Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Apr;170(4):1999–2001. doi: 10.1128/jb.170.4.1999-2001.1988

Identification of a new Bradyrhizobium japonicum gene (frxA) encoding a ferredoxinlike protein.

S Ebeling 1, J D Noti 1, H Hennecke 1
PMCID: PMC211070  PMID: 3350797

Abstract

An open reading frame of 74 codons was identified downstream of the nifB gene of Bradyrhizobium japonicum 110. The predicted amino acid sequence shared 63% similarity with the Rhodopseudomonas palustris ferredoxin I sequence. We propose to name the gene frxA. The frxA gene was found to be cotranscribed with the nifB gene. An insertion mutation within frxA hardly affected nitrogen fixation activity.

Full text

PDF
1999

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buchanan-Wollaston V., Cannon M. C., Beynon J. L., Cannon F. C. Role of the nifA gene product in the regulation of nif expression in Klebsiella pneumoniae. Nature. 1981 Dec 24;294(5843):776–778. doi: 10.1038/294776a0. [DOI] [PubMed] [Google Scholar]
  2. Buikema W. J., Klingensmith J. A., Gibbons S. L., Ausubel F. M. Conservation of structure and location of Rhizobium meliloti and Klebsiella pneumoniae nifB genes. J Bacteriol. 1987 Mar;169(3):1120–1126. doi: 10.1128/jb.169.3.1120-1126.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carter K. R., Rawlings J., Orme-Johnson W. H., Becker R. R., Evans H. J. Purification and characterization of a ferredoxin from Rhizobium japonicum bacteroids. J Biol Chem. 1980 May 10;255(9):4213–4223. [PubMed] [Google Scholar]
  4. Casadaban M. J., Martinez-Arias A., Shapira S. K., Chou J. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. Methods Enzymol. 1983;100:293–308. doi: 10.1016/0076-6879(83)00063-4. [DOI] [PubMed] [Google Scholar]
  5. Dixon R. A. The genetic complexity of nitrogen fixation. The ninth Fleming lecture. J Gen Microbiol. 1984 Nov;130(11):2745–2755. doi: 10.1099/00221287-130-11-2745. [DOI] [PubMed] [Google Scholar]
  6. Dusha I., Kovalenko S., Banfalvi Z., Kondorosi A. Rhizobium meliloti insertion element ISRm2 and its use for identification of the fixX gene. J Bacteriol. 1987 Apr;169(4):1403–1409. doi: 10.1128/jb.169.4.1403-1409.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Earl C. D., Ronson C. W., Ausubel F. M. Genetic and structural analysis of the Rhizobium meliloti fixA, fixB, fixC, and fixX genes. J Bacteriol. 1987 Mar;169(3):1127–1136. doi: 10.1128/jb.169.3.1127-1136.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. George D. G., Hunt L. T., Yeh L. S., Barker W. C. New perspectives on bacterial ferredoxin evolution. J Mol Evol. 1985;22(1):20–31. doi: 10.1007/BF02105801. [DOI] [PubMed] [Google Scholar]
  9. Hales B. J., Case E. E., Morningstar J. E., Dzeda M. F., Mauterer L. A. Isolation of a new vanadium-containing nitrogenase from Azotobacter vinelandii. Biochemistry. 1986 Nov 18;25(23):7251–7255. doi: 10.1021/bi00371a001. [DOI] [PubMed] [Google Scholar]
  10. Iismaa S. E., Watson J. M. A gene upstream of the Rhizobium trifolii nifA gene encodes a ferredoxin-like protein. Nucleic Acids Res. 1987 Apr 10;15(7):3180–3180. doi: 10.1093/nar/15.7.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Minami Y., Wakabayashi S., Yamada F., Wada K., Zumft W. G., Matsubara H. Ferredoxins from the photosynthetic purple non-sulfur bacterium Rhodopseudomonas palustris. Isolation and amino acid sequence of ferredoxin I. J Biochem. 1984 Sep;96(3):585–592. doi: 10.1093/oxfordjournals.jbchem.a134873. [DOI] [PubMed] [Google Scholar]
  12. Noti J. D., Folkerts O., Turken A. N., Szalay A. A. Organization and characterization of genes essential for symbiotic nitrogen fixation from Bradyrhizobium japonicum I110. J Bacteriol. 1986 Sep;167(3):774–783. doi: 10.1128/jb.167.3.774-783.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Prentki P., Krisch H. M. In vitro insertional mutagenesis with a selectable DNA fragment. Gene. 1984 Sep;29(3):303–313. doi: 10.1016/0378-1119(84)90059-3. [DOI] [PubMed] [Google Scholar]
  14. Regensburger B., Hennecke H. RNA polymerase from Rhizobium japonicum. Arch Microbiol. 1983 Aug;135(2):103–109. doi: 10.1007/BF00408017. [DOI] [PubMed] [Google Scholar]
  15. Roberts G. P., Brill W. J. Genetics and regulation of nitrogen fixation. Annu Rev Microbiol. 1981;35:207–235. doi: 10.1146/annurev.mi.35.100181.001231. [DOI] [PubMed] [Google Scholar]
  16. Robson R., Woodley P., Jones R. Second gene (nifH*) coding for a nitrogenase iron protein in Azotobacter chroococcum is adjacent to a gene coding for a ferredoxin-like protein. EMBO J. 1986 Jun;5(6):1159–1163. doi: 10.1002/j.1460-2075.1986.tb04341.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
  18. Tanaka M., Haniu M., Yasunobu K. T., Evans M. C., Rao K. K. Amino acid sequence of ferredoxin from a photosynthetic green bacterium, Chlorobium limicola. Biochemistry. 1974 Jul 2;13(14):2953–2959. doi: 10.1021/bi00711a026. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES