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. 1978 Nov 1;79(2):533–545. doi: 10.1083/jcb.79.2.533

Electrical coupling and uncoupling of exocrine acinar cells

PMCID: PMC2110235  PMID: 569159

Abstract

The electrical communication network in the mouse pancreatic acinar tissue has been investigated using simultaneous intracellular recording with two separate microelectrodes and direct microscopical control of the localizations of the microelectrode tips. All cells within one acinus were electrically coupled, and the coupling coefficient (the electrotonic potential change in a cell neighboring to the cell into which current is injected [V2] divided by the electrotonic potential change in the cell of current injection [V1]) between two cells near each other (less than 50 micron) was always close to 1. Cells farther apart (50-100 micron) were, in some cases, coupled; in other cases, there was no coupling at all. Coupling coefficients varied between 0 and 1. There was rarely electrical coupling over distances of more than 110 micron. Using microiontophoretic acetylcholine (ACh) application, it was possible to evoke almost complete electrical uncoupling of two previously coupled pancreatic or lacrimal acinar cells from different acini or within one acinus. The effects were fully and quickly reversible. While the ACh-evoked uncoupling in the pancreas was associated with membrane depolarization, ACh caused hyperpolarization in the lacrimal acinar cells. The uncoupling was associated with a very marked reduction in electrical time constant, indicating a reduction in input capacitance (effective surface cell membrane area). The concentrations of stimulants needed to evoke reduction in pancreatic cell-to-cell coupling were 1 micron for ACh, 0.14 nM for caerulein, and 3 nM for bombesin. These concentrations are smaller than those required to evoke maximal enzyme secretion.

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Selected References

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  1. Bolender R. P. Stereological analysis of the guinea pig pancreas. I. Analytical model and quantitative description of nonstimulated pancreatic exocrine cells. J Cell Biol. 1974 May;61(2):269–287. doi: 10.1083/jcb.61.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dean P. M., Matthews E. K. Pancreatic acinar cells: measurement of membrane potential and miniature depolarization potentials. J Physiol. 1972 Aug;225(1):1–13. doi: 10.1113/jphysiol.1972.sp009926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Friend D. S., Gilula N. B. Variations in tight and gap junctions in mammalian tissues. J Cell Biol. 1972 Jun;53(3):758–776. doi: 10.1083/jcb.53.3.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gardner J. D., Conlon T. P., Kleveman H. L., Adams T. D., Ondetti M. A. Action of cholecystokinin and cholinergic agents on calcium transport in isolated pancreatic acinar cells. J Clin Invest. 1975 Aug;56(2):366–375. doi: 10.1172/JCI108101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gilula N. B., Reeves O. R., Steinbach A. Metabolic coupling, ionic coupling and cell contacts. Nature. 1972 Feb 4;235(5336):262–265. doi: 10.1038/235262a0. [DOI] [PubMed] [Google Scholar]
  6. Ginsborg B. L., House C. R., Silinsky E. M. Conductance changes associated with the secretory potential in the cockroach salivary gland. J Physiol. 1974 Feb;236(3):723–731. doi: 10.1113/jphysiol.1974.sp010462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goodenough D. A., Revel J. P. A fine structural analysis of intercellular junctions in the mouse liver. J Cell Biol. 1970 May;45(2):272–290. doi: 10.1083/jcb.45.2.272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iwatsuki N., Petersen O. H. Membrane potential, resistance, and intercellular communication in the lacrimal gland: effects of acetylcholine and adrenaline. J Physiol. 1978 Feb;275:507–520. doi: 10.1113/jphysiol.1978.sp012204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lewis S. A., Diamond J. M. Na+ transport by rabbit urinary bladder, a tight epithelium. J Membr Biol. 1976 Aug 27;28(1):1–40. doi: 10.1007/BF01869689. [DOI] [PubMed] [Google Scholar]
  10. Matthews E. K., Petersen O. H., Williams J. A. Pancreatic acinar cells: acetylcholine-induced membrane depolarization, calcium efflux and amylase release. J Physiol. 1973 Nov;234(3):689–701. doi: 10.1113/jphysiol.1973.sp010367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Petersen O. H., Ueda N. Pancreatic acinar cells: the role of calcium in stimulus-secretion coupling. J Physiol. 1976 Jan;254(3):583–606. doi: 10.1113/jphysiol.1976.sp011248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Petersen O. H., Ueda N. Secretion of fluid and amylase in the perfused rat pancreas. J Physiol. 1977 Jan;264(3):819–835. doi: 10.1113/jphysiol.1977.sp011696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pitts J. D., Simms J. W. Permeability of junctions between animal cells. Intercellular transfer of nucleotides but not of macromolecules. Exp Cell Res. 1977 Jan;104(1):153–163. doi: 10.1016/0014-4827(77)90078-7. [DOI] [PubMed] [Google Scholar]
  14. Revel J. P., Yee A. G., Hudspeth A. J. Gap junctions between electrotonically coupled cells in tissue culture and in brown fat. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2924–2927. doi: 10.1073/pnas.68.12.2924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rose B., Simpson I., Loewenstein W. R. Calcium ion produces graded changes in permeability of membrane channels in cell junction. Nature. 1977 Jun 16;267(5612):625–627. doi: 10.1038/267625a0. [DOI] [PubMed] [Google Scholar]
  16. Scheele G. A., Palade G. E. Studies on the guinea pig pancreas. Parallel discharge of exocrine enzyme activities. J Biol Chem. 1975 Apr 10;250(7):2660–2670. [PubMed] [Google Scholar]
  17. Sheridan J. D., Hammer-Wilson M., Preus D., Johnson R. G. Quantitative analysis of low-resistance junctions between cultured cells and correlation with gap-junctional areas. J Cell Biol. 1978 Feb;76(2):532–544. doi: 10.1083/jcb.76.2.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simpson I., Rose B., Loewenstein W. R. Size limit of molecules permeating the junctional membrane channels. Science. 1977 Jan 21;195(4275):294–296. doi: 10.1126/science.831276. [DOI] [PubMed] [Google Scholar]
  19. Tsien R. W., Weingart R. Inotropic effect of cyclic AMP in calf ventricular muscle studied by a cut end method. J Physiol. 1976 Aug;260(1):117–141. doi: 10.1113/jphysiol.1976.sp011507. [DOI] [PMC free article] [PubMed] [Google Scholar]

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