Skip to main content
PMC home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1976 Apr 1;69(1):205–210. doi: 10.1083/jcb.69.1.205

Carbamycholine prevents giant granule-formation in cultured fibroblasts from beige (Chediak-Higashi) mice

PMCID: PMC2110978  PMID: 1254644

Abstract

Primary embryonic fibroblasts isolated from beige (Chediak-Higashi) mice develop pathognomonic giant granules in vitro. Inclusion in the culture medium of carbamylcholine (carbachol) or phorbol myristate acetate (PMA) results in the generation of morphologically normally granules. Chediak-Higashi fibroblasts are highly susceptible to shape changes induced by colchicine. This abnormal property is also corrected by carbachol and PMA.

Full Text

The Full Text of this article is available as a PDF (1.9 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J. M., Blume R. S., Wolff S. M. Characterization and significance of abnormal leukocyte granules in the beige mouse: a possible homologue for Chediak-Higashi Aleutian trait. J Lab Clin Med. 1969 Feb;73(2):235–243. [PubMed] [Google Scholar]
  2. COHN Z. A., BENSON B. THE IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. II. THE INFLUENCE OF SERUM ON GRANULE FORMATION, HYDROLASE PRODUCTION, AND PINOCYTOSIS. J Exp Med. 1965 May 1;121:835–848. doi: 10.1084/jem.121.5.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davis W. C., Spicer S. S., Greene W. B., Padgett G. A. Ultrastructure of bone marrow granulocytes in normal mink and mink with the homolog of the Chediak-Higashi trait of humans. I. Origin of the abnormal granules present in the neutrophils of mink with the C-HS trait. Lab Invest. 1971 Apr;24(4):303–317. [PubMed] [Google Scholar]
  4. Estensen R. D., Hill H. R., Quie P. G., Gogan N., Goldberg N. D. Cyclic GMP and cell movement. Nature. 1973 Oct 26;245(5426):458–460. doi: 10.1038/245458a0. [DOI] [PubMed] [Google Scholar]
  5. Gallin J. I., Bujak J. S., Patten E., Wolff S. M. Granulocyte function in the Chediak-Higashi syndrome of mice. Blood. 1974 Feb;43(2):201–206. [PubMed] [Google Scholar]
  6. Goldstein I., Hoffstein S., Gallin J., Weissmann G. Mechanisms of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2916–2920. doi: 10.1073/pnas.70.10.2916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hecker E. Cocarcinogenic principles from the seed oil of Croton tiglium and from other Euphorbiaceae. Cancer Res. 1968 Nov;28(11):2338–2349. [PubMed] [Google Scholar]
  8. Hickman S., Neufeld E. F. A hypothesis for I-cell disease: defective hydrolases that do not enter lysosomes. Biochem Biophys Res Commun. 1972 Nov 15;49(4):992–999. doi: 10.1016/0006-291x(72)90310-5. [DOI] [PubMed] [Google Scholar]
  9. Lutzner M. A., Tierney J. H., Benditt E. P. Giant granules and widespread cytoplasmic inclusions in a genetic syndrome of Aleutian mink. An electron microscopic study. Lab Invest. 1965 Dec;14(12):2063–2079. [PubMed] [Google Scholar]
  10. Oliver J. M., Ukena T. E., Berlin R. D. Effects of phagocytosis and colchicine on the distribution of lectin-binding sites on cell surfaces. Proc Natl Acad Sci U S A. 1974 Feb;71(2):394–398. doi: 10.1073/pnas.71.2.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oliver J. M., Zurier R. B., Berlin R. D. Concanavalin a cap formation on polymorphonuclear leukocytes of normal and beige (chediak-higashi) mice. Nature. 1975 Feb 6;253(5491):471–473. doi: 10.1038/253471a0. [DOI] [PubMed] [Google Scholar]
  12. PADGETT G. A., LEADER R. W., GORHAM J. R., O'MARY C. C. THE FAMILIAL OCCURRENCE OF THE CHEDIAK-HIGASHI SYNDROME IN MINK AND CATTLE. Genetics. 1964 Mar;49:505–512. doi: 10.1093/genetics/49.3.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rozenszajn L. A., Radnay J. The lysosomal nature of the anomalous granules and chromosome aberrations in cultures of peripheral blood in Chediak-Higashi syndrome. Br J Haematol. 1970 Jun;18(6):683–689. doi: 10.1111/j.1365-2141.1970.tb01593.x. [DOI] [PubMed] [Google Scholar]
  14. Rudland P. S., Seeley M., Seifert W. Cyclic GMP and cyclic AMP levels in normal and transformed fibroblasts. Nature. 1974 Oct 4;251(5474):417–419. doi: 10.1038/251417a0. [DOI] [PubMed] [Google Scholar]
  15. Ukena T. E., Berlin R. D. Effect of colchicine and vinblastine on the topographical separation of membrane functions. J Exp Med. 1972 Jul 1;136(1):1–7. doi: 10.1084/jem.136.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weissmann G., Goldstein I., Hoffstein S., Tsung P. K. Reciprocal effects of cAMP and cGMP on microtubule-dependent release of lysosomal enzymes. Ann N Y Acad Sci. 1975 Jun 30;253:750–762. doi: 10.1111/j.1749-6632.1975.tb19243.x. [DOI] [PubMed] [Google Scholar]
  17. Wolff S. M. The Chediak-Higashi syndrome: studies of host defenses. Ann Intern Med. 1972 Feb;76(2):293–306. doi: 10.7326/0003-4819-76-2-293. [DOI] [PubMed] [Google Scholar]
  18. Zelenin A. V. Fluorescence microscopy of lysosomes and related structures in living cells. Nature. 1966 Oct 22;212(5060):425–426. doi: 10.1038/212425a0. [DOI] [PubMed] [Google Scholar]
  19. Zucker M. B., Troll W., Belman S. The tumor-promoter phorbol ester (12-O-tetradecanoyl-phorbol-13-acetate), a potent aggregating agent for blood platelets. J Cell Biol. 1974 Feb;60(2):325–336. doi: 10.1083/jcb.60.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES