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. 1979 Oct 1;83(1):249–254. doi: 10.1083/jcb.83.1.249

High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements

PMCID: PMC2110435  PMID: 574514

Abstract

Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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