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. 1977 Aug 1;74(2):358–364. doi: 10.1083/jcb.74.2.358

Binding of viral glycoprotein mRNA to endoplasmic reticulum membranes is disrupted by puromycin

PMCID: PMC2110081  PMID: 195964

Abstract

Previous studies showed that the glycoprotein (G) of vesicular stomatitis virus is synthesized in association with the endoplasmic reticulum (ER) membrane and that all G mRNA co-fractionates with ER membrane. Here, we show that treatment of infected cells with puromycin results in dissociation of G mRNA, and presumably the associated ribosomes, from the ER membrane. Even it extracts from treated cells are kept at low ionic strength (0.01 M KCl), over 80% of G mRNA is found unattached to membranes. There is no evidence for direct interaction of GmRNA with membranes; rather, the linkage apparently is mediated by the nascent G polypeptide.

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Selected References

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