Abstract
Nuclei prepared from MOPC-21 cells were treated with the nonionic detergents Triton X-100 or Nonidet P-40. Chemical analysis revealed that nearly 90% of the nuclear phospholipid was removed by detergent treatment. The membrane-denuded nuclei remained intact with preservation of nuclear pore complexes as demonstrated by electron microscopy. Ribonucleic acid transport from detergent-treated nuclei proceeded at the same rate and to the same extent as in control nuclei. Normal nuclear restriction of nucleic acids was unaltered by removal of the nuclear membranes. The effect of temperature on transport of RNA from freshly isolated myeloma nuclei with intact nuclear envelopes was studied. No temperature transition was associated with the transport process. These data indicate that the transport of macromolecules from isolated myeloma nuclei is independent of the nuclear membrane.
Full Text
The Full Text of this article is available as a PDF (1.4 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aaronson R. P., Blobel G. Isolation of nuclear pore complexes in association with a lamina. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1007–1011. doi: 10.1073/pnas.72.3.1007. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aaronson R. P., Blobel G. On the attachment of the nuclear pore complex. J Cell Biol. 1974 Sep;62(3):746–754. doi: 10.1083/jcb.62.3.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVISON P. F., MERCER E. H. Electron microscopy of cell nuclei isolated in aqueous media. Exp Cell Res. 1956 Aug;11(1):237–239. doi: 10.1016/0014-4827(56)90215-4. [DOI] [PubMed] [Google Scholar]
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- Franke W. W. On the universality of nuclear pore complex structure. Z Zellforsch Mikrosk Anat. 1970;105(3):405–429. doi: 10.1007/BF00335464. [DOI] [PubMed] [Google Scholar]
- Jacob J., Danieli G. A. Electron microscope observations on nuclear pore-polysome association. Cell Differ. 1972 Jun;1(2):119–125. doi: 10.1016/0045-6039(72)90034-6. [DOI] [PubMed] [Google Scholar]
- Keller J. M., Riley D. E. Nuclear ghosts: a nonmembranous structural component of mammalian cell nuclei. Science. 1976 Jul 30;193(4251):399–401. doi: 10.1126/science.935874. [DOI] [PubMed] [Google Scholar]
- Linden C. D., Wright K. L., McConnell H. M., Fox C. F. Lateral phase separations in membrane lipids and the mechanism of sugar transport in Escherichia coli. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2271–2275. doi: 10.1073/pnas.70.8.2271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paine P. L., Moore L. C., Horowitz S. B. Nuclear envelope permeability. Nature. 1975 Mar 13;254(5496):109–114. doi: 10.1038/254109a0. [DOI] [PubMed] [Google Scholar]
- Raison J. K., Lyons J. M., Mehlhorn R. J., Keith A. D. Temperature-induced phase changes in mitochondrial membranes detected by spin labeling. J Biol Chem. 1971 Jun 25;246(12):4036–4040. [PubMed] [Google Scholar]
- Raison J. K., Lyons J. M., Thomson W. W. The influence of membranes on the temperature-induced changes in the kinetics of some respiratory enzymes of mitochondria. Arch Biochem Biophys. 1971 Jan;142(1):83–90. doi: 10.1016/0003-9861(71)90261-x. [DOI] [PubMed] [Google Scholar]
- Riley D. E., Keller J. M., Byers B. The isolation and characterization of nuclear ghosts from cultured HeLa cells. Biochemistry. 1975 Jul;14(13):3005–3013. doi: 10.1021/bi00684a033. [DOI] [PubMed] [Google Scholar]
- Singer R. H., Penman S. Messenger RNA in HeLa cells: kinetics of formation and decay. J Mol Biol. 1973 Aug 5;78(2):321–334. doi: 10.1016/0022-2836(73)90119-8. [DOI] [PubMed] [Google Scholar]
- Stuart S. E., Rottman F. M., Patterson R. J. Nuclear restriction of nucleic acids in the presence of ATP. Biochem Biophys Res Commun. 1975 Jan 20;62(2):439–447. doi: 10.1016/s0006-291x(75)80158-6. [DOI] [PubMed] [Google Scholar]