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. 1979 Jun 1;81(3):665–671. doi: 10.1083/jcb.81.3.665

Interaction of microtubule proteins with phospholipid vesicles

PMCID: PMC2110388  PMID: 457778

Abstract

We have examined the interaction of unilamellar dimyristoyl phosphatidylcholine liposomes with the high-speed supernate of brain homogenate and with tubulin purified through one or two cycles of microtubule assembly-disassembly. Tubulin and certian high molecular weight proteins are selectively adsorbed from these mixtures onto liposomes. The composition of adsorbed proteins is similar to that obtained during corresponding cycles of microtubule assembly, suggesting the equivalency of these processes. Adsorption induces stacking and/or fusion of liposomes into multilamellar structures indicating strong protein-lipid interaction. In addition, liposome- adsorbed tubulin forms extensive intermolecular disulfide bridges that are inert to reducing agents in the aqueous medium. The observations form a basis for further study of the distribution, function, and properties of membrane-bound tubulin.

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Selected References

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  1. Albertini D. F., Berlin R. D., Oliver J. M. The mechanism of concanavalin A cap formation in leukocytes. J Cell Sci. 1977 Aug;26:57–75. doi: 10.1242/jcs.26.1.57. [DOI] [PubMed] [Google Scholar]
  2. Becker J. S., Oliver J. M., Berlin R. D. Fluorescence techniques for following interactions of microtubule subunits and membranes. Nature. 1975 Mar 13;254(5496):152–154. doi: 10.1038/254152a0. [DOI] [PubMed] [Google Scholar]
  3. Berlin R. D., Fera J. P. Changes in membrane microviscosity associated with phagocytosis: effects of colchicine. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1072–1076. doi: 10.1073/pnas.74.3.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berlin R. D., Oliver J. M. Analogous ultrastructure and surface properties during capping and phagocytosis in leukocytes. J Cell Biol. 1978 Jun;77(3):789–804. doi: 10.1083/jcb.77.3.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blitz A. L., Fine R. E. Muscle-like contractile proteins and tubulin in synaptosomes. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4472–4476. doi: 10.1073/pnas.71.11.4472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAS M. L., HAAK E. D., CRANE F. L. PROTEOLIPIDS. IV. FORMATION OF COMPLEXES BETWEEN CYTOCHROME C AND PURIFIED PHOSPHOLIPIDS. Biochemistry. 1965 May;4:859–865. doi: 10.1021/bi00881a010. [DOI] [PubMed] [Google Scholar]
  7. Eytan G. D., Matheson M. J., Racker E. Incorporation of mitochondrial membrane proteins into liposomes containing acidic phospholipids. J Biol Chem. 1976 Nov 10;251(21):6831–6837. [PubMed] [Google Scholar]
  8. Feit H., Barondes S. H. Colchicine-binding activity in particulate fractions of mouse brain. J Neurochem. 1970 Sep;17(9):1355–1364. doi: 10.1111/j.1471-4159.1970.tb06870.x. [DOI] [PubMed] [Google Scholar]
  9. Feit H., Dutton G. R., Barondes S. H., Shelanski M. L. Microtubule protein. Identification in and transport to nerve endings. J Cell Biol. 1971 Oct;51(1):138–147. doi: 10.1083/jcb.51.1.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gerber G. E., Gray C. P., Wildenauer D., Khorana H. G. Orientation of bacteriorhodopsin in Halobacterium halobium as studied by selective proteolysis. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5426–5430. doi: 10.1073/pnas.74.12.5426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gulik-Krzywicki T., Shechter E., Vittorio Luzzati, Faure M. Interactions of proteins and lipids: structure and polymorphism of protein-lipid-water phases. Nature. 1969 Sep 13;223(5211):1116–1121. doi: 10.1038/2231116a0. [DOI] [PubMed] [Google Scholar]
  12. Kelly P. T., Cotman C. W. Intermolecular disulfide bonds at central nervous system synaptic junctions. Biochem Biophys Res Commun. 1976 Dec 20;73(4):858–864. doi: 10.1016/0006-291x(76)90200-x. [DOI] [PubMed] [Google Scholar]
  13. Kelly P. T., Cotman C. W. Synaptic proteins. Characterization of tubulin and actin and identification of a distinct postsynaptic density polypeptide. J Cell Biol. 1978 Oct;79(1):173–183. doi: 10.1083/jcb.79.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kimelberg H. K., Papahadjopoulos D. Interactions of basic proteins with phospholipid membranes. Binding and changes in the sodium permeability of phosphatidylserine vesicles. J Biol Chem. 1971 Feb 25;246(4):1142–1148. [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Margolis R. L., Wilson L. Opposite end assembly and disassembly of microtubules at steady state in vitro. Cell. 1978 Jan;13(1):1–8. doi: 10.1016/0092-8674(78)90132-0. [DOI] [PubMed] [Google Scholar]
  17. Oliver J. M. Impaired microtubule function correctable by cyclic GMP and cholinergic agonists in the Chediak-Higashi syndrome. Am J Pathol. 1976 Nov;85(2):395–418. [PMC free article] [PubMed] [Google Scholar]
  18. Oliver J. M., Ukena T. E., Berlin R. D. Effects of phagocytosis and colchicine on the distribution of lectin-binding sites on cell surfaces. Proc Natl Acad Sci U S A. 1974 Feb;71(2):394–398. doi: 10.1073/pnas.71.2.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reynolds J. A., Tanford C. Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes. Proc Natl Acad Sci U S A. 1970 Jul;66(3):1002–1007. doi: 10.1073/pnas.66.3.1002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rogers M. J., Strittmatter P. The interaction of NADH-cytochrome b5 reductase and cytochrome b5 bound to egg lecithin liposomes. J Biol Chem. 1975 Jul 25;250(14):5713–5718. [PubMed] [Google Scholar]
  21. Sweet C., Zull J. E. The binding of serum albumin to phospholipid liposomes. Biochim Biophys Acta. 1970 Dec 1;219(2):253–262. doi: 10.1016/0005-2736(70)90204-x. [DOI] [PubMed] [Google Scholar]
  22. Ukena T. E., Berlin R. D. Effect of colchicine and vinblastine on the topographical separation of membrane functions. J Exp Med. 1972 Jul 1;136(1):1–7. doi: 10.1084/jem.136.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weingarten M. D., Lockwood A. H., Hwo S. Y., Kirschner M. W. A protein factor essential for microtubule assembly. Proc Natl Acad Sci U S A. 1975 May;72(5):1858–1862. doi: 10.1073/pnas.72.5.1858. [DOI] [PMC free article] [PubMed] [Google Scholar]

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