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. 1988 May;170(5):2027–2030. doi: 10.1128/jb.170.5.2027-2030.1988

Attachment of Pseudomonas fluorescens to glass and influence of electrolytes on bacterium-substratum separation distance.

M Fletcher 1
PMCID: PMC211081  PMID: 3129399

Abstract

The influence of Na+, Ca2+, La3+, and Fe3+ on the adhesion of Pseudomonas fluorescens H2 and H2S was investigated with interference reflection microscopy (IRM). IRM is a light microscopy technique which allows (i) visualization of the adhesive sites of living bacteria as they attach to a glass cover slip surface and (ii) evaluation of the bacterium-glass surface separation distance within a range of 0 to ca. 100 nm. The addition of each cation caused changes in IRM images consistent with a decrease in the separation distance, and minimum effective concentrations were as follows: Na+, 1 mM; Ca2+, 1 mM; La3+, 50 microM; and Fe3+, 50 microM. With strain H2, the effects of Na+, Ca2+, and La3+ were fully reversible in that the separation distance increased again when the electrolyte was replaced with distilled water. However, with strain H2S, a spontaneous mutant of H2 with increased attachment ability, only the effect of Na+ was fully reversible, and the effects of Ca2+ and La3+ were only partially reversible or irreversible. The effect of Fe3+ was irreversible with both strains, but this may be related not only to the electrolytic nature of Fe3+ but also to the decrease in solution pH to 3.5 caused by its addition. It is proposed that the electrolytes caused a decrease in separation distance by neutralizing negative charges on bacterial surface polymers and that the different effects obtained with the two strains are related to their different adhesion abilities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abercrombie M., Dunn G. A. Adhesions of fibroblasts to substratum during contact inhibition observed by interference reflection microscopy. Exp Cell Res. 1975 Apr;92(1):57–62. doi: 10.1016/0014-4827(75)90636-9. [DOI] [PubMed] [Google Scholar]
  2. CURTIS A. S. THE MECHANISM OF ADHESION OF CELLS TO GLASS. A STUDY BY INTERFERENCE REFLECTION MICROSCOPY. J Cell Biol. 1964 Feb;20:199–215. doi: 10.1083/jcb.20.2.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dexter S. C., Sullivan J. D., Williams J., Watson S. W. Influence of substrate wettability on the attachment of marine bacteria to various surfaces. Appl Microbiol. 1975 Aug;30(2):298–308. doi: 10.1128/am.30.2.298-308.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fletcher M., Marshall K. C. Bubble contact angle method for evaluating substratum interfacial characteristics and its relevance to bacterial attachment. Appl Environ Microbiol. 1982 Jul;44(1):184–192. doi: 10.1128/aem.44.1.184-192.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gingell D., Todd I. Interference reflection microscopy. A quantitative theory for image interpretation and its application to cell-substratum separation measurement. Biophys J. 1979 Jun;26(3):507–526. doi: 10.1016/S0006-3495(79)85268-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gordon A. S., Millero F. J. Electrolyte effects on attachment of an estuarine bacterium. Appl Environ Microbiol. 1984 Mar;47(3):495–499. doi: 10.1128/aem.47.3.495-499.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harber M. J., Mackenzie R., Asscher A. W. A rapid bioluminescence method for quantifying bacterial adhesion to polystyrene. J Gen Microbiol. 1983 Mar;129(3):621–632. doi: 10.1099/00221287-129-3-621. [DOI] [PubMed] [Google Scholar]
  8. Izzard C. S., Lochner L. R. Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique. J Cell Sci. 1976 Jun;21(1):129–159. doi: 10.1242/jcs.21.1.129. [DOI] [PubMed] [Google Scholar]
  9. King C. A., Heaysman J. E., Preston T. M. Experimental evidence for the role of long range forces in fibroblast-substrate interaction. Exp Cell Res. 1979 Mar 15;119(2):406–410. doi: 10.1016/0014-4827(79)90373-2. [DOI] [PubMed] [Google Scholar]
  10. McEldowney S., Fletcher M. Variability of the influence of physicochemical factors affecting bacterial adhesion to polystyrene substrata. Appl Environ Microbiol. 1986 Sep;52(3):460–465. doi: 10.1128/aem.52.3.460-465.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Paul J. H. Effects of antimetabolites on the adhesion of an estuarine Vibrio sp. to polystyrene. Appl Environ Microbiol. 1984 Nov;48(5):924–929. doi: 10.1128/aem.48.5.924-929.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Preston T. M., King C. A. An experimental study of the interaction between the soil amoeba Naegleria gruberi and a glass substrate during amoeboid locomotion. J Cell Sci. 1978 Dec;34:145–158. doi: 10.1242/jcs.34.1.145. [DOI] [PubMed] [Google Scholar]
  13. Pringle J. H., Fletcher M. Influence of substratum wettability on attachment of freshwater bacteria to solid surfaces. Appl Environ Microbiol. 1983 Mar;45(3):811–817. doi: 10.1128/aem.45.3.811-817.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stanley P. M. Factors affecting the irreversible attachment of Pseudomonas aeruginosa to stainless steel. Can J Microbiol. 1983 Nov;29(11):1493–1499. doi: 10.1139/m83-230. [DOI] [PubMed] [Google Scholar]
  15. Sutherland I. W. Biosynthesis of microbial exopolysaccharides. Adv Microb Physiol. 1982;23:79–150. doi: 10.1016/s0065-2911(08)60336-7. [DOI] [PubMed] [Google Scholar]

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