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. 1980 May 1;85(2):258–272. doi: 10.1083/jcb.85.2.258

Oversized flagellar membrane protein in paralyzed mutants of Chlamydomonas reinhardrii

PMCID: PMC2110618  PMID: 7372708

Abstract

A mutant strain of Chlamydomonas reinhardtii is shown to possess an oversized flagellar membrane protein. The mutant has paralyzed flagella, is temperature sensitive for flagellar assembly, and has an abnormal axonemal protein composition. All phenotypes appear to derive from a single Mendelian mutation, and genetic analysis suggests that the mutation, which call ts222, is in the gene pfl. Because pf1 mutants are known to have radial-spoke defects (Piperno et al., 1977, Proc. Natl. Acad. Sci. U. S. A. 74:1600-1604; and Witman et al., 1978, J. Cell Biol. 76:729-797), a relation as yet undefined appears to exist between radial-spoke and flagellar membrane biogenesis.

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Selected References

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  1. Bergman K., Goodenough U. W., Goodenough D. A., Jawitz J., Martin H. Gametic differentiation in Chlamydomonas reinhardtii. II. Flagellar membranes and the agglutination reaction. J Cell Biol. 1975 Dec;67(3):606–622. doi: 10.1083/jcb.67.3.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blobel G., Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975 Dec;67(3):835–851. doi: 10.1083/jcb.67.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bloodgood R. A., Leffler E. M., Bojczuk A. T. Reversible inhibition of Chlamydomonas flagellar surface motility. J Cell Biol. 1979 Sep;82(3):664–674. doi: 10.1083/jcb.82.3.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bloodgood R. A. Motility occurring in association with the surface of the Chlamydomonas flagellum. J Cell Biol. 1977 Dec;75(3):983–989. doi: 10.1083/jcb.75.3.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bouck G. B., Rogalski A., Valaitis A. Surface organization and composition of Euglena. II. Flagellar mastigonemes. J Cell Biol. 1978 Jun;77(3):805–826. doi: 10.1083/jcb.77.3.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Castillo C. J., Hsiao C. L., Coon P., Black L. W. Identification and perperties of bacteriophage T4 capsid-formation gene products. J Mol Biol. 1977 Mar 5;110(3):585–601. doi: 10.1016/s0022-2836(77)80113-7. [DOI] [PubMed] [Google Scholar]
  7. Dawes J., Goldberg E. B. Functions of baseplate components in bacteriophage T4 infection. II. Products of genes 5, 6, 7, 8, and 10. Virology. 1973 Oct;55(2):391–396. doi: 10.1016/0042-6822(73)90179-7. [DOI] [PubMed] [Google Scholar]
  8. Dentler W. L., Rosenbaum J. L. Flagellar elongation and shortening in Chlamydomonas. III. structures attached to the tips of flagellar microtubules and their relationship to the directionality of flagellar microtubule assembly. J Cell Biol. 1977 Sep;74(3):747–759. doi: 10.1083/jcb.74.3.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ebersold W. T. Chlamydomonas reinhardi: heterozygous diploid strains. Science. 1967 Jul 28;157(3787):447–449. doi: 10.1126/science.157.3787.447. [DOI] [PubMed] [Google Scholar]
  10. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  11. Gibbons B. H., Gibbons I. R. Flagellar movement and adenosine triphosphatase activity in sea urchin sperm extracted with triton X-100. J Cell Biol. 1972 Jul;54(1):75–97. doi: 10.1083/jcb.54.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hansma H. G. The immobilization antigen of Paramecium aurelia is a single polypeptide chain. J Protozool. 1975 May;22(2):257–259. doi: 10.1111/j.1550-7408.1975.tb05861.x. [DOI] [PubMed] [Google Scholar]
  13. Hershko A., Fry M. Post-translational cleavage of polypeptide chains: role in assembly. Annu Rev Biochem. 1975;44:775–797. doi: 10.1146/annurev.bi.44.070175.004015. [DOI] [PubMed] [Google Scholar]
