Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1979 Jan 1;80(1):118–127. doi: 10.1083/jcb.80.1.118

Fibronectin expression is determined by the genotype of the transformed parental cells in heterokaryons between normal and transformed fibroblasts

PMCID: PMC2110290  PMID: 422645

Abstract

The expression of fibronectin, a cell surface-associated transformation- sensitive glycoprotein, was studied in hetero- and homokaryons of normal and SV40-transformed human fibroblasts. In immunofluorescence, fibroblast homokaryons had an intense surface-associated and intracelluar fibronectin fluorescence similar to that of normal fibroblasts. Transformed cells and their homokaryons had a minimal surface-associated and a weak intracellular fibronectin fluorescence. In heterokaryons formed between transformed and normal fibroblasts, the expression of fibronectin fell within 24 h to the level of the transformed cell homokaryons. The change was detectable already at 3 h after fusion and was gene-dose dependent. These results show that the transformed genotype determines fibronectin expression in the heterokaryons.

Full Text

The Full Text of this article is available as a PDF (2.6 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gordon S., Cohn Z. Macrophage-melanoma cell heterokaryons. 3. The activation of macrophage DNA synthesis. Studies with inhibitors of protein synthesis and with synchronized melanoma cells. J Exp Med. 1971 Oct 1;134(4):935–946. doi: 10.1084/jem.134.4.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Harris H., Wiener F., Klein G. The analysis of malignancy by cell fusion. 3. Hybrids between diploid fibroblasts and other tumour cells. J Cell Sci. 1971 May;8(3):681–692. doi: 10.1242/jcs.8.3.681. [DOI] [PubMed] [Google Scholar]
  3. Hedman K., Vaheri A., Wartiovaara J. External fibronectin of cultured human fibroblasts is predominantly a matrix protein. J Cell Biol. 1978 Mar;76(3):748–760. doi: 10.1083/jcb.76.3.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hynes R. O. Alteration of cell-surface proteins by viral transformation and by proteolysis. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170–3174. doi: 10.1073/pnas.70.11.3170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hynes R. O., Bye J. M. Density and cell cycle dependence of cell surface proteins in hamster fibroblasts. Cell. 1974 Oct;3(2):113–120. doi: 10.1016/0092-8674(74)90114-7. [DOI] [PubMed] [Google Scholar]
  6. Klein G., Bregula U., Wiener F., Harris H. The analysis of malignancy by cell fusion. I. Hybrids between tumour cells and L cell derivatives. J Cell Sci. 1971 May;8(3):659–672. doi: 10.1242/jcs.8.3.659. [DOI] [PubMed] [Google Scholar]
  7. Koprowski H., Croce C. M. Tumorigenicity of simian virus 40-transformed human cells and mouse--human hybrids in nude mice. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1142–1146. doi: 10.1073/pnas.74.3.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller C. L., Fuseler J. W., Brinkley B. R. Cytoplasmic microtubules in transformed mouse x nontransformed human cell hybrids: correlation with in vitro growth. Cell. 1977 Sep;12(1):319–331. doi: 10.1016/0092-8674(77)90210-0. [DOI] [PubMed] [Google Scholar]
  9. Ringertz N. R., Carlsson S. A., Ege T., Bolund L. Detection of human and chick nuclear antigens in nuclei of chick erythrocytes during reactivation in heterokaryons with HeLa cells. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3228–3232. doi: 10.1073/pnas.68.12.3228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Steplewski Z., Knowles B. B., Koprowski H. The mechanism of internuclear transmission of SV40-induced complement fixation antigen in heterokaryocytes. Proc Natl Acad Sci U S A. 1968 Mar;59(3):769–776. doi: 10.1073/pnas.59.3.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vaheri A., Ruoslahti E. Disappearance of a major cell-type specific surface glycoprotein antigen (SF) after transformation of fibroblasts by Rous sarcoma virus. Int J Cancer. 1974 May 15;13(5):579–586. doi: 10.1002/ijc.2910130502. [DOI] [PubMed] [Google Scholar]
  12. Veomett G., Prescott D. M., Shay J., Porter K. R. Reconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B. Proc Natl Acad Sci U S A. 1974 May;71(5):1999–2002. doi: 10.1073/pnas.71.5.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wartiovaara J., Leivo I., Virtanen I., Vaheri A., Graham C. F. Appearance of fibronectin during differentiation of mouse teratocarcinoma in vitro. Nature. 1978 Mar 23;272(5651):355–356. doi: 10.1038/272355a0. [DOI] [PubMed] [Google Scholar]
  14. Weiss M. C., Ephrussi B. Studies of Interspecific (Rat x Mouse) Somatic Hybrids. I. Isolation, Growth and Evolution of the Karyotype. Genetics. 1966 Nov;54(5):1095–1109. doi: 10.1093/genetics/54.5.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiss M. C. Further studies on loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells. Proc Natl Acad Sci U S A. 1970 May;66(1):79–86. doi: 10.1073/pnas.66.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wiblin C. N., Macpherson I. Reversion in hybrids between SV40-transformed hamster and mouse cells. Int J Cancer. 1973 Jul 15;12(1):148–161. doi: 10.1002/ijc.2910120116. [DOI] [PubMed] [Google Scholar]
  17. Wiener F., Klein G., Harris H. The analysis of malignancy by cell fusion. V. Further evidence of the ability of normal diploid cells to suppress malignancy. J Cell Sci. 1974 Jun;15(1):177–183. doi: 10.1242/jcs.15.1.177. [DOI] [PubMed] [Google Scholar]
  18. Wiener F., Klein G., Harris H. The analysis of malignancy by cell fusion. VI. Hybrids between different tumour cells. J Cell Sci. 1974 Oct;16(1):189–198. doi: 10.1242/jcs.16.1.189. [DOI] [PubMed] [Google Scholar]
  19. Yamada K. M., Yamada S. S., Pastan I. Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1217–1221. doi: 10.1073/pnas.73.4.1217. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES