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. 1988 Apr;170(4):1768–1774. doi: 10.1128/jb.170.4.1768-1774.1988

Motility and chemotaxis of Spirochaeta aurantia: computer-assisted motion analysis.

K Fosnaugh 1, E P Greenberg 1
PMCID: PMC211029  PMID: 3350790

Abstract

A computer program has been designed to study behavior in populations of Spirochaeta aurantia cells, and this program has been used to analyze changes in behavior in response to chemoattractants. Three kinds of behavior were distinguished: smooth swimming, flexing, and reversals in direction of swimming after a short pause (120 ms). Cell populations exposed to chemoattractants spent, on average, 66, 33, and 1% of the time in these modes, respectively. After the addition of a chemoattractant, behavior was modified transiently--smooth swimming increased, flexing decreased, and reversals were suppressed. After addition of D-xylose (final concentration, 10 mM), the adaptation time (the time required for the populations to return to the unmodified behavior) for S. aurantia was 1.5 to 2.0 min. A model to explain the behavior of S. aurantia and the response of cells to chemoattractants is described. This model includes a coordinating mechanism for flagellar motor operation and a motor switch synchronizing device.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
  2. Berg H. C., Brown D. A. Chemotaxis in Escherichia coli analysed by three-dimensional tracking. Nature. 1972 Oct 27;239(5374):500–504. doi: 10.1038/239500a0. [DOI] [PubMed] [Google Scholar]
  3. Berg H. C. How spirochetes may swim. J Theor Biol. 1976 Feb;56(2):269–273. doi: 10.1016/s0022-5193(76)80074-4. [DOI] [PubMed] [Google Scholar]
  4. Berg H. C., Tedesco P. M. Transient response to chemotactic stimuli in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3235–3239. doi: 10.1073/pnas.72.8.3235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Block S. M., Segall J. E., Berg H. C. Impulse responses in bacterial chemotaxis. Cell. 1982 Nov;31(1):215–226. doi: 10.1016/0092-8674(82)90421-4. [DOI] [PubMed] [Google Scholar]
  6. Breznak J. A., Canale-Parola E. Morphology and physiology of Spirochaeta aurantia strains isolated from aquatic habitats. Arch Microbiol. 1975 Sep 30;105(1):1–12. doi: 10.1007/BF00447104. [DOI] [PubMed] [Google Scholar]
  7. Bromley D. B., Charon N. W. Axial filament involvement in the motility of Leptospira interrogans. J Bacteriol. 1979 Mar;137(3):1406–1412. doi: 10.1128/jb.137.3.1406-1412.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brown D. A., Berg H. C. Temporal stimulation of chemotaxis in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1388–1392. doi: 10.1073/pnas.71.4.1388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Canale-Parola E. Motility and chemotaxis of spirochetes. Annu Rev Microbiol. 1978;32:69–99. doi: 10.1146/annurev.mi.32.100178.000441. [DOI] [PubMed] [Google Scholar]
  10. Canale-Parola E. Physiology and evolution of spirochetes. Bacteriol Rev. 1977 Mar;41(1):181–204. doi: 10.1128/br.41.1.181-204.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Charon N. W., Daughtry G. R., McCuskey R. S., Franz G. N. Microcinematographic analysis of tethered Leptospira illini. J Bacteriol. 1984 Dec;160(3):1067–1073. doi: 10.1128/jb.160.3.1067-1073.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldman D. J., Ordal G. W. Sensory adaptation and deadaptation by Bacillus subtilis. J Bacteriol. 1981 Jul;147(1):267–270. doi: 10.1128/jb.147.1.267-270.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goulbourne E. A., Jr, Greenberg E. P. A voltage clamp inhibits chemotaxis of Spirochaeta aurantia. J Bacteriol. 1983 Feb;153(2):916–920. doi: 10.1128/jb.153.2.916-920.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Goulbourne E. A., Jr, Greenberg E. P. Chemotaxis of Spirochaeta aurantia: involvement of membrane potential in chemosensory signal transduction. J Bacteriol. 1981 Dec;148(3):837–844. doi: 10.1128/jb.148.3.837-844.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Goulbourne E. A., Jr, Greenberg E. P. Relationship between proton motive force and motility in Spirochaeta aurantia. J Bacteriol. 1980 Sep;143(3):1450–1457. doi: 10.1128/jb.143.3.1450-1457.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Greenberg E. P., Canale-Parola E. Chemotaxis in Spirochaeta aurantia. J Bacteriol. 1977 Apr;130(1):485–494. doi: 10.1128/jb.130.1.485-494.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Holt S. C. Anatomy and chemistry of spirochetes. Microbiol Rev. 1978 Mar;42(1):114–160. doi: 10.1128/mr.42.1.114-160.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Larsen S. H., Reader R. W., Kort E. N., Tso W. W., Adler J. Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli. Nature. 1974 May 3;249(452):74–77. doi: 10.1038/249074a0. [DOI] [PubMed] [Google Scholar]
  19. Macnab R. M. Bacterial flagella rotating in bundles: a study in helical geometry. Proc Natl Acad Sci U S A. 1977 Jan;74(1):221–225. doi: 10.1073/pnas.74.1.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Macnab R. M., Han D. P. Asynchronous switching of flagellar motors on a single bacterial cell. Cell. 1983 Jan;32(1):109–117. doi: 10.1016/0092-8674(83)90501-9. [DOI] [PubMed] [Google Scholar]
  21. Macnab R. M., Koshland D. E., Jr The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509–2512. doi: 10.1073/pnas.69.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Margolin Y., Eisenbach M. Voltage clamp effects on bacterial chemotaxis. J Bacteriol. 1984 Aug;159(2):605–610. doi: 10.1128/jb.159.2.605-610.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ravid S., Matsumura P., Eisenbach M. Restoration of flagellar clockwise rotation in bacterial envelopes by insertion of the chemotaxis protein CheY. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7157–7161. doi: 10.1073/pnas.83.19.7157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Segall J. E., Ishihara A., Berg H. C. Chemotactic signaling in filamentous cells of Escherichia coli. J Bacteriol. 1985 Jan;161(1):51–59. doi: 10.1128/jb.161.1.51-59.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Segall J. E., Manson M. D., Berg H. C. Signal processing times in bacterial chemotaxis. Nature. 1982 Apr 29;296(5860):855–857. doi: 10.1038/296855a0. [DOI] [PubMed] [Google Scholar]
  26. Spudich J. L., Koshland D. E., Jr Quantitation of the sensory response in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1975 Feb;72(2):710–713. doi: 10.1073/pnas.72.2.710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sundberg S. A., Bogomolni R. A., Spudich J. L. Selection and properties of phototaxis-deficient mutants of Halobacterium halobium. J Bacteriol. 1985 Oct;164(1):282–287. doi: 10.1128/jb.164.1.282-287.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wolfe A. J., Conley M. P., Kramer T. J., Berg H. C. Reconstitution of signaling in bacterial chemotaxis. J Bacteriol. 1987 May;169(5):1878–1885. doi: 10.1128/jb.169.5.1878-1885.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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