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. 1980 Feb 1;84(2):261–280. doi: 10.1083/jcb.84.2.261

Rows of dimeric-particles within the axolemma and juxtaposed particles within glia, incorporated into a new model for the paranodal glial- axonal junction at the node of Ranvier

PMCID: PMC2110539  PMID: 7380883

Abstract

Using freeze-fracture techniques, we have analyzed the glial-axonal junction (GAJ) between Schwann cells and axons in the peripheral nervous system, and between oligodendrocytes and axons in the central nervous system of the rat. We have identified a new set of dimeric- particles arranged in circumferential rows within the protoplasmic fracture faces (P-faces) of the paranodal axolemma in the region of glial-axonal juxtaposition. These particles, 260 A in length, composed of two 115-A subunits, are observed in both aldehyde-fixed and nonfixed preparations. The rows of dimeric-particles within the axonal P-face are associated with complementary rows of pits within the external fracture face (E-face) of the paranodal axolemma. These axonal particles are positioned between rows of 160-A particles that occur in both fracture faces of the glial loops in the same region. We observed, in addition to these previously described 160-A particles, a new set of 75-A glial particles within the glial P-faces of the GAJ. These 75-A particles form rows that are centered between the rows of 160-A particles and are therefore superimposed over the rows of dimeric- particles within the paranodal axolemma. Our new findings are interpreted with respect to methods of specimen preparation as well as to a potential role for the paranodal organ in saltatory conduction. We conclude that this particle-rich junction between axon and glia could potentially provide an intricate mechanism for ion exchange between these two cell types.

