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. 1978 Jun 1;77(3):R35–R42. doi: 10.1083/jcb.77.3.r35

Recovery of surface membrane in anterior pituitary cells. Variations in traffic detected with anionic and cationic ferritin

M G Farquhar
PMCID: PMC2110137  PMID: 567224

Abstract

Cells dissociated from rat anterior pituitaries were incubated with native or cationized ferritin (CF) to trace the fate of surface membrane. Native ferritin, which did not bind to the cell surface, was taken up in small amounts by bulk-phase endocytosis and was found increasingly (over 1-2 h) concentrated in lysosomes. CF at 100-fold less concentrations bound rapidly to the cell membrane, was taken up by endocytosis in far greater amounts, and within 15-60 min was found increasingly within multiple stacked Golgi cisternae, around forming secretion granules, and within elements of GERL, as well as within lysosomes. The findings demonstrate that the fate of the tracer--and presumably also that of the surface membrane--varies with the same molecule differing only in net charge: vesicles carrying anionic ferritin (net negative charge) fuse only with elements of the lysosomal system whereas those carrying CF (net positive charge) can fuse not only with elements of the lysosomal system, but also with elements along the secretory pathway (Golgi cisternae and condensing granules) as well.

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Selected References

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  1. Abrahams S. J., Holtzman E. Secretion and endocytosis in insulin-stimulated rat adrenal medulla cells. J Cell Biol. 1973 Feb;56(2):540–558. doi: 10.1083/jcb.56.2.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Basu S. K., Anderson R. G., Goldstein J. L., Brown M. S. Metabolism of cationized lipoproteins by human fibroblasts. Biochemical and morphologic correlations. J Cell Biol. 1977 Jul;74(1):119–135. doi: 10.1083/jcb.74.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Danon D., Goldstein L., Marikovsky Y., Skutelsky E. Use of cationized ferritin as a label of negative charges on cell surfaces. J Ultrastruct Res. 1972 Mar;38(5):500–510. doi: 10.1016/0022-5320(72)90087-1. [DOI] [PubMed] [Google Scholar]
  4. Farquhar M. G., Reid J. J., Daniell L. W. Intracellular transport and packaging of prolactin: a quantitative electron microscope autoradiographic study of mammotrophs dissociated from rat pituitaries. Endocrinology. 1978 Jan;102(1):296–311. doi: 10.1210/endo-102-1-296. [DOI] [PubMed] [Google Scholar]
  5. Farquhar M. G. Secretion and crinophagy in prolactin cells. Adv Exp Med Biol. 1977;80:37–94. doi: 10.1007/978-1-4615-6675-5_3. [DOI] [PubMed] [Google Scholar]
  6. Geuze J. J., Kramer M. F. Function of coated membranes and multivesicular bodies during membrane regulation in stimulated exocrine pancreas cells. Cell Tissue Res. 1974;156(1):1–20. doi: 10.1007/BF00220098. [DOI] [PubMed] [Google Scholar]
  7. Gonatas N. K., Kim S. U., Stieber A., Avrameas S. Internalization of lectins in neuronal GERL. J Cell Biol. 1977 Apr;73(1):1–13. doi: 10.1083/jcb.73.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grinnell F., Tobleman M. Q., Hackenbrock C. R. The distribution and mobility of anionic sites on the surfaces of baby hamster kidney cells. J Cell Biol. 1975 Sep;66(3):470–479. doi: 10.1083/jcb.66.3.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herzog V., Farquhar M. G. Luminal membrane retrieved after exocytosis reaches most golgi cisternae in secretory cells. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5073–5077. doi: 10.1073/pnas.74.11.5073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hopkins C. R., Farquhar M. G. Hormone secretion by cells dissociated from rat anterior pituitaries. J Cell Biol. 1973 Nov;59(2 Pt 1):276–303. doi: 10.1083/jcb.59.2.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kalina M., Robinovitch R. Exocytosis couples to endocytosis of ferritin in parotid acinar cells from isoprenalin stimulated rats. Cell Tissue Res. 1975 Nov 12;163(3):373–382. doi: 10.1007/BF00219471. [DOI] [PubMed] [Google Scholar]
  12. Larsen B. Redistribution of polycations bound to lymphocytes. Mol Cell Biochem. 1977 Apr 12;15(2):117–123. doi: 10.1007/BF01793333. [DOI] [PubMed] [Google Scholar]
  13. Novikoff A. B., Mori M., Quintana N., Yam A. Studies of the secretory process in the mammalian exocrine pancreas. I. The condensing vacuoles. J Cell Biol. 1977 Oct;75(1):148–165. doi: 10.1083/jcb.75.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oliver C., Hand A. R. Uptake and fate of luminally administered horseradish peroxidase in resting and isoproterenol-stimulated rat parotid acinar cells. J Cell Biol. 1978 Jan;76(1):207–229. doi: 10.1083/jcb.76.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pelletier G. Secretion and uptake of peroxidase by rat adenohypophyseal cells. J Ultrastruct Res. 1973 Jun;43(5):445–459. doi: 10.1016/s0022-5320(73)90021-x. [DOI] [PubMed] [Google Scholar]
  16. Quinton P. M., Philpott C. W. A role for anionic sites in epithelial architecture. Effects of cationic polymers on cell membrane structure. J Cell Biol. 1973 Mar;56(3):787–796. doi: 10.1083/jcb.56.3.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ryser H. J. A membrane effect of basic polymers dependent on molecular size. Nature. 1967 Aug 26;215(5104):934–936. doi: 10.1038/215934a0. [DOI] [PubMed] [Google Scholar]
  18. Seiler M. W., Venkatachalam M. A., Cotran R. S. Glomerular epithelium: structural alterations induced by polycations. Science. 1975 Aug 1;189(4200):390–393. doi: 10.1126/science.1145209. [DOI] [PubMed] [Google Scholar]
  19. Skutelsky E., Danon D. Redistribution of surface anionic sites on the luminal front of blood vessel endothelium after interaction with polycationic ligand. J Cell Biol. 1976 Oct;71(1):232–241. doi: 10.1083/jcb.71.1.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Skutelsky E., Hardy B. Regeneration of plasmalemma and surface properties in macrophages. Exp Cell Res. 1976 Sep;101(2):337–345. doi: 10.1016/0014-4827(76)90386-4. [DOI] [PubMed] [Google Scholar]
  21. Tixier-Vidal A., Picart R., Moreau M. F. Endocytose et sécrétion dans les cellules antéhypophysaires en culture. Action des hormones hypothalamiques. J Microsc Biol Cell. 1976 Mar;25(2):159–172. [PubMed] [Google Scholar]
  22. Wolff J., Cook G. H. Simulation of hormone effects by polycations. Endocrinology. 1977 Dec;101(6):1767–1775. doi: 10.1210/endo-101-6-1767. [DOI] [PubMed] [Google Scholar]

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