Abstract
Laser diffraction techniques coupled with simultaneous tension measurements were used to determine the length-tension relation in intact, small (0.5-mm thick, 10-mm wide, 20-25-mm long) bundles of a Limulus (horseshoe crab) striated muscle, the telson levator muscle. This muscle differs from the model vertebrate systems in that the thick filaments are not of a constant length, but shorten from 4.9 to approximately 2.0 micrometers as the sarcomeres shorten from 7 to 3 micrometers. In the Limulus muscle, the length-tension relation plateaued to an average maximum tension of 0.34 N/mm2 at a sarcomere length of 6.5 micrometers (Lo) to 8.0 micrometers. In the sarcomere length range from 3.8 to 12.5 micrometers, the muscle developed 50% or more of the maximum tension. When the sarcomere lengths are normalized (expressed as L/Lo) and the Limulus data are compared to those from frog muscle, it is apparent that Limulus muscle develops tension over a relatively greater range of sarcomere lengths.
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Selected References
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- April E. W., Brandt P. W. The myofilament lattice: studies on isolated fibers. 3. The effect of myofilament spacing upon tension. J Gen Physiol. 1973 Apr;61(4):490–508. doi: 10.1085/jgp.61.4.490. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brann L., Dewey M. M., Baldwin E. A., Brink P., Walcott B. Requirements for in vitro shortening and lengthening of isolated thick filaments of Limulus striated muscle. Nature. 1979 May 17;279(5710):256–257. doi: 10.1038/279256a0. [DOI] [PubMed] [Google Scholar]
- Cleworth D. R., Edman K. A. Changes in sarcomere length during isometric tension development in frog skeletal muscle. J Physiol. 1972 Dec;227(1):1–17. doi: 10.1113/jphysiol.1972.sp010016. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE VILLAFRANCA G. W. The A and IB and lengths in stretched or contracted horseshoe crab skeletal muscle. J Ultrastruct Res. 1961 Apr;5:109–115. doi: 10.1016/s0022-5320(61)90008-9. [DOI] [PubMed] [Google Scholar]
- Dewey M. M., Levine R. J., Colflesh D. E. Structure of Limulus striated muscle. The contractile apparatus at various sarcomere lengths. J Cell Biol. 1973 Sep;58(3):574–593. doi: 10.1083/jcb.58.3.574. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dewey M. M., Walcott B., Colflesh D. E., Terry H., Levine R. J. Changes in thick filament length in Limulus striated muscle. J Cell Biol. 1977 Nov;75(2 Pt 1):366–380. doi: 10.1083/jcb.75.2.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gordon A. M., Huxley A. F., Julian F. J. The variation in isometric tension with sarcomere length in vertebrate muscle fibres. J Physiol. 1966 May;184(1):170–192. doi: 10.1113/jphysiol.1966.sp007909. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUXLEY A. F., NIEDERGERKE R. Structural changes in muscle during contraction; interference microscopy of living muscle fibres. Nature. 1954 May 22;173(4412):971–973. doi: 10.1038/173971a0. [DOI] [PubMed] [Google Scholar]
- HUXLEY H., HANSON J. Changes in the cross-striations of muscle during contraction and stretch and their structural interpretation. Nature. 1954 May 22;173(4412):973–976. doi: 10.1038/173973a0. [DOI] [PubMed] [Google Scholar]
- Jahromi S. S., Atwood H. L. Correlation of structure, speed of contraction, and total tension in fast and slow abdominal muscle fibers of the lobster (Homarus americanus). J Exp Zool. 1969 May;171(1):25–38. doi: 10.1002/jez.1401710105. [DOI] [PubMed] [Google Scholar]
- Maruyama K., Natori R., Nonomura Y. New elastic protein from muscle. Nature. 1976 Jul 1;262(5563):58–60. doi: 10.1038/262058a0. [DOI] [PubMed] [Google Scholar]