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. 1980 Sep 1;86(3):844–848. doi: 10.1083/jcb.86.3.844

Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis

PMCID: PMC2110690  PMID: 6157696

Abstract

A kinetic analysis of the appearance of [3H]uridine label in RNA sequences that neighbor poly(A), as well as the incorporation of [3H]adenosine label into both the RNA chain and the poly(A) of poly(A)- containing molecules, shows that poly(A) is added within a minute or so after RNA chain synthesis in Chinese hamster ovary cells and HeLa cells. Previous conclusions by several groups (5-7) that poly(A) might be added as long as 20-30 min after RNA synthesis appear to be in error, and the present conclusion seems much more in line with several different types of recent studies with specific mRNAs that suggest prompt poly(A) addition (13-16).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bastos R. N., Aviv H. Globin RNA precursor molecules: biosynthesis and process in erythroid cells. Cell. 1977 Jul;11(3):641–650. doi: 10.1016/0092-8674(77)90081-2. [DOI] [PubMed] [Google Scholar]
  2. Brawerman G. Characteristics and significance of the polyadenylate sequence in mammalian messenger RNA. Prog Nucleic Acid Res Mol Biol. 1976;17:117–148. doi: 10.1016/s0079-6603(08)60068-9. [DOI] [PubMed] [Google Scholar]
  3. Brawerman G., Diez J. Metabolism of the polyadenylate sequence of nuclear RNA and messenger RNA in mammalian cells. Cell. 1975 Jul;5(3):271–280. doi: 10.1016/0092-8674(75)90102-6. [DOI] [PubMed] [Google Scholar]
  4. Darnell J. E., Jelinek W. R., Molloy G. R. Biogenesis of mRNA: genetic regulation in mammalian cells. Science. 1973 Sep 28;181(4106):1215–1221. doi: 10.1126/science.181.4106.1215. [DOI] [PubMed] [Google Scholar]
  5. Darnell J. E., Philipson L., Wall R., Adesnik M. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971 Oct 29;174(4008):507–510. doi: 10.1126/science.174.4008.507. [DOI] [PubMed] [Google Scholar]
  6. Derman E., Darnell J. E. Relationship of chain transcription to poly(A) addition and processing of hnRNA in HeLa cells. Cell. 1974 Nov;3(3):255–264. doi: 10.1016/0092-8674(74)90140-8. [DOI] [PubMed] [Google Scholar]
  7. Derman E., Goldberg S., Darnell J. E., Jr hnRNA in HeLa cells: distribution of transcript sizes estimated from nascent molecule profile. Cell. 1976 Nov;9(3):465–472. doi: 10.1016/0092-8674(76)90091-x. [DOI] [PubMed] [Google Scholar]
  8. Goldberg S., Schwartz H., Darnell J. E., Jr Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4520–4523. doi: 10.1073/pnas.74.10.4520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harpold M. M., Evans R. M., Salditt-Georgieff M., Darnell J. E. Production of mRNA in Chinese hamster cells: relationship of the rate of synthesis to the cytoplasmic concentration of nine specific mRNA sequences. Cell. 1979 Aug;17(4):1025–1035. doi: 10.1016/0092-8674(79)90341-6. [DOI] [PubMed] [Google Scholar]
  10. Jelinek W., Adesnik M., Salditt M., Sheiness D., Wall R., Molloy G., Philipson L., Darnell J. E. Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA. J Mol Biol. 1973 Apr 15;75(3):515–532. doi: 10.1016/0022-2836(73)90458-0. [DOI] [PubMed] [Google Scholar]
  11. Jelinek W., Mollov G., Salditt M., Wall R., Sheiness D., Darnell J. E., Jr Origin of mRNA in HeLa cells and the implications for chromosome structure. Cold Spring Harb Symp Quant Biol. 1974;38:891–898. doi: 10.1101/sqb.1974.038.01.091. [DOI] [PubMed] [Google Scholar]
  12. Jelinek W., Molloy G., Fernandez-Munoz R., Salditt M., Darnell J. E. Secondary structure in heterogeneous nuclear RNA: involvement of regions from repeated DNA sites. J Mol Biol. 1974 Jan 25;82(3):361–370. doi: 10.1016/0022-2836(74)90597-x. [DOI] [PubMed] [Google Scholar]
  13. Kaufmann Y., Milcarek C., Berissi H., Penman S. HeLa cell poly(A)- mRNA codes for a subset of poly(A)+ mRNA-directed proteins with an actin as a major product. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4801–4805. doi: 10.1073/pnas.74.11.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lai C. J., Dhar R., Khoury G. Mapping the spliced and unspliced late lytic SV40 RNAs. Cell. 1978 Aug;14(4):971–982. doi: 10.1016/0092-8674(78)90351-3. [DOI] [PubMed] [Google Scholar]
