Skip to main content
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1979 May 1;81(2):348–354. doi: 10.1083/jcb.81.2.348

Cytochalasin B inhibition of endothelial proliferation at wound edges in vitro

PMCID: PMC2110320  PMID: 468908

Abstract

Cytochalasin B prevents both migration and wound-associated replication when applied to wounded monolayers of bovine endothelium in tissue culture. The normal low background rate of replication in undisturbed areas of the cultures is not inhibited by cytochalasin B. These results suggest that some form of movement may be required for initiation of wound-associated replication by endothelial cells.

Full Text

The Full Text of this article is available as a PDF (836.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bürk R. R. A factor from a transformed cell line that affects cell migration. Proc Natl Acad Sci U S A. 1973 Feb;70(2):369–372. doi: 10.1073/pnas.70.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Castor L. N. Control of division by cell contact and serum concentration in cultures of 3T3 cells. Exp Cell Res. 1971 Sep;68(1):17–24. doi: 10.1016/0014-4827(71)90581-7. [DOI] [PubMed] [Google Scholar]
  3. Davies P. F., Ross R. Mediation of pinocytosis in cultured arterial smooth muscle and endothelial cells by platelet-derived growth factor. J Cell Biol. 1978 Dec;79(3):663–671. doi: 10.1083/jcb.79.3.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Folkman J., Moscona A. Role of cell shape in growth control. Nature. 1978 Jun 1;273(5661):345–349. doi: 10.1038/273345a0. [DOI] [PubMed] [Google Scholar]
  5. Gail M. H., Scher C. D., Boone C. W. Dissociation of cell motility from cell proliferation in BALB-c-3T3 fibroblasts. Exp Cell Res. 1972 Feb;70(2):439–443. doi: 10.1016/0014-4827(72)90158-9. [DOI] [PubMed] [Google Scholar]
  6. Gurney T., Jr Local stimulation of growth in primary cultures of chick embryo fibroblasts. Proc Natl Acad Sci U S A. 1969 Mar;62(3):906–911. doi: 10.1073/pnas.62.3.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holley R. W., Kiernan J. A. "Contact inhibition" of cell division in 3T3 cells. Proc Natl Acad Sci U S A. 1968 May;60(1):300–304. doi: 10.1073/pnas.60.1.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kopriwa B M. A semiautomatic instrument for the radioautographic coating technique. J Histochem Cytochem. 1966 Dec;14(12):923–928. doi: 10.1177/14.12.923. [DOI] [PubMed] [Google Scholar]
  9. Lipton A., Klinger I., Paul D., Holley R. W. Migration of mouse 3T3 fibroblasts in response to a serum factor. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2799–2801. doi: 10.1073/pnas.68.11.2799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mizel S. B., Wilson L. Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B. J Biol Chem. 1972 Jun 25;247(12):4102–4105. [PubMed] [Google Scholar]
  11. Nicolson G. L. Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components. Biochim Biophys Acta. 1976 Apr 13;457(1):57–108. doi: 10.1016/0304-4157(76)90014-9. [DOI] [PubMed] [Google Scholar]
  12. Ross R., Harker L. Hyperlipidemia and atherosclerosis. Science. 1976 Sep 17;193(4258):1094–1100. doi: 10.1126/science.822515. [DOI] [PubMed] [Google Scholar]
  13. Schwartz S. M., Benditt E. P. Cell replication in the aortic endothelium: a new method for study of the problem. Lab Invest. 1973 Jun;28(6):699–707. [PubMed] [Google Scholar]
  14. Schwartz S. M., Haudenschild C. C., Eddy E. M. Endothelial regneration. I. Quantitative analysis of initial stages of endothelial regeneration in rat aortic intima. Lab Invest. 1978 May;38(5):568–580. [PubMed] [Google Scholar]
  15. Sholley M. M., Gimbrone M. A., Jr, Cotran R. S. Cellular migration and replication in endothelial regeneration: a study using irradiated endothelial cultures. Lab Invest. 1977 Jan;36(1):18–25. [PubMed] [Google Scholar]
  16. TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]
  18. Vasiliev J. M., Gelfand I. M., Domnina L. V., Rappoport R. I. Wound healing processes in cell cultures. Exp Cell Res. 1969 Jan;54(1):83–93. doi: 10.1016/0014-4827(69)90296-1. [DOI] [PubMed] [Google Scholar]
  19. Whittenberger B., Glaser L. Inhibition of DNA synthesis in cultures of 3T3 cells by isolated surface membranes. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2251–2255. doi: 10.1073/pnas.74.6.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yarnell M. M., Schnebli H. P. Release from density-dependent inhibition of growth in the absence of cell locomotion. J Cell Sci. 1974 Oct;16(1):181–188. doi: 10.1242/jcs.16.1.181. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES