Abstract
The binding of [125I]wheat germ agglutinin ([125I]WGA) of high specific activity to Chinese hamster ovary (CHO) cells has been examined over a millionfold range of WGA concentrations and correlated with the phenomena of agglutination and capping by WGA. Analysis of the binding data by the method of Scatchard gives a complex curve indicative of positive cooperativity amongst high-affinity binding sites. Binding assays performed under conditions which inhibit capping and/or agglutination, such as low temperature or glutaraldehyde fixation, give similarly complex binding curves. Thus, the gross mobility of WGA receptors in the membrane does not appear to be responsible for the cooperative binding of WGA to CHO cells.
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Selected References
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- Aubin J. E., Carlsen S. A., Ling V. Colchicine permeation is required for inhibition of concanavalin A capping in Chinese hamster ovary cells. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4516–4520. doi: 10.1073/pnas.72.11.4516. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nagata Y., Burger M. M. Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding. J Biol Chem. 1974 May 25;249(10):3116–3122. [PubMed] [Google Scholar]
- Nicolson G. L. The interactions of lectins with animal cell surfaces. Int Rev Cytol. 1974;39:89–190. doi: 10.1016/s0074-7696(08)60939-0. [DOI] [PubMed] [Google Scholar]
- Nicolson G. L. Trans-membrane control of the receptors on normal and tumor cells. II. Surface changes associated with transformation and malignancy. Biochim Biophys Acta. 1976 Apr 30;458(1):1–72. doi: 10.1016/0304-419x(76)90014-7. [DOI] [PubMed] [Google Scholar]
- Noonan K. D., Levine A. J., Burger M. M. Cell cycle-dependent changes in the surface membrane as detected with (3H)concanavalin A. J Cell Biol. 1973 Aug;58(2):491–497. doi: 10.1083/jcb.58.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanley P., Carver J. P. Selective loss of wheat germ agglutinin binding to agglutinin-resistant mutants of Chinese hamster ovary cells. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5056–5059. doi: 10.1073/pnas.74.11.5056. [DOI] [PMC free article] [PubMed] [Google Scholar]