  14. Huang B., Rifkin M. R., Luck D. J. Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol. 1977 Jan;72(1):67–85. doi: 10.1083/jcb.72.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hunt L. A., Etchison J. R., Summers D. F. Oligosaccharide chains are trimmed during synthesis of the envelope glycoprotein of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1978 Feb;75(2):754–758. doi: 10.1073/pnas.75.2.754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jarvik J., Botstein D. A genetic method for determining the order of events in a biological pathway. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2046–2050. doi: 10.1073/pnas.70.7.2046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. King J., Lenk E. V., Botstein D. Mechanism of head assembly and DNA encapsulation in Salmonella phage P22. II. Morphogenetic pathway. J Mol Biol. 1973 Nov 15;80(4):697–731. doi: 10.1016/0022-2836(73)90205-2. [DOI] [PubMed] [Google Scholar]
  18. LEVINE R. P., EBERSOLD W. T. The genetics and cytology of Chlamydomonas. Annu Rev Microbiol. 1960;14:197–216. doi: 10.1146/annurev.mi.14.100160.001213. [DOI] [PubMed] [Google Scholar]
  19. LEWIN R. A. Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol. 1954 Dec;11(3):358–363. doi: 10.1099/00221287-11-3-358. [DOI] [PubMed] [Google Scholar]
  20. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  21. Lefebvre P. A., Nordstrom S. A., Moulder J. E., Rosenbaum J. L. Flagellar elongation and shortening in Chlamydomonas. IV. Effects of flagellar detachment, regeneration, and resorption on the induction of flagellar protein synthesis. J Cell Biol. 1978 Jul;78(1):8–27. doi: 10.1083/jcb.78.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Luck D., Piperno G., Ramanis Z., Huang B. Flagellar mutants of Chlamydomonas: studies of radial spoke-defective strains by dikaryon and revertant analysis. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3456–3460. doi: 10.1073/pnas.74.8.3456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Luftig R. B., Lundh N. P. Bacteriophage T4 head morphogenesis. Isolation, partial characterization, and fate of gene 21-defective tau-particles. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1636–1640. doi: 10.1073/pnas.70.6.1636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Piperno G., Huang B., Luck D. J. Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1600–1604. doi: 10.1073/pnas.74.4.1600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ringo D. L. Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. J Cell Biol. 1967 Jun;33(3):543–571. doi: 10.1083/jcb.33.3.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rosenbaum J. L., Moulder J. E., Ringo D. L. Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins. J Cell Biol. 1969 May;41(2):600–619. doi: 10.1083/jcb.41.2.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. SADLER J. R., NOVICK A. THE PROPERTIES OF REPRESSOR AND THE KINETICS OF ITS ACTION. J Mol Biol. 1965 Jun;12:305–327. doi: 10.1016/s0022-2836(65)80255-8. [DOI] [PubMed] [Google Scholar]
  28. SAGER R., GRANICK S. Nutritional studies with Chlamydomonas reinhardi. Ann N Y Acad Sci. 1953 Oct 14;56(5):831–838. doi: 10.1111/j.1749-6632.1953.tb30261.x. [DOI] [PubMed] [Google Scholar]
  29. Sattler C. A., Staehelin L. A. Ciliary membrane differentiations in Tetrahymena pyriformis. Tetrahymena has four types of cilia. J Cell Biol. 1974 Aug;62(2):473–490. doi: 10.1083/jcb.62.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Snell W. J. Mating in Chlamydomonas: a system for the study of specific cell adhesion. I. Ultrastructural and electrophoretic analyses of flagellar surface components involved in adhesion. J Cell Biol. 1976 Jan;68(1):48–69. doi: 10.1083/jcb.68.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stephens R. E. Major membrane protein differences in cilia and flagella: evidence for a membrane-associated tubulin. Biochemistry. 1977 May 17;16(10):2047–2058. doi: 10.1021/bi00629a001. [DOI] [PubMed] [Google Scholar]
  32. Witman G. B., Carlson K., Berliner J., Rosenbaum J. L. Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes. J Cell Biol. 1972 Sep;54(3):507–539. doi: 10.1083/jcb.54.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Witman G. B., Plummer J., Sander G. Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components. J Cell Biol. 1978 Mar;76(3):729–747. doi: 10.1083/jcb.76.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]

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