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. 1979 Sep 1;82(3):829–833. doi: 10.1083/jcb.82.3.829

Capping of exogenous Forssman glycolipid on cells

PMCID: PMC2110488  PMID: 389939

Abstract

When motile cells are incubated with Forssman glycolipid, the antigen is incorporated into the cells' plasma membranes. If cross-linked by antibody, the patched glycolipids cap. This process is sensitive to those drugs that are known to inhibit capping of protein antigens. The results support a flow mechanism for capping.

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Selected References

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  1. Abercrombie M., Heaysman J. E., Pegrum S. M. The locomotion of fibroblasts in culture. 3. Movements of particles on the dorsal surface of the leading lamella. Exp Cell Res. 1970 Oct;62(2):389–398. doi: 10.1016/0014-4827(70)90570-7. [DOI] [PubMed] [Google Scholar]
  2. Bourguignon L. Y., Singer S. J. Transmembrane interactions and the mechanism of capping of surface receptors by their specific ligands. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5031–5035. doi: 10.1073/pnas.74.11.5031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bretscher M. S. Directed lipid flow in cell membranes. Nature. 1976 Mar 4;260(5546):21–23. doi: 10.1038/260021a0. [DOI] [PubMed] [Google Scholar]
  4. Craig S. W., Cuatrecasas P. Mobility of cholera toxin receptors on rat lymphocyte membranes. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3844–3848. doi: 10.1073/pnas.72.10.3844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fraser B. A., Mallette M. F. An improved isolation method and new composition data for Forssman hapten from sheep erythrocytes. Immunochemistry. 1973 Nov;10(11):745–753. doi: 10.1016/0019-2791(73)90176-6. [DOI] [PubMed] [Google Scholar]
  6. Révész T., Greaves M. Ligand-induced redistribution of lymphocyte membrane ganglioside GM1. Nature. 1975 Sep 11;257(5522):103–106. doi: 10.1038/257103a0. [DOI] [PubMed] [Google Scholar]
  7. Stern P. L., Willison K. R., Lennox E., Galfrè G., Milstein C., Secher D., Ziegler A. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell. 1978 Aug;14(4):775–783. doi: 10.1016/0092-8674(78)90333-1. [DOI] [PubMed] [Google Scholar]
  8. Ziolkowski C. H., Fraser B. A., Mallette M. F. Sheep erythrocyte Forssman hapten, an isohapten system: composition of the ceramide. Immunochemistry. 1975 Apr;12(4):297–302. doi: 10.1016/0019-2791(75)90179-2. [DOI] [PubMed] [Google Scholar]
  9. de Petris S. Nonuniform distribution of concanavalin-A receptors and surface antigens on uropod-forming thymocytes. J Cell Biol. 1978 Oct;79(1):235–251. doi: 10.1083/jcb.79.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. de Petris S., Raff M. C. Normal distribution, patching and capping of lymphocyte surface immunoglobulin studied by electron microscopy. Nat New Biol. 1973 Feb 28;241(113):257–259. doi: 10.1038/newbio241257a0. [DOI] [PubMed] [Google Scholar]

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