  15. Mendecki J., Lee S. Y., Brawerman G. Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells. Biochemistry. 1972 Feb 29;11(5):792–798. doi: 10.1021/bi00755a018. [DOI] [PubMed] [Google Scholar]
  16. Milcarek C., Price R., Penman S. The metabolism of a poly(A) minus mRNA fraction in HeLa cells. Cell. 1974 Sep;3(1):1–10. doi: 10.1016/0092-8674(74)90030-0. [DOI] [PubMed] [Google Scholar]
  17. Nevins J. R., Darnell J. E. Groups of adenovirus type 2 mRNA's derived from a large primary transcript: probable nuclear origin and possible common 3' ends. J Virol. 1978 Mar;25(3):811–823. doi: 10.1128/jvi.25.3.811-823.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nevins J. R., Darnell J. E., Jr Steps in the processing of Ad2 mRNA: poly(A)+ nuclear sequences are conserved and poly(A) addition precedes splicing. Cell. 1978 Dec;15(4):1477–1493. doi: 10.1016/0092-8674(78)90071-5. [DOI] [PubMed] [Google Scholar]
  19. Perry R. P., Kelley D. E., LaTorre J. Synthesis and turnover of nuclear and cytoplasmic polyadenylic acid in mouse L cells. J Mol Biol. 1974 Jan 25;82(3):315–331. doi: 10.1016/0022-2836(74)90593-2. [DOI] [PubMed] [Google Scholar]
  20. Puckett L., Darnell J. E. Essential factors in the kinetic analysis of RNA synthesis in HeLa cells. J Cell Physiol. 1977 Mar;90(3):521–534. doi: 10.1002/jcp.1040900315. [DOI] [PubMed] [Google Scholar]
  21. Roop D. R., Nordstrom J. L., Tsai S. Y., Tsai M. J., O'Malley B. W. Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursors. Cell. 1978 Oct;15(2):671–685. doi: 10.1016/0092-8674(78)90035-1. [DOI] [PubMed] [Google Scholar]
  22. Ross J., Knecht D. A. Precursors of alpha and beta globin messenger RNAs. J Mol Biol. 1978 Feb 15;119(1):1–20. doi: 10.1016/0022-2836(78)90266-8. [DOI] [PubMed] [Google Scholar]
  23. Salditt-Georgieff M., Harpold M., Chen-Kiang S., Darnell J. E., Jr The addition of 5' cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are capped. Cell. 1980 Jan;19(1):69–78. doi: 10.1016/0092-8674(80)90389-x. [DOI] [PubMed] [Google Scholar]
  24. Sawicki S. G., Jelinek W., Darnell J. E. 3'-Terminal addition to HeLa cell nuclear and cytoplasmic poly (A). J Mol Biol. 1977 Jun 15;113(1):219–235. doi: 10.1016/0022-2836(77)90051-1. [DOI] [PubMed] [Google Scholar]
  25. Schibler U., Marcu K. B., Perry R. P. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells. Cell. 1978 Dec;15(4):1495–1509. doi: 10.1016/0092-8674(78)90072-7. [DOI] [PubMed] [Google Scholar]
  26. Sheiness D., Darnell J. E. Polyadenylic acid segment in mRNA becomes shorter with age. Nat New Biol. 1973 Feb 28;241(113):265–268. doi: 10.1038/newbio241265a0. [DOI] [PubMed] [Google Scholar]
  27. Sheiness D., Puckett L., Darnell J. E. Possible relationship of poly(A) shortening to mRNA turnover. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1077–1081. doi: 10.1073/pnas.72.3.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Van Ness J., Maxwell I. H., Hahn W. E. Complex population of nonpolyadenylated messenger RNA in mouse brain. Cell. 1979 Dec;18(4):1341–1349. doi: 10.1016/0092-8674(79)90244-7. [DOI] [PubMed] [Google Scholar]
  29. Weber J., Blanchard J. M., Ginsberg H., Darnell J. E., Jr Order of polyadenylic acid addition and splicing events in early adenovirus mRNA formation. J Virol. 1980 Jan;33(1):286–291. doi: 10.1128/jvi.33.1.286-291.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wilson M. C., Sawicki S. G., Salditt-Georgieff M., Darnell J. E. Adenovirus type 2 mRNA in transformed cells: map positions and difference in transport time. J Virol. 1978 Jan;25(1):97–103. doi: 10.1128/jvi.25.1.97-103.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zimmerman S. B., Sandeen D. The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Anal Biochem. 1966 Feb;14(2):269–277. doi: 10.1016/0003-2697(66)90137-0. [DOI] [PubMed] [Google Scholar]

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