Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 May;170(5):2159–2162. doi: 10.1128/jb.170.5.2159-2162.1988

Anaerobic metabolism of the L-rhamnose fermentation product 1,2-propanediol in Salmonella typhimurium.

N Obradors 1, J Badía 1, L Baldomà 1, J Aguilar 1
PMCID: PMC211101  PMID: 3283105

Abstract

When grown anaerobically on L-rhamnose, Salmonella typhimurium excreted 1,2-propanediol as a fermentation product. Upon exhaustion of the methyl pentose, 1,2-propanediol was recaptured and further metabolized, provided the culture was kept under anaerobic conditions. n-Propanol and propionate were found in the medium as end products of this process at concentrations one-half that of 1,2-propanediol. As in Klebsiella pneumoniae (T. Toraya, S. Honda, and S. Fukui, J. Bacteriol. 139:39-47, 1979), a diol dehydratase which transforms 1,2-propanediol to propionaldehyde and the enzymes involved in a dismutation that converts propionaldehyde to n-propanol and propionate were induced in S. typhimurium cultures able to transform 1,2-propanediol anaerobically.

Full text

PDF
2159

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Zarban S., Heffernan L., Nishitani J., Ransone L., Wilcox G. Positive control of the L-rhamnose genetic system in Salmonella typhimurium LT2. J Bacteriol. 1984 May;158(2):603–608. doi: 10.1128/jb.158.2.603-608.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Badía J., Ros J., Aguilar J. Fermentation mechanism of fucose and rhamnose in Salmonella typhimurium and Klebsiella pneumoniae. J Bacteriol. 1985 Jan;161(1):435–437. doi: 10.1128/jb.161.1.435-437.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boronat A., Aguilar J. Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme. J Bacteriol. 1979 Nov;140(2):320–326. doi: 10.1128/jb.140.2.320-326.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chiu T. H., Feingold D. S. L-rhamnulose 1-phosphate aldolase from Escherichia coli. Crystallization and properties. Biochemistry. 1969 Jan;8(1):98–108. doi: 10.1021/bi00829a015. [DOI] [PubMed] [Google Scholar]
  6. Cocks G. T., Aguilar T., Lin E. C. Evolution of L-1, 2-propanediol catabolism in Escherichia coli by recruitment of enzymes for L-fucose and L-lactate metabolism. J Bacteriol. 1974 Apr;118(1):83–88. doi: 10.1128/jb.118.1.83-88.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ENGLESBERG E., BARON L. S. Mutation to L-rhamnose resistance and transduction to L-rhamnose utilization in Salmonella typhosa. J Bacteriol. 1959 Nov;78:675–686. doi: 10.1128/jb.78.5.675-686.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GASTON L. W., STADTMAN E. R. Fermentation of ethylene glycol by Clostridium glycolicum, sp. n. J Bacteriol. 1963 Feb;85:356–362. doi: 10.1128/jb.85.2.356-362.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hou C. T., Patel R. N., Laskin A. I., Barnabe N., Barist I. Purification and properties of a NAD-linked 1,2-propanediol dehydrogenase from propane-grown Pseudomonas fluorescens NRRL B-1244. Arch Biochem Biophys. 1983 May;223(1):297–308. doi: 10.1016/0003-9861(83)90595-7. [DOI] [PubMed] [Google Scholar]
  10. Jones D. T., Woods D. R. Acetone-butanol fermentation revisited. Microbiol Rev. 1986 Dec;50(4):484–524. doi: 10.1128/mr.50.4.484-524.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. McDonald H. C., Takeguchi M. M., Detar C. C., Simon P. A., Livsey K. A., Odstrchel G., Kaplan N. O., Weetall H. H. The isolation and characterization of a 1,2-propanediol oxidoreductase from Neisseria gonorrhoeae. J Gen Microbiol. 1980 Aug;119(2):451–458. doi: 10.1099/00221287-119-2-451. [DOI] [PubMed] [Google Scholar]
  13. OLSON J. A. The purification and properties of yeast isocitric lyase. J Biol Chem. 1959 Jan;234(1):5–10. [PubMed] [Google Scholar]
  14. Rudolph F. B., Purich D. L., Fromm H. J. Coenzyme A-linked aldehyde dehydrogenase from Escherichia coli. I. Partial purification, properties, and kinetic studies of the enzyme. J Biol Chem. 1968 Nov 10;243(21):5539–5545. [PubMed] [Google Scholar]
  15. Sridhara S., Wu T. T., Chused T. M., Lin E. C. Ferrous-activated nicotinamide adenine dinucleotide-linked dehydrogenase from a mutant of Escherichia coli capable of growth on 1, 2-propanediol. J Bacteriol. 1969 Apr;98(1):87–95. doi: 10.1128/jb.98.1.87-95.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sridhara S., Wu T. T. Purification and properties of lactaldehyde dehydrogenase from Escherichia coli. J Biol Chem. 1969 Oct 10;244(19):5233–5238. [PubMed] [Google Scholar]
  17. TAKAGI Y., SAWADA H. THE METABOLISM OF L-RHAMNOSE IN ESCHERICHIA COLI. I. L-RHAMNOSE ISOMERASE. Biochim Biophys Acta. 1964 Oct 23;92:10–17. doi: 10.1016/0926-6569(64)90263-9. [DOI] [PubMed] [Google Scholar]
  18. TAKAGI Y., SAWADA H. THE METABOLISM OF L-RHAMNOSE IN ESCHERICHIA COLI. II. L-RHAMNULOSE KINASE. Biochim Biophys Acta. 1964 Oct 23;92:18–25. doi: 10.1016/0926-6569(64)90264-0. [DOI] [PubMed] [Google Scholar]
  19. Toraya T., Honda S., Fukui S. Fermentation of 1,2-propanediol with 1,2-ethanediol by some genera of Enterobacteriaceae, involving coenzyme B12-dependent diol dehydratase. J Bacteriol. 1979 Jul;139(1):39–47. doi: 10.1128/jb.139.1.39-47.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Toraya T., Ushio K., Fukui S., Hogenkamp P. C. Studies on the mechanism of the adenosylcobalamin-dependent diol dehydrase reaction by the use of analogs of the coenzyme. J Biol Chem. 1977 Feb 10;252(3):963–970. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES