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. 1978 Mar 1;76(3):692–704. doi: 10.1083/jcb.76.3.692

Rat liver nuclear skeleton and small molecular weight RNA species

PMCID: PMC2110005  PMID: 564914

Abstract

Small molecular weight RNA species (smwRNAs) were studied in rat liver nuclei with and without chromatin as well as with and without nuclear envelope and nucleoplasm. From all the species identified, only two, N5 and 5Sb, were related to ribosomes. The others were localized exclusively in the nuclear skeleton or the spongelike network that was described in the preceding communication. This network or protein matrix contains a less abundant but exclusive set of molecules designated 5Sa, N1, and 4.5S, as well as other more abundant molecules which also exist in rat liver endoplasmic reticulum but not in polysomes or postribosomal RNP complexes. The smwRNAs behave like HnRNA; they remain located in the nuclear skeleton when nuclei are deprived of nucleoplasm and chromatin. With the information presently available, it is not possible to know whetherer both species are in the same or different RNP complexes and whether some of the smwRNAs contribute to the architecture of the nuclear skeleton. Distinct from any other nuclear RNA species, smwRNAs have two unique properties: facility of extraction, and resistance to nuclear ribonuclease digestion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson R. P., Blobel G. On the attachment of the nuclear pore complex. J Cell Biol. 1974 Sep;62(3):746–754. doi: 10.1083/jcb.62.3.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Artman M., Roth J. S. Chromosomal RNA: an artifact of preparation? J Mol Biol. 1971 Sep 14;60(2):291–301. doi: 10.1016/0022-2836(71)90295-6. [DOI] [PubMed] [Google Scholar]
  3. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eliceiri G. L. Short-lived, small RNAs in the cytoplasm of HeLa cells. Cell. 1974 Sep;3(1):11–14. doi: 10.1016/0092-8674(74)90031-2. [DOI] [PubMed] [Google Scholar]
  5. FLECK A., MUNRO H. N. The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim Biophys Acta. 1962 May 14;55:571–583. doi: 10.1016/0006-3002(62)90836-3. [DOI] [PubMed] [Google Scholar]
  6. Faiferman I., Pogo A. O. Isolation of a nuclear ribonucleoprotein network that contains heterogeneous RNA and is bound to the nuclear envelope. Biochemistry. 1975 Aug 26;14(17):3808–3816. doi: 10.1021/bi00688a013. [DOI] [PubMed] [Google Scholar]
  7. Goldstein L., Ko C. Electrophoretic characterization of shuttling and nonshuttling small nuclear RNAs. Cell. 1974 Aug;2(4):259–269. doi: 10.1016/0092-8674(74)90020-8. [DOI] [PubMed] [Google Scholar]
  8. HYMER W. C., KUFF E. L. ISOLATION OF NUCLEI FROM MAMMALIAN TISSUES THROUGH THE USE OF TRITON X-100. J Histochem Cytochem. 1964 May;12:359–363. doi: 10.1177/12.5.359. [DOI] [PubMed] [Google Scholar]
  9. Holmes D. S., Bonner J. Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid. Biochemistry. 1973 Jun 5;12(12):2330–2338. doi: 10.1021/bi00736a023. [DOI] [PubMed] [Google Scholar]
  10. Lee S. Y., Krsmanovic V., Brawerman G. Attachment of ribosomes to membranes during polysome formation in mouse sarcoma 180 cells. J Cell Biol. 1971 Jun;49(3):683–691. doi: 10.1083/jcb.49.3.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lenk R., Ransom L., Kaufmann Y., Penman S. A cytoskeletal structure with associated polyribosomes obtained from HeLa cells. Cell. 1977 Jan;10(1):67–78. doi: 10.1016/0092-8674(77)90141-6. [DOI] [PubMed] [Google Scholar]
  12. Marzluff W. F., Jr, White E. L., Benjamin R., Huang R. C. Low molecular weight RNA species from chromatin. Biochemistry. 1975 Aug 12;14(16):3715–3724. doi: 10.1021/bi00687a031. [DOI] [PubMed] [Google Scholar]
  13. Miller T. E., Huang C. Y., Pogo A. O. Rat liver nuclear skeleton and ribonucleoprotein complexes containing HnRNA. J Cell Biol. 1978 Mar;76(3):675–691. doi: 10.1083/jcb.76.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Parish J. H., Kirby K. S. Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim Biophys Acta. 1966 Dec 21;129(3):554–562. doi: 10.1016/0005-2787(66)90070-0. [DOI] [PubMed] [Google Scholar]
  15. Perry R. P., La Torre J., Kelley D. E., Greenberg J. R. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim Biophys Acta. 1972 Mar 14;262(2):220–226. doi: 10.1016/0005-2787(72)90236-5. [DOI] [PubMed] [Google Scholar]
  16. Reddy R., Ro-Choi T. S., Henning D., Busch H. Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells. J Biol Chem. 1974 Oct 25;249(20):6486–6494. [PubMed] [Google Scholar]
  17. Ro-Choi T. S., Choi Y. C., Henning D., McCloskey J., Busch H. Nucleotide sequence of U-2 ribonucleic acid. The sequence of the 5'-terminal oligonucleotide. J Biol Chem. 1975 May 25;250(10):3921–3928. [PubMed] [Google Scholar]
  18. Sekeris C. E., Niessing J. Evidence for the existence of a structural RNA component in the nuclear ribonucleoprotein particles containing heterogeneous RNA. Biochem Biophys Res Commun. 1975 Feb 3;62(3):642–650. doi: 10.1016/0006-291x(75)90447-7. [DOI] [PubMed] [Google Scholar]
  19. Sonenshein G. E., Brawerman G. Entry of mRNA into polyribosomes during recovery from starvation in mouse sarcoma 180 cells. Eur J Biochem. 1977 Feb 15;73(1):307–312. doi: 10.1111/j.1432-1033.1977.tb11320.x. [DOI] [PubMed] [Google Scholar]
  20. Tolstoshev P., Wells J. R. Nature and origins of chromatin-associated ribonucleic acid of avian reticulocytes. Biochemistry. 1974 Jan 1;13(1):103–111. doi: 10.1021/bi00698a017. [DOI] [PubMed] [Google Scholar]
  21. Weinberg R. A. Nuclear RNA metabolism. Annu Rev Biochem. 1973;42:329–354. doi: 10.1146/annurev.bi.42.070173.001553. [DOI] [PubMed] [Google Scholar]
  22. Zieve G., Penman S. Small RNA species of the HeLa cell: metabolism and subcellular localization. Cell. 1976 May;8(1):19–31. doi: 10.1016/0092-8674(76)90181-1. [DOI] [PubMed] [Google Scholar]
  23. von Heyden H. W., Zachau H. G. Characterization of RNA in fractions of calf thymus chromatin. Biochim Biophys Acta. 1971 Apr 8;232(4):651–660. doi: 10.1016/0005-2787(71)90757-x. [DOI] [PubMed] [Google Scholar]

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