Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Apr;170(4):1990–1993. doi: 10.1128/jb.170.4.1990-1993.1988

Hydrogen-mediated enhancement of hydrogenase expression in Azotobacter vinelandii.

J Prosser 1, L Graham 1, R J Maier 1
PMCID: PMC211068  PMID: 3280556

Abstract

Azotobacter vinelandii cultures express more H2 uptake hydrogenase activity when fixing N2 than when provided with fixed N. Hydrogen, a product of the nitrogenase reaction, is at least partly responsible for this increase. The addition of H2 to NH4+-grown wild-type cultures caused increased whole-cell H2 uptake activity, methylene blue-dependent H2 uptake activity of membranes, and accumulation of hydrogenase protein (large subunit as detected immunologically) in membranes. Both rifampin and chloramphenicol inhibited the H2-mediated enhancement of hydrogenase synthesis. Nif- A. vinelandii mutants with deletions or insertions in the nif genes responded to added H2 by increasing the amount of both whole-cell and membrane-bound hydrogenase activities. Nif- mutant strain CA11 contained fourfold more hydrogenase protein when incubated in N-free medium with H2 than when incubated in the same medium containing Ar. N2-fixing wild-type cultures that produce H2 did not increase hydrogenase protein levels in response to added H2.

Full text

PDF
1990

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arp D. J., McCollum L. C., Seefeldt L. C. Molecular and immunological comparison of membrane-bound, H2-oxidizing hydrogenases of Bradyrhizobium japonicum, Alcaligenes eutrophus, Alcaligenes latus, and Azotobacter vinelandii. J Bacteriol. 1985 Jul;163(1):15–20. doi: 10.1128/jb.163.1.15-20.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop P. E., Premakumar R., Dean D. R., Jacobson M. R., Chisnell J. R., Rizzo T. M., Kopczynski J. Nitrogen Fixation by Azotobacter vinelandii Strains Having Deletions in Structural Genes for Nitrogenase. Science. 1986 Apr 4;232(4746):92–94. doi: 10.1126/science.232.4746.92. [DOI] [PubMed] [Google Scholar]
  3. Bowien B., Schlegel H. G. Physiology and biochemistry of aerobic hydrogen-oxidizing bacteria. Annu Rev Microbiol. 1981;35:405–452. doi: 10.1146/annurev.mi.35.100181.002201. [DOI] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  5. Doyle C. M., Arp D. J. Regulation of H2 oxidation activity and hydrogenase protein levels by H2, O2, and carbon substrates in Alcaligenes latus. J Bacteriol. 1987 Oct;169(10):4463–4468. doi: 10.1128/jb.169.10.4463-4468.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedrich B., Kortlüke C., Hogrefe C., Eberz G., Silber B., Warrelmann J. Genetics of hydrogenase from aerobic lithoautotrophic bacteria. Biochimie. 1986 Jan;68(1):133–145. doi: 10.1016/s0300-9084(86)81078-1. [DOI] [PubMed] [Google Scholar]
  7. GREEN M., WILSON P. W. Hydrogenase and nitrogenase in Azotobacter. J Bacteriol. 1953 May;65(5):511–517. doi: 10.1128/jb.65.5.511-517.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Joerger R. D., Premakumar R., Bishop P. E. Tn5-induced mutants of Azotobacter vinelandii affected in nitrogen fixation under Mo-deficient and Mo-sufficient conditions. J Bacteriol. 1986 Nov;168(2):673–682. doi: 10.1128/jb.168.2.673-682.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maier R. J., Merberg D. M. Rhizobium japonicum mutants that are hypersensitive to repression of H2 uptake by oxygen. J Bacteriol. 1982 Apr;150(1):161–167. doi: 10.1128/jb.150.1.161-167.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Merberg D., O'Hara E. B., Maier R. J. Regulation of hydrogenase in Rhizobium japonicum: analysis of mutants altered in regulation by carbon substrates and oxygen. J Bacteriol. 1983 Dec;156(3):1236–1242. doi: 10.1128/jb.156.3.1236-1242.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Novak P. D., Maier R. J. Inhibition of hydrogenase synthesis by DNA gyrase inhibitors in Bradyrhizobium japonicum. J Bacteriol. 1987 Jun;169(6):2708–2712. doi: 10.1128/jb.169.6.2708-2712.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stults L. W., Moshiri F., Maier R. J. Aerobic purification of hydrogenase from Rhizobium japonicum by affinity chromatography. J Bacteriol. 1986 Jun;166(3):795–800. doi: 10.1128/jb.166.3.795-800.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wong T. Y., Maier R. J. H2-dependent mixotrophic growth of N2-fixing Azotobacter vinelandii. J Bacteriol. 1985 Aug;163(2):528–533. doi: 10.1128/jb.163.2.528-533.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wong T. Y., Maier R. J. Hydrogen-oxidizing electron transport components in nitrogen-fixing Azotobacter vinelandii. J Bacteriol. 1984 Jul;159(1):348–352. doi: 10.1128/jb.159.1.348-352.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van Berkum P. Expression of uptake hydrogenase and hydrogen oxidation during heterotrophic growth of Bradyrhizobium japonicum. J Bacteriol. 1987 Oct;169(10):4565–4569. doi: 10.1128/jb.169.10.4565-4569.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES