Abstract
The kinetics of Ca++ uptake have been evaluated in 3T3 and SV40-3T3 mouse cells. The data reveal at least two exchangeable cellular compartments in the 3T3 and SV40-3T3 cell over a 50-min exposure to 45Ca++. A rapidly exchanging compartment may represent surface-membrane- localized Ca++ whereas a more slowly exchanging compartment is presumably intracellular. The transition of the 3T3 cell from exponential growth (at 3 day's incubation) to quiescence (at 7 days) is characterized by a 7.5-fold increase in the size of the fast component. Quiescence of the 3T3 cell is also characterized by a 3.2-fold increase in the unidirectional Ca++ influx into the slowly exchanging compartment and a 3.6-fold increase in its size. The increase in size of the slow compartment at quiescence may result from a redistribution of intracellular Ca++ to a more readily exchangeable compartment, possibly reflecting a release of previously bound Ca++. In contrast, no significant change in any of these parameters is observed in the proliferatively active SV40-3T3 cells after corresponding period of incubation, even though these cells attained higher growth densities and underwent postconfluence.
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Selected References
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- Baker M. E. Colchicine inhibits mitogenesis in C1300 neuroblastoma cells that have been arrested in G0. Nature. 1976 Aug 26;262(5571):785–786. doi: 10.1038/262785a0. [DOI] [PubMed] [Google Scholar]
- Borle A. B. Calcium metabolism at the cellular level. Fed Proc. 1973 Sep;32(9):1944–1950. [PubMed] [Google Scholar]
- Borle A. B. Kinetic analyses of calcium movements in HeLa cell cultures. I. Calcium influx. J Gen Physiol. 1969 Jan;53(1):43–56. doi: 10.1085/jgp.53.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boynton A. L., Whitfield J. F., Isaacs R. J. The different roles of serum and calcium in the control of proliferation of BALB/c 3T3 mouse cells. In Vitro. 1976 Feb;12(2):120–123. doi: 10.1007/BF02796358. [DOI] [PubMed] [Google Scholar]
- Gunther G. R., Wang J. L., Edelman G. M. Kinetics of colchicine inhibition of mitogenesis in individual lymphocytes. Exp Cell Res. 1976 Mar 1;98(1):15–22. doi: 10.1016/0014-4827(76)90457-2. [DOI] [PubMed] [Google Scholar]
- Kondo S., Schulz I. Ca++ fluxes in isolated cells of rat pancreas. effect of secretagogues and different Ca++ concentrations. J Membr Biol. 1976 Oct 20;29(1-2):185–203. doi: 10.1007/BF01868959. [DOI] [PubMed] [Google Scholar]
- Moore L., Pastan I. Energy-dependent calcium uptake activity in cultured mouse fibroblast microsomes. Regulation of the uptake system by cell density. J Biol Chem. 1977 Sep 25;252(18):6304–6309. [PubMed] [Google Scholar]
- Rubin H., Koide T. Mutual potentiation by magnesium and calcium of growth in animal cells. Proc Natl Acad Sci U S A. 1976 Jan;73(1):168–172. doi: 10.1073/pnas.73.1.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tupper J. T., Del Rosso M., Hazelton B., Zorgniotti F. Serum-stimulated changes in calcium transport and distribution in mouse 3T3 cells and their modification by dibutyryl cyclic AMP. J Cell Physiol. 1978 Apr;95(1):71–84. doi: 10.1002/jcp.1040950110. [DOI] [PubMed] [Google Scholar]
- Tupper J. T., Zorgniotti F. Calcium content and distribution as a function of growth and transformation in the mouse 3T3 cell. J Cell Biol. 1977 Oct;75(1):12–22. doi: 10.1083/jcb.75.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vannucchi S., Del Rosso M., Cella C., Urbano P., Chiarugi V. Surface glycosaminoglycans and calcium distribution in 3T3 cells. Biochem J. 1978 Jan 15;170(1):185–187. doi: 10.1042/bj1700185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whitfield J. F., MacManus J. P., Rixon R. H., Boynton A. L., Youdale T., Swierenga S. The positive control of cell proliferation by the interplay on calcium ions and cyclic nucleotides. A review. In Vitro. 1976 Jan;12(1):1–18. doi: 10.1007/BF02832787. [DOI] [PubMed] [Google Scholar]