erythrocyte. J Cell Biol. 1972 Jun;53(3):809–818. doi: 10.1083/jcb.53.3.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vegge T., Winther F. O., Olsen B. R. Horseradish peroxidase in plasma studied by gel filtration. Histochemie. 1971;28(1):16–22. doi: 10.1007/BF00305628. [DOI] [PubMed] [Google Scholar]
  23. Westergaard E., Brightman M. W. Transport of proteins across normal cerebral arterioles. J Comp Neurol. 1973 Nov 1;152(1):17–44. doi: 10.1002/cne.901520103. [DOI] [PubMed] [Google Scholar]

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