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. 1980 Oct 1;87(1):280–284. doi: 10.1083/jcb.87.1.280

Electron microscope evidence for the presence of globular structures in different sperm chromatins

PMCID: PMC2110730  PMID: 7419596

Abstract

Dispersion of nuclear fibers of the spermatozoa of dogfish, man, and bull is made possible after treatment with a reducing and alkylating reagent coupled with an anionic detergent; the same detergent used at a low ionic strength dissociates the nuclear content of the rainbow trout sperm. Electron microscopy of such dispersed nuclear fibers has shown a beads-on-a-string configuration for these four types of sperm chromatin. These structures are morphologically similar to those described in somatic cell nuclei as nucleosomes, although in sperm chromatin the basic proteins associated with DNA were significantly different from histones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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