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Selected References

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  1. ABBOTT B. C., HILL A. V., HOWARTH J. V. The positive and negative heat production associated with a nerve impulse. Proc R Soc Lond B Biol Sci. 1958 Feb 18;148(931):149–187. doi: 10.1098/rspb.1958.0012. [DOI] [PubMed] [Google Scholar]
  2. ANDRES K. H. UBER DIE FEINSTRUKTUR BESONDERER EINRICHTUNGEN IN MARKHALTIGEN NERVENFASERN DES KLEINHIRNS DER RATTE. Z Zellforsch Mikrosk Anat. 1965 Feb 24;65:701–712. [PubMed] [Google Scholar]
  3. Agnew W. S., Levinson S. R., Brabson J. S., Raftery M. A. Purification of the tetrodotoxin-binding component associated with the voltage-sensitive sodium channel from Electrophorus electricus electroplax membranes. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2606–2610. doi: 10.1073/pnas.75.6.2606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BARGMANN W., LINDNER E. UBER DEN FEINBAU DES NEBENNIERENMARKES DES IGELS (ERINACEUS EUROPAEUS L.) Z Zellforsch Mikrosk Anat. 1964 Dec 3;64:868–912. [PubMed] [Google Scholar]
  5. Blank W. F., Jr, Bunge M. B., Bunge R. P. The sensitivity of the myelin sheath, particularly the Schwann cell-axolemmal junction, to lowered calcium levels in cultured sensory ganglia. Brain Res. 1974 Mar 8;67(3):503–518. doi: 10.1016/0006-8993(74)90498-3. [DOI] [PubMed] [Google Scholar]
  6. Bunge M. B., Bunge R. P., Peterson E. R., Murray M. R. A light and electron microscope study of long-term organized cultures of rat dorsal root ganglia. J Cell Biol. 1967 Feb;32(2):439–466. doi: 10.1083/jcb.32.2.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Conti F., Hille B., Neumcke B., Nonner W., Stämpfli R. Conductance of the sodium channel in myelinated nerve fibres with modified sodium inactivation. J Physiol. 1976 Nov;262(3):729–742. doi: 10.1113/jphysiol.1976.sp011617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deguchi N., Jorgensen P. L., Maunsbach A. B. Ultrastructure of the sodium pump. Comparison of thin sectioning, negative staining, and freeze-fracture of purified, membrane-bound (Na+,K+)-ATPase. J Cell Biol. 1977 Dec;75(3):619–634. doi: 10.1083/jcb.75.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dempsey G. P., Bullivant S., Watkins W. B. Endothelial cell membranes: polarity of particles as seen by freeze-fracturing. Science. 1973 Jan 12;179(4069):190–192. doi: 10.1126/science.179.4069.190. [DOI] [PubMed] [Google Scholar]
  10. Dermietzel R. Junctions in the central nervous system of the cat. II. A contribution to the tertiary structure of the axonal-glial junctions in the paranodal region of the node of Ranvier. Cell Tissue Res. 1974 May 8;148(4):577–586. doi: 10.1007/BF00221941. [DOI] [PubMed] [Google Scholar]
  11. FRANKENHAEUSER B. [The hypothesis of saltatory conduction]. Cold Spring Harb Symp Quant Biol. 1952;17:27–36. doi: 10.1101/sqb.1952.017.01.005. [DOI] [PubMed] [Google Scholar]
  12. Gross H., Kuebler O., Bas E., Moor H. Decoration of specific sites on freeze-fractured membranes. J Cell Biol. 1978 Dec;79(3):646–656. doi: 10.1083/jcb.79.3.646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HILL A. V., HOWARTH J. V. The initial heat production of stimulated nerve. Proc R Soc Lond B Biol Sci. 1958 Dec 4;149(935):167–175. doi: 10.1098/rspb.1958.0059. [DOI] [PubMed] [Google Scholar]
  14. Hirano A., Dembitzer H. M. The transverse bands as a means of access to the periaxonal space of the central myelinated nerve fiber. J Ultrastruct Res. 1969 Jul;28(1):141–149. doi: 10.1016/s0022-5320(69)90012-4. [DOI] [PubMed] [Google Scholar]
  15. Kristol C., Akert K., Sandri C., Wyss U. R., Bennett M. V., Moor H. The Ranvier nodes in the neurogenic electric organ of the knifefish Sternarchus: a freeze-etching study on the distribution of membrane-associated particles. Brain Res. 1977 Apr 15;125(2):197–212. doi: 10.1016/0006-8993(77)90615-1. [DOI] [PubMed] [Google Scholar]
  16. Kristol C., Sandri C., Akert K. Intramembranous particles at the nodes of Ranvier of the cat spinal cord: a morphometric study. Brain Res. 1978 Mar 10;142(3):391–400. doi: 10.1016/0006-8993(78)90903-4. [DOI] [PubMed] [Google Scholar]
  17. Laatsch R. H., Cowan W. M. A structural specialization at nodes of Ranvier in the central nervous system. Nature. 1966 May 14;210(5037):757–758. doi: 10.1038/210757a0. [DOI] [PubMed] [Google Scholar]
  18. Lasansky A. Basal junctions at synaptic endings of turtle visual cells. J Cell Biol. 1969 Feb;40(2):577–581. doi: 10.1083/jcb.40.2.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Levinson S. R., Ellory J. C. Molecular size of the tetrodotoxin binding site estimated by irradiation inactivation. Nat New Biol. 1973 Sep 26;245(143):122–123. doi: 10.1038/newbio245122a0. [DOI] [PubMed] [Google Scholar]
  20. Livingston R. B., Pfenniger K., Moor H., Akert K. Specialized paranodal and interparanodal glial-axonal junctions in the peripheral and central nervous system: a freeze-etching study. Brain Res. 1973 Aug 17;58(1):1–24. doi: 10.1016/0006-8993(73)90820-2. [DOI] [PubMed] [Google Scholar]
  21. Müller-Mohnssen H., Tippe A., Hillenkamp F., Unsöld E. Is the rise of the action potential of the ranvier node controlled by a paranodal organ? Naturwissenschaften. 1974 Aug;61(8):369–370. doi: 10.1007/BF00600316. [DOI] [PubMed] [Google Scholar]
  22. Nonner W., Rojas E., Stämpfli R. Gating currents in the node of Ranvier: voltage and time dependence. Philos Trans R Soc Lond B Biol Sci. 1975 Jun 10;270(908):483–492. doi: 10.1098/rstb.1975.0024. [DOI] [PubMed] [Google Scholar]
  23. Rasminsky M., Sears T. A. Internodal conduction in undissected demyelinated nerve fibres. J Physiol. 1972 Dec;227(2):323–350. doi: 10.1113/jphysiol.1972.sp010035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ritchie J. M., Rogart R. B. Density of sodium channels in mammalian myelinated nerve fibers and nature of the axonal membrane under the myelin sheath. Proc Natl Acad Sci U S A. 1977 Jan;74(1):211–215. doi: 10.1073/pnas.74.1.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rosenbluth J. Intramembranous particle distribution at the node of Ranvier and adjacent axolemma in myelinated axons of the frog brain. J Neurocytol. 1976 Dec;5(6):731–745. doi: 10.1007/BF01181584. [DOI] [PubMed] [Google Scholar]
  26. STAMPFLI R. Saltatory conduction in nerve. Physiol Rev. 1954 Jan;34(1):101–112. doi: 10.1152/physrev.1954.34.1.101. [DOI] [PubMed] [Google Scholar]
  27. Sandri C., Van Buren J. M., Akert K. Membrane morphology of the vertebrate nervous system. A study with freeze-etch technique. Prog Brain Res. 1977;46:1–384. [PubMed] [Google Scholar]
  28. Schnapp B., Peracchia C., Mugnaini E. The paranodal axo-glial junction in the central nervous system studied with thin sections and freeze-fracture. Neuroscience. 1976 Jun;1(3):181–190. doi: 10.1016/0306-4522(76)90075-0. [DOI] [PubMed] [Google Scholar]
  29. Wood J. G., Jean D. H., Whitaker J. N., McLaughlin B. J., Albers R. W. Immunocytochemical localization of the sodium, potassium activated ATPase in knifefish brain. J Neurocytol. 1977 Oct;6(5):571–581. doi: 10.1007/BF01205220. [DOI] [PubMed] [Google Scholar]

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