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. 1988 Apr;170(4):1978–1979. doi: 10.1128/jb.170.4.1978-1979.1988

Homocitrate cures the NifV- phenotype in Klebsiella pneumoniae.

T R Hoover 1, J Imperial 1, P W Ludden 1, V K Shah 1
PMCID: PMC211064  PMID: 3127384

Abstract

Dinitrogenase was isolated from a culture of a Klebsiella pneumoniae NifV- strain derepressed for nitrogenase in the presence of homocitrate. The enzyme isolated from this culture was identical to the wild-type dinitrogenase. These data provide in vivo evidence that the absence of homocitrate is responsible for the NifV- phenotype.

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1978

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bulen W. A., LeComte J. R. The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis. Proc Natl Acad Sci U S A. 1966 Sep;56(3):979–986. doi: 10.1073/pnas.56.3.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Filler W. A., Kemp R. M., Ng J. C., Hawkes T. R., Dixon R. A., Smith B. E. The nifH gene product is required for the synthesis or stability of the iron-molybdenum cofactor of nitrogenase from Klebsiella pneumoniae. Eur J Biochem. 1986 Oct 15;160(2):371–377. doi: 10.1111/j.1432-1033.1986.tb09981.x. [DOI] [PubMed] [Google Scholar]
  3. HOCH G. E., SCHNEIDER K. C., BURRIS R. H. Hydrogen evolution and exchange, and conversion of N2O to N2 by soybean root nodules. Biochim Biophys Acta. 1960 Jan 15;37:273–279. doi: 10.1016/0006-3002(60)90234-1. [DOI] [PubMed] [Google Scholar]
  4. Hageman R. V., Burris R. H. Nitrogenase and nitrogenase reductase associate and dissociate with each catalytic cycle. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2699–2702. doi: 10.1073/pnas.75.6.2699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hawkes T. R., McLean P. A., Smith B. E. Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor. Biochem J. 1984 Jan 1;217(1):317–321. doi: 10.1042/bj2170317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hoover T. R., Robertson A. D., Cerny R. L., Hayes R. N., Imperial J., Shah V. K., Ludden P. W. Identification of the V factor needed for synthesis of the iron-molybdenum cofactor of nitrogenase as homocitrate. 1987 Oct 29-Nov 4Nature. 329(6142):855–857. doi: 10.1038/329855a0. [DOI] [PubMed] [Google Scholar]
  7. Hoover T. R., Shah V. K., Roberts G. P., Ludden P. W. nifV-dependent, low-molecular-weight factor required for in vitro synthesis of iron-molybdenum cofactor of nitrogenase. J Bacteriol. 1986 Sep;167(3):999–1003. doi: 10.1128/jb.167.3.999-1003.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Imperial J., Ugalde R. A., Shah V. K., Brill W. J. Role of the nifQ gene product in the incorporation of molybdenum into nitrogenase in Klebsiella pneumoniae. J Bacteriol. 1984 Apr;158(1):187–194. doi: 10.1128/jb.158.1.187-194.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MacNeil T., MacNeil D., Roberts G. P., Supiano M. A., Brill W. J. Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae. J Bacteriol. 1978 Oct;136(1):253–266. doi: 10.1128/jb.136.1.253-266.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLean P. A., Dixon R. A. Requirement of nifV gene for production of wild-type nitrogenase enzyme in Klebsiella pneumoniae. Nature. 1981 Aug 13;292(5824):655–656. doi: 10.1038/292655a0. [DOI] [PubMed] [Google Scholar]
  11. Neilands J. B. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. doi: 10.1146/annurev.bi.50.070181.003435. [DOI] [PubMed] [Google Scholar]
  12. Roberts G. P., MacNeil T., MacNeil D., Brill W. J. Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae. J Bacteriol. 1978 Oct;136(1):267–279. doi: 10.1128/jb.136.1.267-279.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robinson A. C., Dean D. R., Burgess B. K. Iron-molybdenum cofactor biosynthesis in Azotobacter vinelandii requires the iron protein of nitrogenase. J Biol Chem. 1987 Oct 15;262(29):14327–14332. [PubMed] [Google Scholar]
  14. Shah V. K., Brill W. J. Isolation of a molybdenum--iron cluster from nitrogenase. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3438–3440. doi: 10.1073/pnas.78.6.3438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shah V. K., Davis L. C., Brill W. J. Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii. Biochim Biophys Acta. 1972 Feb 28;256(2):498–511. doi: 10.1016/0005-2728(72)90078-3. [DOI] [PubMed] [Google Scholar]
  16. Shah V. K., Imperial J., Ugalde R. A., Ludden P. W., Brill W. J. In vitro synthesis of the iron-molybdenum cofactor of nitrogenase. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1636–1640. doi: 10.1073/pnas.83.6.1636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shah V. K. Isolation and characterization of nitrogenase from Klebsiella pneumoniae. Methods Enzymol. 1986;118:511–519. doi: 10.1016/0076-6879(86)18097-9. [DOI] [PubMed] [Google Scholar]

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