Abstract
Antisera raised against vegetative and gametic flagella of Chlamydomonas reinhardi have been used to probe dynamic properties of the flagellar membranes. The antisera, which agglutinate cells via their flagella, associate with antigens that are present on both vegetative and gametic membranes and on membranes of both mating types (mt+ and mt-). Gametic cells respond to antibody presentation very differently from vegetative cells, mobilizing even high concentrations of antibody towards the flagellar tips; the possibility is discussed that such "tipping" ability reflects a differentiated gametic property relevant to sexual agglutinability. Gametic cells also respond to antibody agglutination by activating their mating structures, the mt+ reaction involving a rapid polymerization of microfilaments. Several impotent mt+ mutant strains that fail to agglutinate sexually are also activated by the antisera and procede to form zygotes with normal mt- gametes. Fusion does not occur between activated cells of like mating type. Monovalent (Fab) preparations of the antibody fail to activate mt+ gametes, suggesting that the cross-linking properties of the antisera are essential for their ability to mimic, or bypass, sexual agglutination.
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Selected References
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- Forest C. L., Goodenough D. A., Goodenough U. W. Flagellar membrane agglutination and sexual signaling in the conditional GAM-1 mutant of Chlamydomonas. J Cell Biol. 1978 Oct;79(1):74–84. doi: 10.1083/jcb.79.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodenough U. W., Hwang C., Warren A. J. Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics. 1978 Jun;89(2):235–243. doi: 10.1093/genetics/89.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
- Rosenbaum J. L., Moulder J. E., Ringo D. L. Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins. J Cell Biol. 1969 May;41(2):600–619. doi: 10.1083/jcb.41.2.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutishauser U., Sachs L. Receptor mobility and the mechanism of cell-cell binding induced by concanavalin A. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2456–2460. doi: 10.1073/pnas.71.6.2456. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith G. M., Regnery D. C. Inheritance of Sexuality in Chlamydomonas Reinhardi. Proc Natl Acad Sci U S A. 1950 Apr;36(4):246–248. doi: 10.1073/pnas.36.4.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snell W. J. Mating in Chlamydomonas: a system for the study of specific cell adhesion. II. A radioactive flagella-binding assay for quantitation of adhesion. J Cell Biol. 1976 Jan;68(1):70–79. doi: 10.1083/jcb.68.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss R. L., Goodenough D. A., Goodenough U. W. Membrane differentiations at sites specialized for cell fusion. J Cell Biol. 1977 Jan;72(1):144–160. doi: 10.1083/jcb.72.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss R. L., Goodenough D. A., Goodenough U. W. Membrane particle arrays associated with the basal body and with contractile vacuole secretion in Chlamydomonas. J Cell Biol. 1977 Jan;72(1):133–143. doi: 10.1083/jcb.72.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wiese L., Metz C. B. On the trypsin sensitivity of gamete contact at fertilization as studied with living gametes in Chlamydomonas. Biol Bull. 1969 Jun;136(3):483–493. doi: 10.2307/1539690. [DOI] [PubMed] [Google Scholar]