Abstract
Patterns of newly synthesized proteins of Vero cells in different growth states were obtained using two-dimensional gel electrophoresis. The 240 most prevalent peptide spots were then compared. Cells in exponential growth and in the stationary phase were found to have patterns of peptide spots characteristic of their state of growth. The transition between these patterns is progressive, and the cells acquire a pattern characteristic of quiescent cells by the late exponential phase. These observations suggest that a series of modulations in gene expression occurs during the transition of growth states in animal cells that leads to the specific appearance or disappearance of certain cellular peptides.
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Selected References
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- Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
- DEMARS R. SOME STUDIES OF ENZYMES IN CULTIVATED HUMAN CELLS. Natl Cancer Inst Monogr. 1964 Apr;13:181–195. [PubMed] [Google Scholar]
- Engelhardt D. L. An inhibitor of protein synthesis in cytoplasmic extracts of density inhibited cells. J Cell Physiol. 1971 Dec;78(3):333–344. doi: 10.1002/jcp.1040780303. [DOI] [PubMed] [Google Scholar]
- Engelhardt D. L., Jen-Hao M. A serum factor requirement for the passage of cultured Vero cells through G2. J Cell Physiol. 1977 Feb;90(2):307–320. doi: 10.1002/jcp.1040900216. [DOI] [PubMed] [Google Scholar]
- Gelehrter T. D., Tomkins G. M. Control of tyrosine aminotransferase synthesis in tissue culture by a factor in serum. Proc Natl Acad Sci U S A. 1969 Oct;64(2):723–730. doi: 10.1073/pnas.64.2.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hassell J., Engelhardt D. L. Factors regulating the multiplication of animal cells in culture. Exp Cell Res. 1977 Jun;107(1):159–167. doi: 10.1016/0014-4827(77)90397-4. [DOI] [PubMed] [Google Scholar]
- Holley R. W. Control of growth of mammalian cells in cell culture. Nature. 1975 Dec 11;258(5535):487–490. doi: 10.1038/258487a0. [DOI] [PubMed] [Google Scholar]
- Ivarie R. D., O'Farrell P. H. The glucocorticoid domain: steroid-mediated changes in the rate of synthesis of rat hepatoma proteins. Cell. 1978 Jan;13(1):41–55. doi: 10.1016/0092-8674(78)90136-8. [DOI] [PubMed] [Google Scholar]
- LEVINE E. M., BECKER Y., BOONE C. W., EAGLE H. CONTACT INHIBITION, MACROMOLECULAR SYNTHESIS, AND POLYRIBOSOMES IN CULTURED HUMAN DIPLOID FIBROBLASTS. Proc Natl Acad Sci U S A. 1965 Feb;53:350–356. doi: 10.1073/pnas.53.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Priest R. E., Davies L. M. Cellular proliferation and synthesis of collagen. Lab Invest. 1969 Aug;21(2):138–142. [PubMed] [Google Scholar]
- STANNERS C. P., TILL J. E. DNA synthesis in individual L-strain mouse cells. Biochim Biophys Acta. 1960 Jan 29;37:406–419. doi: 10.1016/0006-3002(60)90496-0. [DOI] [PubMed] [Google Scholar]
- Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]